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Gas exchange characteristics in relation to genotypes in spinach (Spinacia oleracea) under water stress
Helebrantová, Aneta ; Hnilička, František (advisor) ; Pazderů, Kateřina (referee)
The bachelors dissertation was compiled on theme of: Gas exchange characteristics in relation to genotypes in spinach (Spinacia oleracea) under water stress.
Spinach (Spinacia oleracea) is, similarly as the others leaf vegetables, difficult crop in terms of providing the sufficient level of moisture, therefore the attention is drawn to the varieties of spinach which are resistant to the water stress. Thus the target of cultivation is to find plant which will be resistance to influence of the water stress.
In climabox of department of botanics and physiology was founded experiment with three species of spinach: Misano F1, Monores a Matador. The temperature mode was set to 21 °C during the day and 17 °C during the night. The light mode was set to 16 hours of light and 8 hours of dark per day. Maximum light level in climabox was 800 micromole. The plants were cultivated in 4 recurrences, diagram of experiment is involving two variants: control and stress. The plants in control variant were cultivated in substrate, which was irrigated during the whole time of experiment by 250 ml of water. For the plants in stress variant the supply of water was suspended for 10 days and the substrate was naturally continuously dehydrating. After 10 days the water supply was restored (rehydration) for plants in stress variant, same as level of control variant. The observation was made for the speed of gas exchange (photosynthesis and transpiration) in two-day interval. The speed of gas exchange was measured on leaf area with infrared gasometric gas analyzer Lpro+ (ADC Bioscientific, Hodeson, UK). Measured was conducted in morning hours according. Based on the measured values of photosynthesis and transpiration we calculated water usage effectiveness (WUE).
Based on obtained results is evident that the highest average speed of photosynthesis in control variant was observed at variety of Monores (12,10 micromole CO2.m-2.s-1) and lowest at variety Misano (11,58 micromole CO2.m-2.s-1). The highest average speed of photosynthesis in stress variant was measured at variety of Matador (9,43 micromole CO2.m-2.s-1) and lowest at variety Monores (8,76 micromole CO2.m-2.s-1). There was observed decrease of photosynthesis for each of variety during the water stress.
The highest average values of transpiration were observed at variety Monores (1,97 mmol H2O.m-2.s-1), lowest at variety Matador (1,68 mmol H2O.m-2.s-1). Stressed variety Misano reached level of photosynthesis 1,82 mmol H2O.m-2.s-1. Control variety Matador reached speed of transpiration 1,54 mmol H2O.m-2.s-1 and variety Monores 1,85 mmol H2O.m-2.s-1.Variety Misano was on same level of control variant as stress variant.
The most sensitively reacted variety Monores, which usage of water was 4,43 (10-3). Variety Matador managed the stress well, the usage of water was 5,60 (10-3).
Obtained results confirmed hypothesis of genotype differences depending on water deficit, thus there are differences between gas exchange and WUE in control and stress plants.
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Growing of rapeseed for energy purposes - impacts and context.
Charvát, Michal ; Kuchtová, Perla (advisor) ; Josef, Josef (referee)
Biofuels are generally based on biomass. It may be a waste biomass or biomass grown specifically. It is used as fixed or lump fuel, and also to as liquid and gas. Liquid biofuels are used both for energy purposes, ie to produce heat, electricity generation, but also as a motor fuels. In this thesis goes on biofuel purposefully grown, about oilseed rape and from it made liquid fuel, in this case of RME rapeseed methyl ester, which is a fuel of the first generation.
The European Union expected that the use of biofuel will reduce CO2 emissions by up to 35% further premised that the development of biofuels will be a positive impact on local employment and it the both on direct and indirect. Further help diversify Europe's dependence on oil imports. They were introduced direct support for growers in order to exlarged the area where grew plants intended for biofuel. These direct resistances were abolished in 2010. Similar support was also intended for producers.
Regarding rapeseed, peak in the sown area across the EU was in 2010, when it was sown almost 7 mil. ha, which is almost 3,5 times more than the original plan for the EU. Last year, it was only 6,45 mil. ha, but with higher-yielding rapeseed seeds. Similarly, increasing the sowing areas you can watch in the Czech Republic. Moreover Czech Republic is the fifth producer of rapeseed in the EU. In 2013/14 have been sown most ha rape, total 418,8 th. ha, but production was the highest in the following year due hectare yields, although with reduced the area sown. Yet is rape sown on 14% of cultivated land.
