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The impact of team autonomy, reflexivity and participation on employees' mental health
Mandousová, Michaela ; Šípová, Ivana (advisor) ; Mudrák, Jiří (referee)
Mental health is an important topic in the context of the work environment. Therefore, the thesis examines the impact of team autonomy, reflexivity, and participation on employees' mental health. The theoretical part focuses on the topic of mental health, the JD-R model, burnout syndrome, engagement, and team factors with a focus on autonomy, reflexivity, and participation. The empirical part aims to test whether team autonomy, reflexivity, and participation together with burnout syndrome score or work engagement are significant predictors of burnout syndrome and engagement measured one year apart. The data used to test the stated hypotheses were obtained within the framework of the international H-WORK project, which conducted a field-based longitudinal study. The project was initiated in response to growing mental health problems in the working population in Europe. This paper uses data from two companies that underwent restructuring during the project. Because of this, there was a large drop out. The data in the research part, which was subjected to linear regression analysis, was collected from 59 respondents. The results did not support the stated hypotheses. This result may be influenced by several factors, such as the high experimental mortality already mentioned. Key words: mental health;...
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A high-temperature LC-MS method for bottom-up proteomic analyses with reduced artifacts
Starovoit, Mykyta ; Lenčo, Juraj (advisor) ; Sklenářová, Hana (referee)
Charles University, Faculty of Pharmacy Department of Analytical Chemistry Candidate: Mykyta Starovoit Supervisor: PharmDr. Juraj Lenčo, Ph.D. Title of diploma thesis: A high-temperature LC-MS method for bottom-up proteomic analyses with reduced artifacts Proteomic bottom-up LC-MS analyses need more efficient chromatographic separation to keep up with the advances in mass spectrometry and fully exploit the potential of state-of- the-art MS instruments. Elevation of column temperature represents one of the most powerful and cost-effective means for improvement of separation performance. However, high temperature also promotes in-column modification of peptides, putting a spoke in the wheel of sophisticated proteomic analyses. The current method aims to minimize the formation of temperature-related artifacts via a novel high-flow trap-elute setup with differential column heating. The trap-elute setup reduces the time peptides spend in the heated separation column, resulting in fewer generated artifacts. This mitigates an important drawback of the high column temperature. At the same time, it does not diminish its positive effect on the separation performance. Consequently, the utilization of the elevated column temperature becomes more profitable. The proposed method reduced the artifact abundance...
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Nonindigenous amphibian and reptile species of the Near and Middle East
Pazderková, Marie ; Šmíd, Jiří (advisor) ; Brejcha, Jindřich (referee)
This literary review summarizes information about twelve reptile and amphibian species, which are nonindigenous for the Near and Middle East. The studied area comprises the following states: Bahrain, Egypt, Iraq, Iran, Israel, Jordan, Qatar, Kuwait, Lebanon, Oman, Yemen, Saudi Arabia, Syria, Turkey, United Arab Emirates, Palestine. Nonindigenous species are introduced to new localities mainly via human causation. It can be either unintentional, such as transportation of the animal along with agricultural crops or exotic plants, or intentional, such as releasing the animal into the wild by breeder. Introduction of nonindigenous species can have unfortunate consequences for the stability of the indigenous ecosystem. From a human point of view, nonindigenous species can negatively affect local agriculture. Key words: Nonindigenous species, invasive species, Near East, Middle East, ecosystem, introduction, reptile, amphibian
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Impact of selected probiotic bacteria on juvenile host growth in gnotobiotic mouse model
Drgoňová, Veronika ; Janďourek, Ondřej (advisor) ; Konečná, Klára (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Pharmacy Author: Veronika Drgoňová Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Consultant: Mgr. Martin Schwarzer, Ph.D. Title of diploma: Impact of selected probiotic bacteria on juvenile host growth in gnotobiotic mouse model This thesis expands understanding of the ability of Lactiplantibacillus plantarum WJL bacteria to support the growth of gnotobiotic hosts in models of chronic malnutrition. It was investigated whether other bacteria also have similar capabilities and whether these traits are species- and strain-specific. Strains of Bifidobacterium longum ssp. longum and Bifidobacterium adolescentis were tested in a gnotobiotic juvenile mouse model of chronic malnutrition and their impact on the host's somatotropic axis was monitored. Concurrently, it was analysed how malnutrition affects the bacteria in the host's gut and the level of its colonization. Germ-free C57BL/6J mice were mono-colonized via intragastric gavage with the aforementioned bacterial strains. The mice were mated after confirming stable colonization and F1 male offspring were weaned on the 21st day after birth onto an experimental diet with low protein and fat content. Their growth was monitored weekly by...
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Neurological effects of endocrine disruptors
Ondráčková, Simona ; Brožka, Hana (advisor) ; Vítků, Jana (referee)
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The effect of menstrual cycle and cortisol and testosteron levels on strenght performance in female athletes
Novotná, Kateřina ; Heller, Jan (advisor) ; Pěkný, Martin (referee)
This study provides a comprehensive insight into the physiological and hormonal changes in female athletes during the menstrual cycle and their impact on performance training and recovery. It covers an overview of menstrual cycle physiology, the effects of steroid hormones on the female reproductive system, cortisol secretion examination, and the role of testosterone in female physiology. Furthermore, it analyzes the influence of individual menstrual cycle phases on the strength capabilities of female athletes and studies the relationship between cortisol and testosterone secretion and these abilities. It discusses the influence of the follicular phase on performance in the Wingate test, negative correlation between cortisol concentration and female athletes' strength performance, and differences in maximum performance between athletes with regular and irregular cycles. It highlights general limitations of research on women that need to be considered. Overall, this work contributes to the discussion on the relationship between hormonal changes and strength performance in female athletes, with potential future applications in training and sports performance. KEY WORDS menstrual cycle, hormones, strength capabilities, cortisol, testosterone, physical exertion
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STAT3 signalling pathway modulation and its impacts on cell phenotype, senescence and secretome.
