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The development of Leishmania in the insect vector of the genus Phlebotomus
Kvapilová, Kateřina ; Volf, Petr (advisor) ; Bilej, Martin (referee)
The work was focused on the study of various aspects of development of Leishmania in vectors of genus Phlebotomus and can be divided into two main parts. In the first part, we studied the competition of Leishmania major and L. turanica during their development in the sand flies who are natural vectors of L. major using the experimental co-infections of fluorescently marked promastigots of these two species. While both leishmania species developed similar in the intestine of Phlebotomus papatasi, L. turanica prevailed in P. duboscqi in the late stages of infection. The fluorescent marking of Leishmania should allow us also to study possible genetic exchange between species at different stages of Leishmania infection (2nd, 9th and 14th day after the infective feeding). Using the flow cytometry (FACS) we have repeatedly identified dozens of objects emitting red and green signals in the intestinal homogenates of co-infected sand flies, however further analysis with confocal microscope disproved these objects as the hybrid promastigots of Leishmania. In the second part of this thesis we investigated the role of L. major genes HASP and SHERP, which lies on the locus LmcDNA16 and are expressed exclusively in metacyclics. We used mutant lines KO (lacking the locus LmcDNA16) and HASPB (KO line with gene...
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The importance of the German economy in the global economy
Škoríková, Romana ; Šaroch, Stanislav (advisor) ; Sankot, Ondřej (referee)
The aim of this work is to characterize the importance of the German economy in the global economy on the basis of SWOT analysis. The work is composed as an analysis of the strengths and weaknesses, forthcoming opportunities and threats. The focus is on the role of interconnectedness of country and the state of its economy. The first chapter is about the economic situation in Germany, the second chapter is devoted to position in international trade. The third, and final chapter, I deal with political consequences.
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Analysis of competition in the services market
Shashkova, Tatiana ; Soukupová, Jana (advisor) ; Adámek, Petr (referee)
The thesis deals with the analysis of market competition hairdressing services, concretely with analysis of three salons which provide hairdressing services in the Centre of Prague. Preliminary I present and define key terms that play basic role in the analysis of competition in the chosen field (competition, the specifics of hairdressing market, the specifics of consumer behavior). To make my own research I elected standards that influence customers when they choose a hairdressing salon, and I created questionnaire. During valuation I commented point gains. Total score were used to to evaluate the competitive position of hairdressing salons in the market.
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Analysis of Financing Research and Development in the Czech Republic
Vacek, Jan ; Hnilica, Jiří (advisor) ; Sieber, Patrik (referee)
The topic of this bachelor Thesis is an Analysis of Financing Research and Development in the Czech Republic. Research and Development (R&D) plays an important role in improving the competitive ability of the Czech Republic, for it constitutes a source for increasing productivity, economic growth and employment. The results of R&D lead to creation of new products and services, which in return increases the quality of life of the Czech citizens. This bachelor thesis focuses on analysis of financing R&D in the Czech Republic. The objective is to find out who finances R&D in the Czech Republic, where do these funds go to, and what they are used on. This thesis also focuses on R&D employees and R&D departments in which they work. The bachelor thesis is concluded by comparison between the R&D data for Czech Republic and other countries form the Europian Union.
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Role of UNHCR in case of asylum seekers, refugees and stateless persons – the case study of Turkey
Unzeitigová, Klára ; Novotná, Markéta (advisor) ; Kuchařová, Alžběta (referee)
Inspired by the Kratochvíl, Cibulková and Beník´s model of actorness, the thesis explores the framing power of UNHCR. The critical discourse analysis introduced by Norman Fairclough is applied in order to analyze the textual, contextual and sociocultural dimension of the UNHCR framing power. The case of Turkey was chosen for this study due to the current situation and the fact that Turkey is one of the biggest hosting country in the refugees´ crisis. Through the critical discourse analysis, the thesis explores whether UNHCR is considered as a framing actor in the case of asylum seekers, refugees and stateless people in Turkey. The analysis shows that UNHCR is globally respected and takes important part in the issue. However, not all of the international organizations refer to UNHCR as the lawmaker and the initiator of debates in the issues mentioned above.
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Výběr a implementace open source nástroje pro řízení portfolia projektů
Marek, Jan ; Chlapek, Dušan (advisor) ; Kučera, Jan (referee)
Methods and ways of implementation of changes and innovations in companies through project management are in today's society very well established. There exists methodologies, techniques and tools for the management of individual projects. However in a role of project manager, I very often faced the fact that companies are performing the project portfolio management in very intuitive way. This in itself leads to failed and prematurely terminated projects, initiation of the wrong projects or realisation of correct projects, but at the wrong time. Very often I have also recognized, that there is lack of awareness of fact, that there are also Open Source applications that can help with the organization's portfolio. This thesis deals with the definition of requirements, search and selection of proper OSS application and subsequent implementation. In the first part of this thesis I prepare a theoretical framework about PPM, and on that basis then identify and verify a set of requirements for selection of right OSS PPM application. The next section of thesis describes searching for suitable applications from a variety of sources, assessment against the requirements and the final selection. In the last part the implementation project is drafted, which aims to serve other colleagues in project management as one of the possible implementation paths. The outputs are then continuously confronted with experts in matter of projects and project management in the field of IT in order to maximize symbiosis between theory and real life experiences. Virtually every project management methodology defines the collection of already-proven techniques, best practices or lessons learned from the previous similar implementations. The main contribution of this thesis I see in fact that it contains not only the design of the project implementation, but also describes a logical path, what leads to the result. Therefore this thesis could be used as a base or discussed best practice, when a project of implementation of PPM application in place.
