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Impact assessment of Act No. 254/2019 Coll., On Experts, Expert Offices and Expert Institutes
Somerlíková, Martina ; Výskala, Miloslav (referee) ; Aigel, Petr (advisor)
The final thesis deals with the issuance of Act No. 254/2020 Coll., On experts, expert offices and expert institutes. The aim is to analyze, describe and evaluate the impact of the law change on expert activities. The analysis took the form of a questionnaire survey addressing 1,002 experts. The evaluation of the results is processed using graphs, tables and free citations of experts. The thesis deals with the characteristics of individual provisions of the law, their negative effects and benefits. The output of the work is an analysis of the current situation. In the end, recommendations and proposals for measures are formulated.
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Assessment of fatty acids in processed cheese
Pruknerová, Kateřina ; Zemanová, Jana (referee) ; Vítová, Eva (advisor)
This thesis is focused on determination of fatty acids in processed and/or processed cheese analogues using gas chromatography with FID detection. Characterization of fatty acids, various methods for lipid extraction and the possibilities of determination of fatty acids are described in the theoretical part. Simple and rapid method for the determination of fatty acids in natural and/or processed cheese was optimized and validated in the experimental part. The modified Folch method (by mixture of chloroform - methanol) was selected for lipid extraction from the samples. The standard method (ISO 5509), using a methanol solution of potassium hydroxide, was selected for lipid esterification. Type of the stationary phase and parameters of the column, flow of carrier gas, temperature of the injector, injection technique, temperature of the detector and temperature program were optimized. Calibration curves were constructed from five calibration points (in the concentration range of 0.01 - 60 g.ml-1), all correlation coefficients R2 were higher than 0,99. The limits of detection and quantification of individual fatty acids ranged from 0.002 - 6 g.ml-1. The repeatability of the method was good, all the relative standard deviations of the peaks were below 9 %. The optimized and validated method was applied to selected samples of processed cheese analogues. In total five types of analogues containing different kinds of fat (butter, apricot, linseed, black currant and grape seed oils) were analyzed. In samples 34 fatty acids were identified based on a comparison of retention times of standards.
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Study of aggregation process and physical stability of polymeric micelle by fluorescence probe method
Chovancová, Romana ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
Amphiphilic block copolymer poly(ethylene glycol)-b-poly(–caprolactone) (PEG–PCL) was synthesized from poly(ethylene glycol) (PEG) and poly(–caprolactone) (PCL) by using ring-opening polymerization. The structure and composition of this copolymer was determined by IR spectroscopy. Polymeric micelles were prepared by membrane dialysis method and direct dissolution of copolymer in water. The process of aggregation and physical stability in water solution was studied by fluorescence spectroscopy using pyrene and perylene as fluorescent probes.The results of steady-state and time resolved fluorescence measurements indicate that system of PEG–PCL forms both unimolecular and multimolecular micelles, which depends on copolymer concentration. The critical micelle concentration (CMC) was around 0,002 g/L. Measurement of micelles size by dynamic light scattering method suggested that systems with higher concentration form bigger aggregates. In addition, copolymer behavior was explored under physiological conditions. The results show that CMC of copolymer increased in 0,15 mol/L sal solution at temperature 37°C to 0,02 g/L when compared to copolymer in water.
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Tolerance of DNA damage by novel biologically active platinum complexes
Vystrčilová, Jana ; Vrána, Oldřich (referee) ; Nováková,, Olga (advisor)
The anti-tumor activity of platinum based drugs is mediated by their ability to attack DNA. Platinum complexes can alter the structure of DNA by modifying the bases, mainly guanines. The biological consequnces of such interactions are compromising replication and transcription. RNA polymerase complex can stall at a damaged site in DNA and mark the lesion for repair by proteins that are utilized to execute nucleotide excision repair, a pathway commonly associated with the removal of bulky DNA damage from the genome. This RNA polymerase-induced repair pathway is called transcription-coupled nucleotide excision repair. Main goal of this thesis was to study RNA polymerases tolerance of DNA damage by novel, biologically active platinum (II) complexes involving derivatives of aromatic cytokinines as the ligands; cis-[Pt(2-chloro-6-(4-methoxybenzylamino)-9-isopropylpurin)2Cl2](PR-001), cis-[Pt(2-chloro-6-(benzylamino)-9-isopropylpurin)2Cl2](PR-002 )and cis-[Pt(2-(3-hydroxypropylamino)-6-(benzylamino)-9-isopropylpurin)2Cl2](PR-005). DNA templates (constructs) that contain a single, site-specific DNA lesion and support transcription by human RNA polymerase II and bacteriophage T7 RNA polymerase were prepared. The method is making use of polymerase chain reaction (PCR) and biotin-streptavidin interactions and paramagnetic particles to purify the final product. Synthetic oligomers duplexes (75-mer, 56-mer and 15-mer) are ligated to 5´-biotin pCI-neo-G-lessT7 PCR fragment, the 15-mer is either unmodified or modified with a site-specific lesion of PR-005 and cisplatin. We also studied the inhibition of RNA polymerases activity on globally modified plasmid pCI-neo and pUC 19 by novel platinum complexes and cisplatin. We found that bifunctional adducts of complex PR-005 contrary to adducts of PR-001 and PR-002 effectively decrease amount of full lenght transcripts produced by both, human and bacterial RNA polymerases. This result can be explained by a sterical block, induced to DNA by intrastrand cross-link of PR-005 with bulky aromatic ligands.