Novotný, Ondřej ; Reiniš, Milan (advisor) ; Šmahel, Michal (referee)
Cellular senescence represents an effective barrier to tumour growth on the one hand, on the other hand, senescent cells secrete substances that create a protumourigenic environment. Understanding and influencing these processes will bring new approaches in the fight against tumours and other serious diseases. The IL-6/JAK2/STAT3 signalling pathway is crucial both in oncogenesis and in the induction of cellular senescence and it also has a major influence on the composition of the secretome of senescent cells. Targeted blockade of this pathway could therefore change the tumour microenvironment, either by directly affecting the induction of senescence itself, or by adjusting secretion and thereby by attenuation of the immunosuppressive environment induced by produced cytokines. The aim of this thesis is to find out whether and how inhibitors of the STAT3 signalling pathway can change the secretome of senescent and proliferating tumour cells. Based on the comparison of the toxicity and effectiveness of the STAT3 signalling pathway inhibition by Stattic analogues on proliferating TC-1 tumour cells, I selected two suitable inhibitor molecules for further experiments. I found out that these selected inhibitors significantly reduce the secretion of important pro-tumour signalling molecules by TC-1 tumour...
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The impact of bleeding disorders on patients' daily activities
Vacková, Eliška ; Nikodemová, Hana (advisor) ; Neugebauer, Jan (referee)
This bachelor thesis deals with the impact of bleeding disorders on patinets daily activities. Bleeding disorders represent a wide spectrum of disorders, and each disorder may differ in its etiology, clinical manifestations or subsequent therapy. For this reason, this bachelor thesis focuse primarily on haemophilia. The aim of this thesis was to investigate in which areas and to what extent the bleeding disorder affects the daily life of patients. The thesis id divide into a theoretical and an empirical part. In the theoretical part, the different bleeding conditions are characterized and von Willebrand's disease and haemophilia are described in more detail. At the end of the theoretical part, the activities of daily life and the needs of person are described. The empirical part contains the results of the own investigation, statistical analysis and comparsion of the rsults of the own work with the results of previously conducted studies. Method: to obtain the results, a questionnaire of our own design was developer, which was subsequently distributed in the Institute of hematology and blood transfusion to a selected group of respondets attending outpatient check-ups at the Centre for thrombosis and haemostasis. Results: 42 respondents took part in the questionnaire survey and it was found that...
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Effect of M1043 on the expression of inflammatory markers in a mouse model of NASH - immunohistochemical analysis
Hušková, Eliška ; Němečková, Ivana (advisor) ; Kovařík, Miroslav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Biological and Medical Sciences Study program: Laboratory diagnostics in healthcare Author: Eliska Huskova Supervisor: RNDr. Ivana Nemeckova, Ph.D. Title of thesis: The impact of M1043 on the expression of inflammatory markers in a mouse model of NASH - immunohistochemical analysis Introduction and aim of the study: The liver is an organ with many important functions in the body, such as detoxification and regulation of lipid metabolism. Impairment of these processes can lead to NASH, a disease associated with obesity and metabolic syndrome, characterized by inflammation and accumulation of fat droplets in the liver. Studies focused on NASH are important for understanding the pathophysiology and developing new therapeutic strategies. The use of mouse models allows the investigation of pathogenesis and testing of different therapeutic approaches. This study examines the effect of the anti-endoglin antibody M1043 on the expression of inflammatory markers in a mouse model of NASH. Methodology: Mice were fed a diet inducing NASH and divided into two groups, one receiving rat IgG antibody and the other one, receiving the anti-endoglin antibody M1043. The livers were collected and processed using immunohistochemical...
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Ketogenic diet as a treatment for inherited disorders of ATP synthase
Kudrnovská, Barbora ; Mráček, Tomáš (advisor) ; Tesařová, Markéta (referee)
The Tmem70 protein plays a significant role in the biogenesis of ATP synthase, mutations in the Tmem70 gene are a common cause of mitochondrial disease and have been associated with neonatal encephalocardiomyopathy. Similar to other mitochondrial disorders, there is currently no available treatment for Tmem70 defects. However, the ketogenic diet (KD) appears to be a potential therapeutic intervention for various mitochondrial disorders, and our preliminary results demonstrate its positive impact in a mouse model of Tmem70 dysfunction. Therefore, using this Tmem70 model, we decided to test a modified KD, which could be a better alternative for a potential application in human patients. The effect of the modified KD was examined in a mouse model of tamoxifen-induced whole-body knockout of Tmem70 (Tmem70 KO). The modified KD led to improved survival, slowed weight loss, and normalization of plasma indicators of liver damage. We detected a positive effect of the modified KD on the assembly of ATP synthase in liver tissue, however, skeletal muscle showed only a minimal response. Based on the results of this study, we infer that although the absence of functional Tmem70 affects both liver and muscle tissues, the greater impact on the phenotypic manifestation in the Tmem70 KO mouse model is due to liver...
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