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Růst Mycobacterium smegmatis na agarovém médiu a agarovém médiu pokrytém celofánovou folií - morfologická a proteomová studie
Ramaniuk, Volha ; Weiser, Jaroslav (advisor) ; Beranová, Jana (referee)
Biofilm formation is one of the most common bacterial survival strategies. Majority of bacterial species are able to form these three-dimensional structures, including pathogens like Mycobacterium tuberculosis. Representatives of Mycobacterium genus widely occur in the nature, although they can cause serious problems when they appear in medical equipment and artificial replacements of the human body. Non-pathogenic Mycobacterium smegmatis mc2 155 was used as a model organism in our experiments. We investigated morphology of the three- and six-day-old colonies (in fact biofilms) on agar and agar covered with cellophane using Stereo microscope and Scanning Electron Microscope. We found that a type of surface as well as a carbon source has a great influence on the morphology of the M. smegmatis colonies. We isolated proteomes from the agar and cellophane cultures and from planktonic culture. Two-dimensional electrophoresis was used as the main proteomic method. Proteomic data were analyzed using PDQuest software. Then the sets of proteins detected by qualitative and quantitative analyses were compared using Venn diagrams. As a result, we recognized 7 unique proteins that might be specific for recognition and adhesion of bacteria to the cellophane, no unique protein in agar proteome and 46 unique...
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Implementation of philosophy of CRM in selected company in airline industry
Pavelka, Jakub ; Koliš, Karel (advisor) ; Konvalinka, Michal (referee)
The aim of this Bachelors Thesis is to define the philosophy, individual phases and the most important principles in building the customer relationship management and their theoretical concepts applied in selected company in airline industry. Based on the identification of places and processes that have the most significant impact on interaction between customers and company will be formulated fundamental measures for the implementation of the philosophy.
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The role of nurse in the care of child patient with hemangiom.
ŠESTÁKOVÁ, Růžena
One of the most important areas of society-wide tasks influencing the health of the whole population is child care. It includes care of children with a hemangioma. Hemangiomas are the most common benign (not malignant) vascular tumours of a child age. This benign tumour may be present as early as at birth or appear in the first weeks of life. It is usually located on or under the skin surface but may also affect internal organs. Although a hemangioma may affect any body part, it is mostly located on the head and neck. A hemangioma is diagnosed in a tenth of new-born children. Hemangiomas typically appear at neonatal age or infancy. The objectives of the diploma thesis "Nurse's Roles in Care of a Child Patient with a Hemangioma" are to examine the roles of nurses in care of a child patient with a hemangioma, as seen by parents (mothers) of the children, and to identify which of the roles is the most important for the parents (mothers). It has also been ascertained whether nurses deal with any problems while providing this care and whether occurrence of a hemangioma in a child has any influence on the child's family. It has also been ascertained whether any complications are encountered in the treatment of children with a hemangioma. A qualitative research was used for data processing. Several methods of data collection were used. The researcher used a case history according to the model of Virginia Henderson, semi-structured interview with mothers of patients, data content analysis and secret participant observation during outpatient checks. The last method used was a survey aimed at nurses who took care of children with a hemangioma, asking them whether they dealt with any problems and complications in such children. The first research set consisted of ten child patients who were selected on purpose on the basis of pre-set criteria. The first criterion was presence of a hemangioma; five patients were hospitalised with a hemangioma and underwent a treatment with propranolol, while the other five patients with a hemangioma underwent only a conservative treatment. Last but the most important criterion was that mothers of patients with a hemangioma granted their consent to the research. These mothers formed the second research set and underwent a semi-structured interview focused on their personal experience with a hemangioma and on the work of nurses as seen by mothers. The last research set consisted of ten nurses who were also selected on purpose on the basis of a very important criterion, which was care of child patients with a hemangioma. This thesis may be of a benefit not only for professionals who are in contact with children with a hemangioma but may also serve to deepen general knowledge of other healthcare professionals who may encounter this disease either in their professional or private life. Based on the information acquired for the diploma thesis, was prepared for nurses and will be presented at professional workshops.
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World Trade in Crude Oil
Benešová, Nela ; Čajka, Radek (advisor) ; Bolotov, Ilya (referee)
This master´s thesis deals with trade with crude oil and its impact on the world economy. Everyone, who wants to start to trade with crude oil, should find out basic terminology and information about crude oil that is characterized in the first chapter. The hunt for oil started many years ago, therefore the history is also mentioned in this master´s thesis. The second chapter focuses on the world trade with crude oil. Territorial structure and trade flows are described in this part of the master´s thesis. Nevertheless, the crude oil also has an impact on the world economy and economies of single states. The third chapter analyses states, where the inhabitants live beyond means of people from other countries but these states are so depended on crude oil that if this trade stopped, these countries would go bankrupt. However, there are also exceptions. Even though, there are states that have huge sources of crude oil, they still have to deal with financial problems. The forth chapter characterizes the trade with crude oil of the Czech Republic because it is a net importer of crude oil and the last chapter focuses on new trends in crude oil such as shale oil and trading at a crude oil market.