We can not talk about drastically restricting cultivation of rape, even after termination direct aid. First, the price of rape is very decent and steady and almost half designated for industrial processing it is processed on RME. However, the system of cultivation is not good for the soil. Rape in plans crop rotation is ranked every two years mostly, but the best would be once for five years.
The only assumption was confirmed by the EU, CO2 emissions are lower, but is also needed to count with the amount of N2O emissions, which this benefit is reduced. However on employment it had no effect, or rather negative. More jobs in agriculture provides livestock production and the like can be evaluated also process industry. Another problem is the destruction of ecosystems so that could be grown plants suitable for biofuels. It is a matter of developing countries, in our lands is it about the depletion of fertile land to cultivation fuel instead of food. First, it needed more to fertilize and use pesticides. But also in case of crop failure it is revenue designetid for biofuels producers is delivere even at the cost of food price increases.
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Pension reform in the Czech Republic
Štekerová, Nikol ; Pletichová, Dobroslava (advisor) ; Jaroslav, Jaroslav (referee)
Czech Republic faces up to and will even face up to an unfavourable demographic progress from now on. A current situation would become completely intolerable without any transformations. Author reviews every single pillar including a closed down second pillar. In the analytical part there is a suggestion to improve a pension scheme right in several parts. Those most outstanding parts are the motives for a political and economic situation improvement because any reforms success exactly depends on economy performance, a political situation, a demographic risk elimination including elements of birth support and migration and general pension restructure. Several appropriate pension restructure conceptions are suggested. For instance: taking a corporate pension under Swedish pattern which would exclude a question of pre-pension as well. Additionally, a private pension scheme should offer the citizens of the Czech Republic more choices so that everyone could choose the provision for retirement age possibility that is the most appropriate and convenient for them.
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Influence of term of winter rapeseed (Brassica napus) desiccation on yield and seed quality
Rajtmajer, Stanislav ; Bečka, David (advisor) ; Petr, Petr (referee)
Rapeseed is the world's third most important oil plant (the second seminal). It is the most cultivation and most important oil plant in Czech Republic. Winter rapeseed reaches about 85% of the harvest area of oilseeds in the country. The desiccation of oilseed crop is sphere of agricultural engineering, which is still worth discussing. The main problems are how to use the product, what dose and which dates to choose to desiccate. The results of this thesis could help to partially clarify this complex issue.
The aim of the thesis is to observe the effect of different terms oilseed rape desiccation by glyphosate on yield and seed quality.
Small-plot experiments to investigate the influence of the term desiccation of winter rapeseed were established in the years 2013/2014 and 2014/2015 on the lands of the experimental station of the Czech Agricultural University, Faculty of Agronomy Food and Natural Resources at the Červený Újezd.
In the first experimental year 2013/2014 was founded five variants in three replications (including undesiccated control). In the year 2014/2015 was founded seven variants in four replicates (including undesiccated controls). In the first experimental year was the first term desiccation 16. 6. 2014, the second year 8. 6. 2015. Subsequent periods of desiccation were a week apart.
The variety of winter rapeseed used for the experiments was a hybrid variety Rohan. The desiccating agent was used Dominator active substance glyphosate. The dose of
4 l / ha + 200 l H2O.
For all samples, both experimental, years yield was determined, the weight of a thousand seeds and oiliness. For desiccated variants were carried out pre-harvest analysis for the determination of solids in pods. Further, all harvested samples taken for laboratory germination test (Determination of germination) ISTA according to the methodology. The results of the experiment sprouting were statistically analyzed using ANOVA analysis of variance. Differences between mean values were evaluated by Tukey test, the computer program SAS at a significance level of p = 0.05.
Effect of desiccation term influences of winter oilseed rape seed quality in terms of weight and thousands of seeds in terms of oil content. Too early desiccation (46 to 39 days before harvest) HTS reduced by 7-17% and the oil content of 2-4% of the overall average. Oiliness of the observed characters minimum interference term desiccation. Desiccation in the optimum date (17 days before harvest) increased oiliness of 1-6%.
Influence term desiccation oilseed rape also greatly influences seed yield.
Too early desiccation (46 to 39 days before harvest) reduced the yield by 11-14%. Undesiccated control in both years achieved the highest yields, increase yield by 5-12%. Desiccation in the optimum date (17 days before harvest) increased the yield of attempts by 5-6%.
