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Teaching Biotechnology at Secondary School
Ouřadová, Věra ; Čížková, Věra (advisor) ; Drda Morávková, Alena (referee)
Biotechnology accompanies human society from its distant past and certainly will have a major impact on its future. For this reason, young people should become familiar with biotechnology in matters of its nature, potential and risks, not only through the media, but also and particulary in context of school education. This work is dedicated to teaching of biotechnology at the secondary level, and its first objective is to characterise its current state. For this purpose an analysis of curriculum and textbooks of secondary level was performed and indicative survey of knowledge of biotechnology and attitudes towards it among students aged 14-19 years was conducted. The second goal is to propose a possible way of including biotechnology topics in education, firstly as a part of school curriculum, and furthermore through educational materials oriented on the practical work of students connected with active knowledge acquisition and shaping their attitude towards it.
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Geneticky modifikované potraviny
Tuzová, Kristýna
Diploma thesis Genetically modified foods discusses about genetically modified foods in the Czech Republic, European Union (EU) and worldwide. The aim of the thesis was to investigate the awareness of Czech and Austrian students and citizens about geneti-cally modified foods and also to highlight the pros and cons of genetically modified foods. The method of literary research and comparative method was used to evaluate the current state of genetically modified food. In the experimental part of the thesis a brainstorming method and SWOT analysis were used to determine the main advantages and disadvantages associated with genetically modified foods. Information on the atti-tudes of interest groups was collected through a questionnaire survey. The results show that the awareness and attitude of the general public and students varies within the Czech Republic and Austria.
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Geneticky modifikovaná krmiva
Štefan, Petr
Bachelor thesis deals with genetically modified feeds, which are currently the future in animal nutrition. It deals with the history and development of biotechnology. It also deals with legislative issues in the field of GMO in the Czech Republic, the European Union and in the world. It discusses the importance of GM crops/feeds in animal nutrition. It maps the cultivated crop statistics and the countries most concerned with growing GM crops.
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Biotechnické metody reprodukce skotu
Trávníčková, Barbora
This bachelor thesis is focused on description of basic biotechnological methods used in cattle breeding, their success and comparison in significant world countries. Attention is focused on basic biotechnological methods, such as insemination, embryotransfer (IVP), synchronization, sexing of insemination doses and embryos. In the most commonly used methods in reproduction, insemination describes the methods of detection of rut. Insemination reduces the spread of infection, improves fertility and improves genetic gain. At the end of the thesis, the percentage of success of basic reproductive biotechnology methods and comparison of methods from the economic point of view.
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Stress responses in Rhodococcus strains
Křenková, Lucie ; Pátek, Miroslav (advisor) ; Cajthaml, Tomáš (referee)
Throughout the evolution, bacteria developed ingenious mechanisms which help them survive and grow in harsh conditions, where extreme temperatures, pH, changing water and osmotic activity occurs. Rhodococcus sp. cope with these conditions so efficiently they are able to grow in the presence of toxic compounds. Large genome of Rhodococcus sp. consists of a great amount of genes involved in a broad spectre of catabolic pathways. To use these skills in biotechnology, it is necessary to know stress response mechanisms well. In response to the changing environment Rhodococcus sp. use transcriptional regulators, two-component system and RNA polymerase sigma factors to tune the expression profiles of the genes. The upregulation of chaperons, chaperonins and proteases is the main pattern followed in most of the stress responses. Vast majority of stress responses is complex and groups of genes to react on them interfere too. Thus, it is very difficult to interpret the data of these studies. However, the great importance of understanding these mechanisms is unquestionable. Key words: stress response, bacteria, Rhodococcus, biotechnology, degradation, toxic compounds, sigma factor
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Preparation of strategy for studying interaction of human constitutive androstane receptor with steroid receptor coactivator-1
Šachová, Ivana ; Martin, Jan (advisor) ; Mladěnka, Přemysl (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Ivana Šachová Supervisors: PharmDr. Jan Martin, PhD., Dipl. Ing. Mgr. Ferdinand Molnár, PhD. Title of diploma thesis: Preparation of strategy for studying interaction of human constitutive androstane receptor with steroid receptor coactivator-1 The aim of this study was to express wild type and mutated synthetic human SRC1 central domains in E. coli BL21 DE3 cells. Expressed recombinant proteins were purified afterwards and used for investigation of SRC1-CAR protein interaction using pull down experiments. The constitutive androstane receptor (CAR), a nuclear receptor (NR), is a transcriptional regulator, which influences the expression of various proteins, such as enzymes of biotransformation and transporters important for metabolism of both endogenous and exogenous compounds. CAR can be fully active only in presence of coactivators, such as steroid receptor coactivator-1 (SRC1). The most important fragment of SRC1 for binding to NRs is the central domain, made of three α-helical Leu-X-X-Leu-Leu (LXXLL) L1, L2, L3 motifs, in where L is a leucine and X represents any amino acid. hSRC1, as well as hCAR, can be successfully produced by expression of cDNA of interest in E. coli strains. In case of SRC1,...
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Overview of phylogeny, systematics and diversity of genus Ulva (Ulvophyceae) and biotechnological use of these algae
Kantnerová, Veronika ; Neustupa, Jiří (advisor) ; Němcová, Yvonne (referee)
The genus Ulva represents a remarkable group of organisms, with number of species. It belongs to the class Ulvophyceae, one of the major groups of chlorophytic green algae and it is characterized by a typical dual morphology and isomorphic haplo-diplontic life cycle. This work is a summary of existing knowledge related to this algal genus with regard to the phylogeny, diversity and ecology, which is relatively interesting due to the ability of these algae to adapt to a wide range of abiotic conditions. It also focuses on the development of thallus morphology of these algae, especially with relation to an interesting and very important role of symbiotic bacteria in this process and, last but not least, on the biotechnological utilization that has great potential for the future, especially in terms of the utilization of Ulva as an alternative renewable energy source, a food supplement with high content of important proteins, minerals and vitamins, or as a source of polysaccharides, the so-called "ulvans", which are now investigated for their biological activity and application in human medicine. Key words: Ulva, Ulvophyceae, phylogeny, morphogenesis, biotechnology, ekology, diversity
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Geneticky modifikované organizmy v potravinách
Košíková, Romana
My bachelor thesis genetically modified organisms in food deals zoom GMOs in terms of crops , animals and microorganisms and their use in the world. The aim was to bring positive and also negative prospects associated with the use of GMOs . The paper referred to the legislation in force and labeling GMO foods.
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