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Analysis of Reporting in Business and its Technological Coverage by Microsoft BI
Lučan, Martin ; Pour, Jan (advisor) ; Pavlíčková, Jarmila (referee)
This master thesis deals with an analysis of reporting in business and its technological coverage by Microsoft Business Intelligence portfolio. The main objective of this work is to analyze the individual organization's requirements on reporting and coverage options. This work can serve as a tool for implementation of reporting in a company or for increasing the efficiency of reporting. The first part is theoretical. It deals with Business Intelligence as an environment for reporting. It also defines the basic concepts. The next part covers the area of reporting. This section provides an insight into the history, definition of outcomes and detailed classification of reporting from different perspectives. Furthermore, the definition of users reporting and the reporting standards in companies is also covered. At the end of this chapter, there is the definition of the benefits of reporting for companies. The main part of this master thesis is an analysis of the requirements for reporting in companies. The chapter defines five key perspectives which are described in a greater detail. The methods how companies should methodically approach these requirements are also discussed. The chapter further looks at the requirements for a concrete report. An output of this chapter is an effective template for gathering requirements for a specific report. The last chapter focuses on reporting portfolio analysis of Microsoft and defines the concept of Microsoft. It provides detailed information about reporting products that Microsoft offers. An analyses and a mapping of the individual characteristics of the products and the requirements is defined in this thesis.
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The current level of compliance with the concept of prenatal care from the perspective of pregnant women
URBÁNKOVÁ, Martina
This bachelor thesis deals with a current level of abidance of the prenatal care conception from the point of view of pregnant women. Theoretical part is divided into four main chapters. The first chapter is focused on the history of midwifery and prenatal care. The second one describes prenatal care in general, organisations, goals, antenatal preparation by the midwife, pregnancy card and a role of the midwife in the prenatal consulting. The third chapter deals with pregnancy diagnosis and the last one concerns the examination of pregnant women in each trimester. Practical part of the thesis points out a comparison of a current level of abiding the prenatal care conception in České Budějovice and Benešov region.Three goals were set prior to writting this thesis. The first goal was to find out the abidance of the prenatal care conception from the point of view of pregnant women. The second goal was to find out, whether pregnant women understand the importance of all the examinations carried out during prenatal consultings. The third goal was to find our and compare the level of abiding the prenatal care conception in České Budějovice and Benešov region. Three questions arose from these three goals: What is the level of abiding the prenatal care conception? What examinations are being carried out during prenatal consultings? What is the difference between abiding the prenatal care conception in České Budějovice and Benešov region?The qualitative method had been used to conduct the survey.Answers to the questions from the survey were searched during the acquisition and processing of the semi-structured interviews. Conclusion of the survey is, that all the pregnant women are satisfied with the current conception of the prenatal care. This way of timing of visit intervals in prenatal consultings seems right to them, important and necessary, not only for a control over themselves, but also for a control over the fetus. The level of the conception of prenatal care is good, satisfactory for all the mothers. Surprising result was, that half of the mothers is unable to describe and elaborate what examinations they went through in prenatal consulting, when it was and what was its purpose. Especially when only two respondents complained about insufficient awareness from the physician about performed examinations. As the most common examinations were stated: blood pressure, urine, weight, CTG scan and vaginal examinations. Based on the conducted survey I have managed to find out that the frequency of regular visits in prenatal consulting, according to CGOS recommendations in České Budějovice was abode only in two cases. This fact was not confirmed by any respondent from the Benešov region. Schedule of the regular visits depends on physician´s consideration. Another difference was not discovered. Current level of abidance of the prenatal care conception in Benešov region is similar to České Budějovice.The thesis may serve as an informational material in prenatal consultings in the future, especially for pregnant women, who want to know the progress of prenatal care.