The term desiccation of winter rapeseed, also significantly affects the vitality of seeds, where very reduces energy germinating seedlings in the first days. The term desiccation, however, does not affect overall seed germination. Too early desiccation (46 to 39 days before harvest) EK2 decreased by 12-40%, EK3 decreased by 4-24%, 3-4% EK4 and extended MGT of 7-15%. In the first experimental year was the most vital option undesiccated control (EK2 = 50.4% = 91.3% EK3, EK4 = 97.9%). In the second experimental year was the most vital seeds of the optimal term desiccation, (Sixth term, 17 days before harvest) = 68.9% EK2, EK3 = 98.2%, EK4 = 100%. Seed samples of 2014/15 had a higher overall vitality of seeds, than samples from 2013/14, the overall vitality is probably worse given year old and transsilaged seed.
The results of the two-year experiment that term desiccation affects the quality of seeds, the yield of seeds and vitality of seeds. Pre-analysis was determined optimum solids content in siliques desiccated samples to values of 40-50%. Like most technology seems to desiccation in the optimum date (17 days before harvest) and agro technology without desiccated vegetation. As the least appropriate technology seems very early desiccation (46 to 30 days before harvest).
The first scientific hypothesis: Premature desiccation reduces seed yield, oil content and HTS. Yes, the hypothesis was confirmed.
The second scientific hypothesis: Desiccation made in the correct term do not affect the quality of the seeds (oiliness and HTS). Yes, the hypothesis was confirmed.
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The Evaluation of Reproduction in Bactrian Camels (Camelus bactrianus) and the Possibilities of Using Non-invasive Methods for Detection of Heat and Pregnancy
Fedorova, Tamara ; Lukešová, Daniela (advisor) ; Jiří, Jiří (referee)
Camels are important husbandry animals which are also often bred in zoological gardens. Unfortunately, camels in European zoos are not usually trained and pregnancy diagnosis in a half-tamed camel is very difficult. Moreover, information of the maternal behaviour of camels is limited. This thesis reviewed current knowledge on camel husbandry, reproduction and behaviour and aimed to 1) examine non-invasive methods of heat and pregnancy diagnosis from urine and saliva in camels kept in zoological gardens; 2) explore their maternal and suckling behaviour; 3) describe experiences with artificial rearing of camel calves. The research into non-invasive pregnancy diagnosis was carried out from 2010 to 2012. Urine from 14 camel females kept in four European zoological gardens was collected and tested using two chemical tests -- the Cuboni reaction and barium chloride test. The Cuboni reaction was significantly (p < 0.01) affected by the pregnancy status of female camels, and its accuracy increased significantly (p < 0.05) in the period leading up to parturition. The barium chloride test did not provide reliable results. Next, the saliva of five adult female camels was sampled for more than one year and concentrations of progesterone (P4) and oestradiol (E2) were measured. The concentrations of P4 (n = 312) and E2 (n = 310) were both significantly (p < 0.0001) affected by the pregnancy status of the animals. Maternal and suckling behaviour was observed from 2003 to 2009 in six zoological gardens, and the presented study includes partial data from this period. Allosuckling (i.e. when a female nurses a non-filial offspring) was described for the first time in camels and it represented 8.58% of all suckling bouts. The non-filial calves suckled more often in the lateral position and preferably joined the filial calf when suckling, so the results support the 'milk theft' hypothesis (stealing of milk) as a main cause of this behaviour. Finally, calf rearing in the Prague zoological garden was summarised and two camel calves were successfully artificially reared. This PhD thesis concluded that 1) the Cuboni reaction with urine and salivary P4 and E2 measurements are suitable methods for pregnancy diagnosis in half-tamed female camels; 2) allosuckling is relatively common in captive Bactrian camels; 3) the artificial rearing of camel calves with a calf milk replacer can be successful.
