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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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Retargetable Analysis of Machine Code
Křoustek, Jakub ; Janoušek, Jan (referee) ; Návrat,, Pavol (referee) ; Kolář, Dušan (advisor)
Analýza softwaru je metodologie, jejímž účelem je analyzovat chování daného programu. Jednotlivé metody této analýzy je možné využít i v dalších oborech, jako je zpětné inženýrství, migrace kódu apod. V této práci se zaměříme na analýzu strojového kódu, na zjištění nedostatků existujících metod a na návrh metod nových, které umožní rychlou a přesnou rekonfigurovatelnou analýzu kódu (tj. budou nezávislé na konkrétní cílové platformě). Zkoumány budou dva typy analýz - dynamická (tj. analýza za běhu aplikace) a statická (tj. analýza aplikace bez jejího spuštění). Přínos této práce v rámci dynamické analýzy je realizován jako rekonfigurovatelný ladicí nástroj a dále jako dva typy tzv. rekonfigurovatelného translátovaného simulátoru. Přínos v rámci statické analýzy spočívá v navržení a implementování rekonfigurovatelného zpětného překladače, který slouží pro transformaci strojového kódu zpět do vysokoúrovňové reprezentace. Všechny tyto nástroje jsou založeny na nových metodách navržených autorem této práce. Na základě experimentálních výsledků a ohlasů od uživatelů je možné usuzovat, že tyto nástroje jsou plně srovnatelné s existujícími (komerčními) nástroji a nezřídka dosahují i lepších výsledků.
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Digital circuits test optimization by multifunctional components
Stareček, Lukáš ; Gramatová, Elena (referee) ; Kubátová, Hana (referee) ; Kotásek, Zdeněk (advisor)
This thesis deals with the possibilities of digital circuit test optimization using multifunctional logic gates. The most important part of this thesis is the explanation of the optimization principle, which is also described by a formal mathematical apparatus. Based on this apparatus, the work presents several options. The optimization of testability analogous to inserting test points and simple methodology based on SCOAP is shown. The focus of work is a methodology created to optimize circuit tests. It was implemented in the form of software tools. Presented in this work are the results of using these tools to reduce the test vectors volume while maintaining fault coverage on various circuits, including circuits from the ISCAS 85 test set. Part of the work is devoted to the various principles and technology of creating multifunctional logic gates. Some selected gates of these technologies are subject to simulations of electronic properties in SPICE. Based on the principles of presented methodology and results of multifunctional gates simulations, analysis of various problems such as validity of the modified circuit test and the suitability of each multifunctional gate technology for the methodology was also made. The results of analysis and experiments confirm it is possible for the multifunctional logic gate to optimize circuit diagnostic properties in such a way that has achieved the required circuit test parameter modification with minimum impact on the quality and credibility of these tests.
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Grammars with Restricted Derivation Trees
Koutný, Jiří ; Janoušek, Jan (referee) ; Vojnar, Tomáš (referee) ; Meduna, Alexandr (advisor)
V této disertační práci jsou studovány teoretické vlastnosti gramatik s omezenými derivačními stromy. Po uvedení současného stavu poznání v této oblasti je výzkum zaměřen na tři základní typy omezení derivačních stromů. Nejprve je představeno zcela nové téma, které je založeno na omezení řezů a je zkoumána vyjadřovací síla takto omezené gramatiky. Poté je zkoumáno několik nových vlastností omezení kladeného na cestu derivačních stromů. Zejména je studován vliv vymazávacích pravidel na vyjadřovací sílu gramatik s omezenou cestou a pro tyto gramatiky jsou zavedeny dvě normální formy. Následně je popsána nová souvislost mezi gramatikami s omezenou cestou a některými pseudouzly. Dále je prezentován protiargument k vyjadřovací síle tohoto modelu, která byla dosud považována za dobře známou vlastnost. Nakonec je zavedeno zobecnění modelu s omezenou cestou na ne jednu, ale několik cest. Tento model je následně studován zejména z hlediska vlastností vkládání, uzávěrových vlastností a vlastností syntaktické analýzy.
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Navigation of mobile robots
Rozman, Jaroslav ; Matoušek,, Václav (referee) ; Šolc, František (referee) ; Zbořil, František (advisor)
Mobile robotics has been very discussed and wide spread topic recently. This due to the development in the computer technology that allows us to create better and more sophisticated robots. The goal of this effort is to create robots that will be able to autonomously move in the chosen environment. To achieve this goal, it is necessary for the robot to create the map of its environment, where the motion planning will occur. Nowadays, the probabilistic algorithms based on the SLAM algorithm are considered standard in the mapping in these times. This Phd. thesis deals with the proposal of the motion planning of the robot with stereocamera placed on the pan-and-tilt unit. The motion planning is designed with regard to the use of algorithms, which will look for the significant features in the pair of the images. With the use of the triangulation the map, or a model will be created. The benefits of this work can be divided into three parts. In the first one the way of marking the free area, where the robot will plan its motion, is described. The second part describes the motion planning of the robot in this free area. It takes into account the properties of the SLAM algorithm and it tries to plan the exploration in order to create the most precise map. The motion of the pan-and-tilt unit is described in the third part. It takes advantage of the fact that the robot can observe places that are in the different directions than the robot moves. This allows us to observe much bigger space without losing the information about the precision of the movements.
