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Růst Mycobacterium smegmatis na agarovém médiu a agarovém médiu pokrytém celofánovou folií - morfologická a proteomová studie
Ramaniuk, Volha ; Weiser, Jaroslav (advisor) ; Beranová, Jana (referee)
Biofilm formation is one of the most common bacterial survival strategies. Majority of bacterial species are able to form these three-dimensional structures, including pathogens like Mycobacterium tuberculosis. Representatives of Mycobacterium genus widely occur in the nature, although they can cause serious problems when they appear in medical equipment and artificial replacements of the human body. Non-pathogenic Mycobacterium smegmatis mc2 155 was used as a model organism in our experiments. We investigated morphology of the three- and six-day-old colonies (in fact biofilms) on agar and agar covered with cellophane using Stereo microscope and Scanning Electron Microscope. We found that a type of surface as well as a carbon source has a great influence on the morphology of the M. smegmatis colonies. We isolated proteomes from the agar and cellophane cultures and from planktonic culture. Two-dimensional electrophoresis was used as the main proteomic method. Proteomic data were analyzed using PDQuest software. Then the sets of proteins detected by qualitative and quantitative analyses were compared using Venn diagrams. As a result, we recognized 7 unique proteins that might be specific for recognition and adhesion of bacteria to the cellophane, no unique protein in agar proteome and 46 unique...
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Usage of unstructured data in Business Intelligence
Rakhmanova, Malika ; Šperková, Lucie (advisor) ; Karkošková, Soňa (referee)
The aim of the thesis is to identify the main trends that are occurring in the market of Business Intelligence and related to unstructured data, to describe the possibilities for integrating unstructured data, to clarify what the impact on the company have the results that can be obtained using these solutions and how generally incorporate an analysis of unstructured data into BI. Another aim is to show the current situation of processing unstructured data on the example of BI system. The thesis is divided into several parts. First part is describing of the Business Intelligence area and the basic components of Business Intelligence, as well as identifying market trends. Then, there is the next part: separating the data into structured and unstructured. Here is the part about how you can access and analyse unstructured data and what is their place in BI systems. This is the end of a block of unstructured data and the beginning of a description of the enhanced version of BI. Finally, the current market situation and BI tools, which include unstructured data, are introduced. This section provides an overview of how BI tools approach to analyse unstructured data. Existed literature, professional and freely available Internet resources are used for writing the work. The purpose is to serve as a source of information for quickly orienting in the current situation, to serve as a guide to the world of BI solutions and to show potential users what are the options and functionality of these BI solutions.
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Analysis of the Motivation and Satisfaction of Employees in a firm
Gonta, Natalya ; Dvořáková, Zuzana (advisor) ; Odvárková, Markéta (referee)
The aim of this study is to analyze the motivation and satisfaction of employees at British American Tobacco Ltd. The bachelor thesis consists of two parts - theoretical and practical. The first part describes the concepts and sources of motivation, which affect the performance of employees in the company. The second part describes the aim of the research, its progress and evaluation results in respect of which was carried out to analyze the way of motivation and satisfaction of employees in the company. For the following analysis methods were used questionnaire and a personal unstructured interview. The result of the thesis is mentioned proposal for improving the motivation of employees, which has potential and could be implemented in the company.
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The Problems of Acceptability of Competence of a Specialist Nurse (ARIC) to Male Urethral Catheterization
SOUKUPOVÁ, Pavla
The passing of the act on non-physician medical professions and the decree on activities of healthcare workers has brought numerous changes that are also linked to changes of nurse competences. Aim 1: To find out whether nurses with ARIC specialization perform male urethral catheterization. Aim 2: To find out whether nurses with ARIC specialization are interested in improvement of their knowledge of male urethral catheterization. Aim 3: To find out whether nurses with ARIC specialization have sufficient practical skills in male urethral catheterization. Aim 1: To find out what opinion physicians have on male urethral catheterization performed by a nurse specialized in ARIC. Aim 2: To find out whether physicians let nurses specialized in ARIC perform male urethral catheterization. The research part of the thesis was based on quantitative and qualitative research. The research results will be provided to head nurses in the hospital where the research was performed. The results might be useful for preparation of a specialization course focused on male urethral catheterization not only for nurses specialized in ARIC, but also for nurses that are interested in performing the procedure in the future, particularly as it is known that no such a course exists in the South Bohemia. The results might also be used as an input to further research.