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Ecological and evolution strategies of necrophagous beetles (Coleoptera)
Jakubec, Pavel ; Růžička, Jan (advisor) ; Petr, Petr (referee)
Necrophagous beetles (Coleoptera) are very interesting and diverse ecological group of species with an immense impact on a natural nutrient cycle. Their main food source and breeding ground are carrions of vertebrates, human remains included. This relationship is often used in various ways by forensic entomology, but its potential was not jet fully reached, because our knowledge of biology and ecology of these beetles is very much incomplete. In this thesis I would like to explore geographic distribution, ecological requirements and developmental biology of several Central European necrophagous beetles as an outcome of their ecological and evolution strategies. For that I raised three broad research questions. Which factors are determining the local abundance of carrion beetles (Coleoptera: Silphidae)? How the current geographical distribution of open-landscape carrion beetles looks like in the Czech Republic? How the temperature affects the development of Sciodrepoides watsoni (Spence, 1813)? We found out that soil type can have significant effect on abundance of carrion beetles. They showed preference for chernozem -- Nicrophorus antennatus (Reitter), N. germanicus (Linnaeus), N. interruptus (Stephens), N. sepultor (Charpentier), Silpha obscura obscura (Herbst), T. sinuatus (Fabricius) or for fluvisol as did N. humator (Gleditsch). These findings support our hypothesis that soil type could be an important factor determining the occurrence of necrophagous European carrion beetles. To collect novel data of the current geographical distribution of carrion beetles we used 420 baited pitfall traps at 84 localities, and we collected 71 234 specimens of 15 silphid species. Among them, three endangered carrion beetle species listed on the Czech Red List of Invertebrates, were found. Two are vulnerable thermophilic species of open landscapes, Nicrophorus antennatus (Reitter, 1884) (collected around Louny and Židlochovice) and Nicrophorus germanicus (Linnaeus, 1758) (Louny, Zábřeh and Židlochovice). The third is the near threatened species, Nicrophorus sepultor Charpentier, 1825 (collected around Louny, Kutná Hora, Zábřeh and Židlochovice), which also prefers open landscapes. We studied development of common Holarctic beetle Sciodrepoides watsoni under five constant temperature regimes in laboratory (15, 18, 21, 25 and 28°C). Parameters of thermal summation models and their standard errors were calculated for each developmental stage (egg, three larval instars and pupae). We also find a new character for larval instar determination (head width) and proposed novel approach for future studies of size-based characters in instar determination.
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Factors influencing seed germination, emergence and mortality in soil seed bank of Bromus sterilis L.
Žďárková, Veronika ; Soukup, Josef (advisor)
Bromus sterilis L. (barren brome) spreads rapidly in many European regions. In the Czech Republic, its importance has increased dramatically over the past 10 years. Barren brome is reported as a problem weed in winter crops such as winter wheat, winter barley and oil seed rape, in vineyards and in other cultivated places. Barren brome has been becoming troublesome weed of winter cereals mainly in reduced soil tillage systems. The factors, that are important for its spreading and adaptability under different environmental conditions, are dormancy and germination. Optimal timing for seed germination varies with respect to natural conditions and it is determining for plant development. These traits are adapted to different conditions and habitats; therefore the dormancy and germination response patterns to those conditions vary significantly. Recently there are not many information available on germination behaviour, therefore this study was focused on seeds of barren brome and its characteristics, which were collected in different regions of the Czech Republic. Seeds were investigated under different temperatures, light regimes and water stress in a wide range of conditions. The following characteristics of seeds were studied the dormancy, the dynamics of germination, the temperature optimum, the age of seeds and dynamics of emergence from different depths and persistence in the soil profile under field conditions. Dormancy and germination are influenced by external conditions. The dormancy of seeds barren brome is very short or missing. The germination is influenced by light; the seeds germinated better under darkness than under light regime. The primary dormancy of B. sterilis was short and the seeds needed only three weeks for after-ripening. The seeds of B. sterilis showed broad ecological valence to hydrothermal factors germinating in the wide range of 3 to 35 °C. The temperature optimum is 5-23°C. The germination was only slightly influenced in an environment with low water potential. Germination was limited under water stress only at lower temperatures under 10°C. The response to light at various temperatures showed that seeds germinated better in darkness in all temperatures regimes, than in alternating light regime, especially at lower temperatures. The emergence declined significantly with burial depth (under 40 mm). The seeds were not able to survive in the soil seed bank for a longer time and fall seeds lost viability after 1 year burial in soil profile. These results may be of value for development of predictive models and understanding period when weed control may be most feasible.