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Multimedia Data Processing in Heterogeneous Distributed Environment
Kajan, Rudolf ; Ferko,, Andrej (referee) ; Míkovec, Zdeněk (referee) ; Herout, Adam (advisor)
Pervasive computing sa zameriava odstránenie zložitostí pri interakcii s výpočtovou technikou a zvýšenie efektivity pri jej každodennom používaní. Ale i po viac ako 15 rokoch od sformulovania hlavných cieľov Pervasive computingu existujú aspekty interakcie ktoré stále nie sú súčasťou užívateľskej skúsenosti s dnešnou technológiou. Bezproblémová integrácia s prostredím vedúca k technologickej neviditeľnosti, alebo interakcia naprieč rôznymi zariadeniami predstavujú stále veľkú výzvu. Hlavným cieľom tejto práce je prispieť k tomu, aby sa ciele Pervasive computingu priblížili k realizovaniu tým, že predstavíme spôsob intuitívneho zdieľania informácií medzi osobným a verejne umiestneným zariadením. Predstavili sme tri interakčné techniky, ktoré podporujú intuitívnu výmenu obsahu medzi osobným zariadením a zdieľaným displejom. Tieto techniky sú založené na prenose videa, rozšírenej realite a analýze pohľadových dát. Okrem interakčných techník sme tiež predstavili mechanizmus pre získavanie, prenos a rekonštrukciu aplikačného stavu na cieľovom zariadení.
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TIB AV-Portal: A Reliable Infrastructure for Scientific Videos
Plank, Margret
The German National Library of Science and Technology provides a web-based platform for quality-tested scientific videos including computer visualizations, experiments, interviews as well as recordings of lectures and conferences. Key feature of the portal is the use of automated video and semantic analyses, which enables pinpoint and cross lingual search on a segment level. All videos are assigned by Digital Object Identifi ers (DOI). As a result videos can be published in a scientifically sound way and be linked via DOIs to other research work like the corresponding article.
Fulltext: idr-1033_4 -  PDF
Slides: idr-1033_2 - PDF; idr-1033_3 - PDF
Video: idr-1033_1 -  MP4
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Mechanochemical Preparation of Alumina-Ceria
Jirátová, Květa ; Spojakina, A. ; Tyuliev, G. ; Balabánová, Jana ; Kaluža, Luděk ; Palcheva, R.
Ceria containing catalysts play an essential role in heterogeneous catalytic processes. However, ceria shows poor thermal stability and low specific surface area and therefore, many studies have been done to improve its properties by combination with other oxides. Alumina-ceria is substantial component of the three ways catalysts, due to the ceria ability to function as the buffer of oxygen and to enhance the oxygen storage capacity of the catalysts. Ceria in these catalysts also functions as structural promoting component, increasing alumina stability towards thermal sintering. Promising method of oxides preparation, very interesting and simple but not sufficiently studied yet is a mechanochemical synthesis. Here we report on the synthesis of nano-sized alumina, ceria and ceria-alumina of various compositions by a wet solid phase mechanochemical reaction of hydrous aluminum, and/or cerium nitrate with ammonium bicarbonate after addition of a small amount of water. The aim of this contribution is to study processes being in progress during synthesis of the mixed oxides, interaction between components and their mutual effect on the properties of resulting products. The phase evolution during mechanical milling and the subsequent heat treatment of precursors were studied by X-ray diffraction, DTA/TG, H2-TPR, NH3-TPD, CO2-TPD, N2 adsorption at -195°C, IR, and XPS spectroscopy. Alumina and mixtures of alumina with different quantities of CeO2 (1- 18 wt. %) were synthesized by mechanochemical method from aluminum nitrate, cerium nitrate and ammonia bicarbonate.