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Awareness of vegetarians about deficit of certain nutritions in vegetarian diet and about ways to compensate them
VORLÍČKOVÁ, Markéta
This thesis deals with vegetarians' awareness of the lack of specific nutritional substances in the vegetarian diet and their compensation. The aim is to explore whether vegetarians have sufficient information about the nutritional composition of their diet, whether they are interested in researching this information and whether the information is available and sufficient. I also looked at whether people with this eating preference are preventing potential nutritional deficits and subsequent health problems. There were designated the following three research questions: "How often and where do most vegetarians get information about a meatless diet?", "How can you achieve a full fledged vegetarian diet?" and "What is vegan awareness on the potential deficiencies in their diet, and how do they try avoid these?". In the theoretical part I deal with vegetarianism as a lifestyle, vegetarianism and its subgroups, the reasons for going to this way of life, the composition of a vegetarian diet with a focus on nutrients that may be deficient, vegetarianism and its impact on the body at different stages of development and ultimately the impact of a meatless diet on human health. The practical section is an elaborated form of qualitative research, specifically I chose semi-structured interviews. I collected the data from the interviews. The interview contained fourteen or fifteen questions, and women were also questioned about the pregnancy period. I asked questions regarding the issue of vegetarianism, veganism or raw foodism. I was interested in individual eating habits, nutrition knowledge and personal experience of the respondents. The data was collected between January 2014 and March 2014. The research group consisted of 6 respondents. The group was made up of women and men in the age range from 20 to 30 years. Among respondents there were vegetarians, vegans and raw foodists and the period of time in which they have been eating this way is different for every person. All respondents were familiar with the purpose of providing an interview and agreed to the publication. The interviews I conducted in person and were recorded on a dictaphone mobile phone. The results of my work show that most respondents do research about a meatless diet. They logically state that most intensive research took place while they were switching their eating habits, but now they still keep finding out new information. The most commonly used way was the Internet, specifically through websites such as www.soucitne.cz or www.vegan-fighter.com. Other sources used are books, articles, studies, and information received from friends or family members. The respondents argue that because the information was obtained this way, their diet is balanced. They are often trying to maintain diversity in their diet, for example, consume legumes and combine various foods such as legumes with cereals. Raw foodists state that they often consume sprouts, seeds, nuts, and of course a large amount of raw vegetables and fruits. All Vegans and Raw foodists know about the possible lack of vitamin B12, and some for this reason, use supplements. Also mentioned are supplements or protein complex supplements designed specifically for vegans.
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Comparison of old and current cine lenses in combination with modern digital camera
Jakubec, František ; HOJDA, Petr (advisor) ; MIKŠ, Antonín (referee)
A Director of Photography has many artistic possibilities when working with cine lenses. Today, we can produce many optical effects in digital post-productions; however, working with lenses and with their defects (aberrations) is very interesting when you use them with modern recording technology. This thesis examines a small part of these issues. The theoretical part is about general qualities of optical systems, their aberrations, and measurement of picture quality. Furthermore, there is also a description of the movie camera and lenses, which were used for testing. The analytical part is about analyzing real footage from tests.
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Vegetation succession and soil development on heaps from brown coal mining
Veselý, Martin ; Chuman, Tomáš (advisor) ; Šefrna, Luděk (referee)
Plant diversity and selected soil parameters from 36 sites placed on brown coal mining spoil heaps were analyzed and compared. Spontaneously revegetated or technically reclaimed spoil heaps of different age were situated in brown coal mining district of eastern Most basin in north western part of the Czech republic. Samples were sorted in groups according to vegetation growth and type. Plant diversity increases during succession development and is higher on non-reclaimed sites than reclaimed sites, where diversity decreases with time. Soil bulk density decreases during succession on all sites. Soil pH is decreasing too and this process is faster on spontaneously revegetated sites. Old successional forest type sites have very acid soils probably due to properties of original mineral matrix. Thickness of organic horizon and A horizon is increasing during succession and they appear in mid-aged sites first. Content of soil organic carbon is increasing continuously. Non-reclaimed sites reach higher contents, mainly because of higher content of fossil organic matter in spoil mineral matrix. Keywords: vegetation succession, soil development, reclamation, brown coal mining heaps
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Sorption and Stabilization of Metals/Mettalloids by Innovative Synthesized Sorbent Amochar.
Ouředníček, P. ; Trakal, L. ; Komárek, M. ; Pohořelý, Michael
Remediation of contaminated soil which is based on stabilization and immobilization of potential\nhazardous substance by sorption materials has been studied intensively nowadays. Biochar – activated organic carbon belongs to this group of stabilizing agents which can adsorb wide range of contaminants, including metals/metalloids. Surface area of the biochars is quite large in general and functional groups (e.g. COO–) can form chelates or alkaline elements on the surface, which is represented by cation exchange capacity. Altogether with the high pH values (7.00 – 10.00), biochars are quite effective sorbents and can adsorb metals/metalloids from the solution (ground water), especially in acidic soils (in the environments affected by intensive mining activities). Sorption\neffectiveness can be increased (especially for As (V) or Cr (VI) sorption) by modification of biochar by various types of secondary oxides. Innovative sorbent AMOchar (AMO + biochar) has been synthesized currently. The product was prepared by adding of biochar to the reaction solution during amorphous manganese oxide (AMO) synthesis. The AMOchar was formed mainly by Mn-oxalates which had coated surface of the pristine biochar. AMOchar composite was able to remove significantly higher amounts of various metal(loid)s from the solution despite the rather high pH of the material. Sorption effectiveness was high not only in case of Pb (II) sorption (almost 99%), and Cd(II) (51.2%), but also a very high amount of As(V), 91.4%. Additionally, both AMOchar composite was able to reduce Mn leaching. This can avoid potential post-contamination caused by the dissolution of less stable Mn-oxalates as observed in the pure AMO.
Fulltext: content.csg - PDF Plný tet: SKMBT_22316111113040 - PDF
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