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Precise Real-Time GNSS Positioning Using Different Correction Representation
Nosek, Jakub ; Václavovic, Pavel
The paper deals with the comparison of the Real Time Kinematic (RTK) method and the Precise Point Positioning (PPP) method with fast ambiguity resolution (PPP-RTK). Both methods use different correction models that affect their results. Data from 6 permanent GNSS stations from 28th August 2022 to 30th August 2022 were processed. The results show that the RTK method provides increasingly accurate results (standard deviations of the NEU coordinate components sN = 0.003 m, sE = 0.002 m, sU = 0.006 m). The PPP-RTK method provides slightly worse results (sN = 0.011 m, sE = 0.009 m, sU = 0.028 m). On the other hand, it is more independent of current problems in the network of reference stations.
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Nutrition and Other Characteristics of Infants
NOVOTNÁ, Radka
The main goal of this bachelor thesis was a processing of research based on interesting literary and electronic sources which primarily deal with children's nutrition. Closely related topics include, for instance, disease rates or vaccination of children. Another aim of this thesis was to collect data through questionnaires followed by their comparison. This bachelor thesis consists of two parts - theoretical and empirical. The first part presents information acquired and processed from domestic and foreign literature. This thesis concerns the importance of breastfeeding, supplementary feeding or non-dairy formula feeding. It also focuses on the types of breast milk or breastfeeding with its positive and negative effects on an infant. In addition to the beforementioned topics, this thesis gives a detailed description of mandatory and most common optional vaccines, including a brief mention of disease rates of children. The empirical part of the thesis focuses on the evaluation of data collected by the questionnaire method. The beforehand formulated questionnaire is part of a larger project linked to the works of the following students: Markéta Mužíková, Tereza Šafránková and Kateřina Součková. The questionnaire for legal representatives of children was composed in an online form. Respondents' parents filled in the data predominantly from the Health and Vaccination Cards. Data from 110 respondents have been collected in total. Fifty-six respondents were girls and fifty-five boys. Afterwards, our selected data were compared with the CAV 2001 Nationwide Anthropological Survey. In the vaccination category, it was found that each individual had received all the mandatory vaccines given by law. Prevenar 13, among the non-mandatory vaccines, was the most frequently used vaccine in 39 cases out of 110 respondents. In the category of childhood morbidity rate, the following childhood diseases were examined - catarrh of the upper respiratory, allergy, eczema and acute otitis media. It was found that most of the non-breastfed children of our cohort were overall less ill than the breastfed children. This result is consistent with the research of the student Tereza Šafránková but is not in accordance with some professional publications, for example (Muntau, 2014) or (Nevoral et al., 2013). In the last part of the research, which concerned the body measurements of children up to one year of age, the results show that non-breastfed individuals are more likely to exceed breastfed children in average development. The basic body measurements of children from birth to 1 year were compared with the results of the CAV 2001 Nationwide Anthropological Survey (CAV, 2001). Statistically significant values were found for body weight of breastfed one-year-old boys (p=0.000), body length of six-month-old (p= 0.007) and one-year-old breastfed boys (p=0.001), chest girth at birth for girls (p= 0.001) and boys (p= 0.004), head girth at birth (p= 0.000) and at six months for breastfed boys (p= 0.003), and BMI at birth for non-breastfed girls (p= 0.002).
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PREPARATION AND CHARACTERIZATION OF NATURAL EXTRACTS WITH ANTIMICROBIAL EFFECT
Vysoká, Marie ; Obruča, Stanislav (referee) ; Kráčmar, Stanislav (referee) ; Márová, Ivana (advisor)
Celosvětová spotřeba antibiotik v posledních několika desetiletích významně vzrostla, což vedlo ke zvýšenému výskytu genů antibiotické rezistence (ARG), které představují rostoucí vážnou hrozbu pro lidské zdraví na celém světě. Používání přírodních nebo alternativních léčivých přípravků tudíž v posledních několika letech výrazně vzrostlo. Velká pozornost je nyní věnována přírodním extraktům, které by mohly být v jisté formě použity jako jedna z možností léčby bakteriálního onemocnění, a to s vysokou účinností proti patogenům a se zanedbatelnými vedlejšími účinky. Biomasa kvasinek, která obsahuje cenné mastné kyseliny, sacharidy, nukleové kyseliny, vitamíny a minerály, byla testována jako možná alternativa, a to samotná i v kombinaci s rostlinnými extrakty ve formě doplňku stravy. Tato disertační práce je zaměřena na studium antimikrobiální aktivity a nutriční přínosy vybraných rostlinných extraktů, jako je acai, hřebíček, kurkuma, zázvor, ženšen a oregano. Následně byly testovány biologické účinky kvasinkové biomasy rodu Metschnikowia pulcherrima CCY 029-002-145, Cystofilobasidium infirmominiatum CCY 17-18-4, Phaffia rhodozyma CCY 77-1, Rhodotorula kratochvilovae CCY 20-2-26. Tyto kvasinky byly vybrány zejména pro vysoký obsah aktivních látek v produkované biomase. Na závěr byl posouzen potenciální synergický efekt rostlinných extraktů ve spojení s kvasinkovou biomasou ke zvýšení celkového antimikrobiálního účinku, antioxidační aktivity a vlivu na růst buněčných linií Caco-2, B16F1 a HaCaT. Vzhledem k dosaženým výsledkům lze konstatovat, že zařazení imortalizované kvasinkové biomasy v kombinaci s přírodními extrakty do doplňků stravy ve vhodné formě může být prospěšné lidskému zdraví. Existuje přímý vztah mezi životaschopností buněk a apoptózou. Všechny mikrobiální extrakty vykazovaly určité pozitivní výsledky na apoptózu u rakovinných buněčných linií a současně nevykazovaly žádné cytotoxické účinky na nezhoubné buněčné linie při stejných koncentracích.
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