Fulltext: content.csg - PDF
Plný tet: SKMBT_C22015083108560 - PDF
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Role and competences of the nurse in compared with Czech Republic and Namibia
KLYNCYPAROVÁ, Hana
Theoretical grounds The nursing profession among the professions and mission. Job nurse is one of very important professions. Nurse is an independent worker who has an irreplaceable role in the provision of care. In practice, certain roles, such as the role of a caregiver, the educationalist, communicator, the role of the lawyer, consultant, person, who brings the change, manager and researcher. These roles are constantly interacting and are interconnected. Health care has multidisciplinary and multiprofessional character. Although each of these professions contribute to care in a unique way, all share certain skills that are necessary to ensure the quality of care. Over the years there have been increasing professional skills and increase competencies. Expanded the range of services for which they previously did not have the competence or sisters had a written authorization of a doctor. This work is made up of theoretical and empirical part. The theoretical part provides an overview and information on the field of nursing and its history, dealing with the care system and education system in the Czech Republic and Namibia. The following list of findings roles and their importance in the nursing profession. The last part deals with the competencies of nurses in both countries. The aim of the thesis: The aim of the study was to determine the roles and responsibilities of nurses in the Czech Republic and Namibia. To to obtain this information were chosen research question what role nurses advocate in the Czech Republic and Namibia, and what are nurses in the Czech Republic and Namibia competence. Methods: To ensure the necessary data and information was chosen qualitative research through deep interviews. Nurses who were interviewed were from a hospital in the Czech Republic and nurses from hospitals in Namibia Keetmanshoop. All interviews were recorded on a Dictaphone andwritten down on the paper. Results: Recorded interviews were analyzed after writing down, we were using paper and pencil methods. Dialogs were openly coded. The analysis of these data arose following categories: the role of caregivers, the role of the teacher, communicator, the lawyer's role, the role of counselor, role of person who brings the change, the role of leader, manager's role, the role of researcher and categories of competence. Individual categories were assigned to subcategories. All informationwere are illustrated by clear diagrams of the individual categories. Conclusion: The Research shows that nurses have a general maintenance of the roles and competencies, actually both of sides, Namibia and Czech Republic also. both groups were mostly able to define roles in nursing care. Surprisingly czech nurses most failed in the role of the Advocate, while African colleague have proven to be very informed. Research also showed negative effect of paperwork and administration in czech system. Nurses having feeling they do not provide active care as they would be able to. Czech nurses seems also low initiative and ambitious, Czech nurses do not desire for increasing competence on the other hand African nurses, who have more competencies, want still more, than they already have.
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The reflection of František Bakule's work in a relation to a current special educational practice
BALÍKOVÁ, Tereza
František Bakule was a significant Czech teacher during the first half of the 20th century. He was known as the first director of the "Jedlicka Institute in Prague" as well as the founder of so-called "Bakule´s Institute" as well as one of the few representatives of the "Czech alternative approach towards education" which is very well recognized all around the world. His legacy and teachings, however, are currently not being developed on the systematic level and neither they are being put into practise by Czech professional mainstream educational community. We also need to mention that is not used by even the stream of special education, in contrast to many foreign alternative pedagogical trends, which are gaining popularity and are generally applied with success. The aim of this thesis is therefore to analyse the work of Frantisek Bakule through the prism of contemporary school educational environment with emphasis on the education of students with disabilities. The first chapter is devoted to outlining the life journey of Frantisek Bakule, particularly focusing on the major milestones that influenced the formation of his personality, his ideas, inspiration and motivation which was reflected in his teachings and then in his very own coherent concept of education. To be specific, we are introduced to his family background and a significant part is then devoted to description of Frantisek´s experience as a teacher, including stages of operating in Jedlicka´s Institute and in his own "Bakule´s institute". The second part is then focused solely on Bakule´s specific educational concept. First, the core principles of the concept are defined. Later parts then talk about Bakule´s original curriculum and a his proposal of an ideal classroom environment. The text also contains a detailed description of how Bakule approached several subjects, which were at the core of his teaching concept, namely "Work activities", "Music education" and "Arts". Original teaching practices are analysed by using current teaching methodology and terminology. Due to the identified aim and goals of the thesis, the third chapter analyses the current concept of education of the above, according to Bakule´s core areas and principles. The text is designed specifically to be subsequently reflecting the work of Frantisek Bakule from the perspective of today's teaching methods, namely the methods of special education. The idea is to define common points and possible differences and uncover hidden inspirational potential, which is then the main content of the fourth and final chapter. Based on the analysis and all its subsequent reflections, the final chapter tells us that although Bakule´s educational goals don´t differ too significantly from the current concept embedded in the relevant framework of educational programs, to achieve such goals we are currently using very different techniques and methods in comparison to Bakule´s ways. Even though Bakule himself tested and verified all of his concepts in practice, which can be very simply described as "do not teach students about life and work, but through life and work", and these concepts also have been proven correct by various subsequent results of psychological researches, the current system still doesn´t draw from them too much. From all the above it is quite obvious that the legacy of life´s work of Frantisek Bakule is currently not utilized nor it is being appreciated by our professional pedagogical community. On the other hand, we need to note that it may also be due to the level of fragmentation in which his concept and materials were preserved to the present days.
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