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World Trade in Crude Oil
Benešová, Nela ; Čajka, Radek (advisor) ; Bolotov, Ilya (referee)
This master´s thesis deals with trade with crude oil and its impact on the world economy. Everyone, who wants to start to trade with crude oil, should find out basic terminology and information about crude oil that is characterized in the first chapter. The hunt for oil started many years ago, therefore the history is also mentioned in this master´s thesis. The second chapter focuses on the world trade with crude oil. Territorial structure and trade flows are described in this part of the master´s thesis. Nevertheless, the crude oil also has an impact on the world economy and economies of single states. The third chapter analyses states, where the inhabitants live beyond means of people from other countries but these states are so depended on crude oil that if this trade stopped, these countries would go bankrupt. However, there are also exceptions. Even though, there are states that have huge sources of crude oil, they still have to deal with financial problems. The forth chapter characterizes the trade with crude oil of the Czech Republic because it is a net importer of crude oil and the last chapter focuses on new trends in crude oil such as shale oil and trading at a crude oil market.
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The Conclusion of a Contract under the United Nations Convention on Contracts for the International Sale of Goods in Comparison with Czech National Legislation
Krčálová, Kristýna ; Boháček, Martin (advisor) ; Levý, Jan (referee)
This Master's thesis focuses on the United Nations Convention on Contracts for the International Sale of Goods (CISG), better known as the Vienna Convention. The main purpose of this thesis is to evaluate conditions for application of this international treaty and to assess the contracting process under the CISG including the similarities and differences with the Czech national legislation. Finally, it seeks an answer to the question, which of these rules (international or national) are more advantageous for a Czech contractual party with respect to concluding a contract. The thesis is divided into five parts. The first one represents an introduction to the CISG, its origin and the Czech Republic's attitude to it. The second part identifies the conditions for application of the CISG and therefore defines a subject and an object of an international contract of sale under the CISG. Both third and fourth parts gradually introduce two phases of the contracting process (offer and acceptance) including the relevant case-law. The last chapter strives to complete the contracting process with an analysis of the exact moment, when a contract is concluded, as well as with an analysis of a potential battle of the forms and its impact on a contract conclusion.
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The current level of compliance with the concept of prenatal care from the perspective of pregnant women
URBÁNKOVÁ, Martina
This bachelor thesis deals with a current level of abidance of the prenatal care conception from the point of view of pregnant women. Theoretical part is divided into four main chapters. The first chapter is focused on the history of midwifery and prenatal care. The second one describes prenatal care in general, organisations, goals, antenatal preparation by the midwife, pregnancy card and a role of the midwife in the prenatal consulting. The third chapter deals with pregnancy diagnosis and the last one concerns the examination of pregnant women in each trimester. Practical part of the thesis points out a comparison of a current level of abiding the prenatal care conception in České Budějovice and Benešov region.Three goals were set prior to writting this thesis. The first goal was to find out the abidance of the prenatal care conception from the point of view of pregnant women. The second goal was to find out, whether pregnant women understand the importance of all the examinations carried out during prenatal consultings. The third goal was to find our and compare the level of abiding the prenatal care conception in České Budějovice and Benešov region. Three questions arose from these three goals: What is the level of abiding the prenatal care conception? What examinations are being carried out during prenatal consultings? What is the difference between abiding the prenatal care conception in České Budějovice and Benešov region?The qualitative method had been used to conduct the survey.Answers to the questions from the survey were searched during the acquisition and processing of the semi-structured interviews. Conclusion of the survey is, that all the pregnant women are satisfied with the current conception of the prenatal care. This way of timing of visit intervals in prenatal consultings seems right to them, important and necessary, not only for a control over themselves, but also for a control over the fetus. The level of the conception of prenatal care is good, satisfactory for all the mothers. Surprising result was, that half of the mothers is unable to describe and elaborate what examinations they went through in prenatal consulting, when it was and what was its purpose. Especially when only two respondents complained about insufficient awareness from the physician about performed examinations. As the most common examinations were stated: blood pressure, urine, weight, CTG scan and vaginal examinations. Based on the conducted survey I have managed to find out that the frequency of regular visits in prenatal consulting, according to CGOS recommendations in České Budějovice was abode only in two cases. This fact was not confirmed by any respondent from the Benešov region. Schedule of the regular visits depends on physician´s consideration. Another difference was not discovered. Current level of abidance of the prenatal care conception in Benešov region is similar to České Budějovice.The thesis may serve as an informational material in prenatal consultings in the future, especially for pregnant women, who want to know the progress of prenatal care.