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Supported Emplayment from the Perspective of Emplayers
MYŠÁKOVÁ, Hana
The philosophy of supported employment in the Czech Republic has been developing for over than twenty years. Nevertheless this service is still neglected and only a few non-profit organizations put it into practice. The service is not legislatively based and also the society does not have a clear opinion on employment of people with disabilities. Current labour market situation is not advantageous at all. Therefore some support of disabled people looking for a job is becoming more necessary and required. The employers are an important part of the whole problems, because their attitude to the idea of supported employment plays a significant role. The bachelor's thesis "Supported Employment from the Perspective of Employers" has pretentions to look into supported employment from the employers' point of view. They are not active initiators of the service, but they are important participants for its successful realization. The theoretical part of the thesis tries to describe the basic problems. It covers the service of supported employment from historical development to its importance. It focuses on special characteristics of disabilities and their social impact on human beings. The closing part is dedicated to working opportunities for people with disabilities in the circumstances of current legislative and economic situation. The practical part is written in the form of a qualitative research. The method of questioning and semi-structured interviews were used for the purposes of the research. Eight people were chosen intentionally for the survey. The data gained from the interviews were analysed with the method of open coding. The main aim of the thesis was to map out empoyers' experience with the service of supported employment. Motivation of employers to put this service into practice was the next part of the research. The question was: "What reasons for realization of supported employment do predominate by employers?" The research showed the most frequent facts, which predestine the integration possibility of disabled people in open labour market fundamentally. After the interviews and answering the questions of the research, it was possible to describe the employers' experience with the supported employment service and also their main incentives for its realization. It was found out that employers' experience with the supported employment service is quite limited. Employers often get to know it through the agency for supported employment, which is not only the initiator of cooperation, but it also plays an important role when a disabled person is being employed. An important point of successful employment is often how the emploeyer is satisfied with the agency. The employment of disabled people often brings certain risks connected with the limits of disabilities. Employers, who enable disabled people to work, are aware of that. The impulse for employment can vary from financial advantages, company philantrophy to personal solidarity of the employer. Social responsibility of the company is becoming a trend, that many employers start to know. But it can be presumed to influence the employment of more people with disabilities and at the same time the looking up the supported employment service by the employers. This bachelor's thesis can be contributive to understanding the importance of employment for disabled people's living. It brings information about the employers' experience with the realization of supported employment and motivation to the realization. It can also help to uncover integration obstacles of people with disabilities in open labour market.
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Study of the composition and organization of cytochrome P450 system by covalent crosslinking
Koberová, Monika ; Hodek, Petr (advisor) ; Hansíková, Hana (referee)
The system of mixed function oxygenase (MFO system) participates in significant roles in the metabolism of endogenous compounds and xenobiotics. This system contains cytochrome P450, NADPH:cytochrome P450 reductase, and also there are assigned NADH: cytochrome b5 reductase and cytochrome b5. It was proved that cytochrome b5 can stimulate or inhibit cytochrome P450 (CYP)-dependent reactions and even change the ratio of resulting metabolites. The mechanism of cytochrome b5 action has not been fully elucidated yet. Elucidation of protein-protein interactions in MFO system and determination of topology of this system could explain the mechanism of cyt b5 action. The covalent cross- linking technique is suitable method for identifying protein-protein interactions within the membrane. Cytochrome b5 contains 3 methionines and in 2 cases the methionines are localized in a short hydrophobic C-terminal membrane anchor. Interactions with cytochrome P450 in the membrane environment can be identified by substitution of two methionine for photoactivatable analogue of methionine (photo-methionine) and subsequent photoactivation. This work is focused on expression and isolation of photo-cytochrome b5 (photo- cyt b5), cytochrome b5 analogue with incorporated photo-methionine. Conditions for photo-methionine...
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Miřenka Čechová
Janečková, Kateřina ; PETIŠKOVÁ, Ladislava (advisor) ; SOPROVÁ, Jana (referee)
Monographic work focuses on artistic creation Miřenka Čechová, one of the
the leading personalities of our contemporary stagecraft. Her personal and
artistic development started when she began studying dance and continued to
mimic and physical theater. Material presents her contribution to
contemporary Czech scene physical theater and refers to her international
success. Work observes her role like co-founder of physical theaters Theatro
Pantomissimo and Spitfire Company and to presents the work of these
physical theaters and reveals the working and teaching methods in the
preparation of copyright performances.
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