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Possibility of increasing the quality of soybean seed
Procházka, Pavel ; Capouchová, Ivana (advisor) ; Milan, Milan (referee)
In three-years experiments soya seeds were treated with biologically active compounds: Lignohumate B (mixture of humic and fulvic acids), Lexin (mixture of humic and fulvic acids enriched by auxins), brassinosteroid (syntetic analogue of natural 24 epibrassinolide) and so-called complete seed treatment (mixture of saturated solution of sucrose, Lexin, fungicide Maxim XL 035FS and adjuvant on the base of pinolene Agrovital). During vegetation following characteristics were observed: field germination, density of vegetation after germination and before harvest, height of plants, height of apical part of the lowest pod from the ground and yield. The results imply that the most effective way of seed treatment is the complete seed treatment, which ensured the yield of 3.29 t of seeds per hectare (average for three years), the second best was the treatment with the compound Lexin, thanks to which the achieved yield was 3.18 t of seeds per hectare. Laboratory experiments showed that application of biologically active compounds Lignohumate B, Lexin and brassinosteroids has possitive effect on not only laboratory germination of seeds, but also on seed vitality (detected laboratory germination after accelerated aging test). All used biologically active compounds increased laboratory germination rate, rate of plants with opened cotyledons, rate of plants with opened true leaves and amount of above-ground and underground biomass in the initial phases of growth. In the same time, laboratory experiments proved, that even if chosen parts of evaluated varieties had very similar laboratory germination, among others declared by seed producer, vitality test (accelerated aging test) showed considerable differences in vitality (thus even in quality) of chosen parts of seeds. Three-years experiments showed, that desiccation of soya vegetation in BBCH phase minimally 89 (desiccated was mainly eventual weed infestation) does not have negative effect on quality of produced seeds. Desiccation was done in six different variations (used preparatives: Aurora 40 WG, Roundup Rapid, Reglone, Basta 15, Desicate, DAM 390). Results proved, that if we use the same preparatives in BBCH phase 79-85 (desiccated was mainly vegetation, which was photosyntheticly active), than preparatives (mainly Roundup Rapid, Basta 15 and partially Reglone) have negative influence on quality of produced seeds, mainly on their vitality (laboratory germination after accelerated aging test).
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Impact of rainwater management on mitigation of hydrological extremes
Pešková, Jitka ; Štibinger, Jakub (advisor) ; Miroslav, Miroslav (referee)
This dissertation deals with the impact of rainwater harvesting on mitigation of hydrological extremes. Due to the fact that it is a very broad topic that cannot be completely elaborated, the work targets selected aspects of this issue. The work is divided into two parts. The first part is divided into the following five chapters: The water in the landscape, Water regime, Hydrological extremes, Water regime adjustment and Legislation. These chapters summarize available worldwide knowledge about rainwater harvesting both in the landscape and in urbanized areas with a focus on mitigation of extreme hydrological phenomena (floods - drought), including legislative security of water management on national and EU level.
The second part of the thesis is compiled in the form of an annotated set of four scientific studies, whose results were presented in articles published in scientific journals. The first study targets the efficiency of torrent control (hydro-technical point of view and migration of water fauna). The second study focuses on the territory of the Morava River floodplains, where negative impacts of floods and droughts appear repetitively. The study solves the hydrological balance of the area using the WBCM-7 model. The third study focuses on computation method of the retention drainage capacity in the agricultural area drained by subsurface pipe drainage system. The last paper describes a new application of the Fourier series for detailed simulation of the runoff on a catchment in the dry periods. Within the thesis, these studies have been interconnected by explanatory comments.
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Influence of targeted grassing in part of the waterdhed on nitrate concentrations trend in drainage and subsurface waters
Zajíček, Antonín ; Štibinger, Jakub (advisor) ; Vymazal, Jan (referee)
Water quality in small agricultural slopy catchments in Bohemian - Moravian highlands is often threatened by high nitrate concentration and nitrogen loads from diffusive agricultural sources. The main goal of this thesis was to get a practical evidence for findings obtained in the Czech Republic and abroad by statistical approaches concerning the profoundly mitigative effects that grassing certain catchment areas has on the nitrate burden in drainage and surface waters. The research was conducted mainly in the Dehtáře experimental catchment, where part of the recharge area with an area of 4.6 ha, has been grassed since the hydrological year 2007. Together with long-term monitoring of nitrate leaching in subcatchments with different land use in recharge and discharge are, drainage runoff generation and composition were surveyed in order to find knowledge about the recharge area placement and its importance for drainage water quality. The possibility of using the continuous measurement of water temperature for drainage runoff separation was also tested. The results presented show that nitrate concentration values in drainage water were influenced the most by the land use of the recharge zones within the drainage subcatchment. While permanent grassland placed directly in the drained area (corresponding to the catchment discharge zone) did not show any influence, the grassing focused on the catchment recharge area demonstrated a significant decrease in both, NO3 concentrations and N loads. Approximately one year after grassing, the long-term course of nitrates concentrations changed direction and became decreasing in sites with completely or partly grassed recharge zone, while in sites without land use change), the nitrate concentrations trend remains increasing or the stagnation was found. Decreases by 32% was detected in systematic drainage subsystem, which recharge area was completely grassed. The nitrate-nitrogen leaching decreased after grassing in recharge area of the drainage system by 23%. These findings can be generalised for slopy agricultural catchments with common land use in soil environments formed on crystalline rocks. The acquired findings are of high importance for improving the water quality of small streams as well as groundwater in agriculturally exploited areas, for planning protective zones within large catchments of potable water reservoirs, and also for protecting small local surface or groundwater sources of potable water.
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