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Comparison of old and current cine lenses in combination with modern digital camera
Jakubec, František ; HOJDA, Petr (advisor) ; MIKŠ, Antonín (referee)
A Director of Photography has many artistic possibilities when working with cine lenses. Today, we can produce many optical effects in digital post-productions; however, working with lenses and with their defects (aberrations) is very interesting when you use them with modern recording technology. This thesis examines a small part of these issues. The theoretical part is about general qualities of optical systems, their aberrations, and measurement of picture quality. Furthermore, there is also a description of the movie camera and lenses, which were used for testing. The analytical part is about analyzing real footage from tests.
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Vegetation succession and soil development on heaps from brown coal mining
Veselý, Martin ; Chuman, Tomáš (advisor) ; Šefrna, Luděk (referee)
Plant diversity and selected soil parameters from 36 sites placed on brown coal mining spoil heaps were analyzed and compared. Spontaneously revegetated or technically reclaimed spoil heaps of different age were situated in brown coal mining district of eastern Most basin in north western part of the Czech republic. Samples were sorted in groups according to vegetation growth and type. Plant diversity increases during succession development and is higher on non-reclaimed sites than reclaimed sites, where diversity decreases with time. Soil bulk density decreases during succession on all sites. Soil pH is decreasing too and this process is faster on spontaneously revegetated sites. Old successional forest type sites have very acid soils probably due to properties of original mineral matrix. Thickness of organic horizon and A horizon is increasing during succession and they appear in mid-aged sites first. Content of soil organic carbon is increasing continuously. Non-reclaimed sites reach higher contents, mainly because of higher content of fossil organic matter in spoil mineral matrix. Keywords: vegetation succession, soil development, reclamation, brown coal mining heaps
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Sorption and Stabilization of Metals/Mettalloids by Innovative Synthesized Sorbent Amochar.
Ouředníček, P. ; Trakal, L. ; Komárek, M. ; Pohořelý, Michael
Remediation of contaminated soil which is based on stabilization and immobilization of potential\nhazardous substance by sorption materials has been studied intensively nowadays. Biochar – activated organic carbon belongs to this group of stabilizing agents which can adsorb wide range of contaminants, including metals/metalloids. Surface area of the biochars is quite large in general and functional groups (e.g. COO–) can form chelates or alkaline elements on the surface, which is represented by cation exchange capacity. Altogether with the high pH values (7.00 – 10.00), biochars are quite effective sorbents and can adsorb metals/metalloids from the solution (ground water), especially in acidic soils (in the environments affected by intensive mining activities). Sorption\neffectiveness can be increased (especially for As (V) or Cr (VI) sorption) by modification of biochar by various types of secondary oxides. Innovative sorbent AMOchar (AMO + biochar) has been synthesized currently. The product was prepared by adding of biochar to the reaction solution during amorphous manganese oxide (AMO) synthesis. The AMOchar was formed mainly by Mn-oxalates which had coated surface of the pristine biochar. AMOchar composite was able to remove significantly higher amounts of various metal(loid)s from the solution despite the rather high pH of the material. Sorption effectiveness was high not only in case of Pb (II) sorption (almost 99%), and Cd(II) (51.2%), but also a very high amount of As(V), 91.4%. Additionally, both AMOchar composite was able to reduce Mn leaching. This can avoid potential post-contamination caused by the dissolution of less stable Mn-oxalates as observed in the pure AMO.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_22316111113040 - PDF
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