National Repository of Grey Literature 9 records found  Search took 0.00 seconds. 
Nanoparticle-Mediated Delivery System for Gene Therapy
Dvořáková, Nikola ; Ellederová, Zdeňka (advisor) ; Šálek, Petr (referee)
Gene editing with the CRISPR/Cas9 system is one of the options that sets a new trend in the development of gene therapy. The most commonly used delivery of DNA into the cells are via viruses. Nevertheless, they are often unable to take CRISPR/Cas9 system, which can be bigger than several kb. Nanoparticles (NPs), as non-viral transporters, seem to be a good alternative delivery system. For this work magnetic Fe3O4 NPs (MNPs) were selected, because of their excellent properties such as multifunctionality, biocompatibility, easy degradation and simple synthesis. The aim of this work was to synthesise MNPs and a complex of MNPs coated with PEI/CRISPR-Cas9 plasmid and to characterize them by physicochemical methods. The created complex MNPs/PEI/CRISPR-Cas9 was defined by exact parameters that are suitable for possible cell uptake. The hypothesis of stabilization of the MNPs/CRISPR-Cas9 plasmid complex by polyethylenimine (PEI), which can also protect plasmid DNA against restriction endonucleases, was verified. Next a stable modified cell line HEK293-TLR3, designed to evaluate the efficacy of double strand break (DSB) repair by nonhomologous end joining (NHEJ) or homologous recombination (HR) was, transfected with the synthesised MNPs/PEI/CRISPR-Cas9 complex. The results indicate a 25% transfection...
Benefits of Neoadjuvant Therapy in Colorectal Cancer Treatment
DVOŘÁKOVÁ, Nikola
This Bachelor's thesis focuses on benefits of neoadjuvant therapy for colorectal cancer. The thesis is divided into a theoretical and a practical part. The theoretical part contains data I have compiled from available literature, scholarly articles and professional websites. I concentrated most closely on colon and rectal anatomy, screening, diagnosis and treatment of colorectal cancer. The aim of the Bachelor's thesis is to compare the extent of colorectal cancer before and after neoadjuvant therapy. Furthermore, the second aim is to determine the number of radically operated patients after preoperative treatment. The practical part consists of data from the archive of medical records at the Department of Oncology in the České Budějovice Hospital, a. s. The observed factors were age and gender of the patient, diagnosis, localization and TNM classification, type of neoadjuvant therapy, initiation and termination of therapy, type of operation and its initiation. The last factor was the extent of disease after surgery. In total, I evaluated 50 patients who met the criteria. I processed the data into comprehensive graphs and spreadsheets. The collected data show an improvement in the extent of disease in 84 % of patients. 5 % of patients had the same TNM classification and the disease worsened in 11 % of patients. Complete or nearly complete remission occurred in 6 patients (14 %). 88 % of patients from the whole research group were radically operated. 20 patients (40 %) underwent rectal resection and 24 patients (48 %) underwent rectal amputation. 6 remaining patients did not have any surgery. Two patients had an inoperable tumour, others were not operated due to their high age, weight or because it was their wish. The results from the practical part can provide feedback to the Department of Oncology in the České Budějovice Hospital. Next, it can also serve as a study material for students and the general public.
Potential of Prague theatres in domestic and incoming tourism
Dvořáková, Nikola ; Jarolímková, Liběna (advisor) ; Netková, Jarmila (referee)
The main goal of this thesis is to evaluate the fulfillment of the potential of Prague theatre institutions in the domestic and incoming tourism of the Czech republic, to find the most significant Prague theatre institutions and compare the way they present their offer with institutions in competitive destinations. The first two chapters introduce the cultural tourism in general, in the Czech republic and in Prague. The following chapter describes the offer of Prague theatres and the fulfillment of its potential, where the evaluation is based on the results of a research, carried out by the author herself. The last chapter presents a comparison of presentation on websites of chosen theatres in Berlin, Dresden and Vienna with Prague.
Generation of large animal models using genome editing
Dvořáková, Nikola ; Ellederová, Zdeňka (advisor) ; Kašpárek, Petr (referee)
The principle of gene engineering is the intervention to the DNA of the studied organism. After the discovery of the programmed endonucleases, there has been a great expansion of this technique and it also accelerated the possibilities to create large animal models. Until recently, large animal models were very difficult to be generated. These endonucleases include zinc finger nuclease (ZFN), transcription activator like effector nuclease (TALEN) and CRISPR/Cas9. All endonucleases produce locally specific splicing in the targeted segment of the genome. This splicing is most easily corrected by the non-homologous ends joining (NHEJ), so then it is possible to create a so -called knock-out (KO) model. The second type of repair is homologous recombination (HR) using a DNA template with homologous arms. This makes it possible to create a knock-in (KI) model that cannot be created without specific endonucleases in large animal models due to the low natural HR. This work summarizes the history, technique and the use of programmed endonucleases for the creation of large animal models. These models have a great use in biomedicine, mostly in preclinical research, they are also significant in agriculture and even in the environment protection. Key words: large animal model, transgenesis, genome editing,...
Contemporary art in school - post internet age
Dvořáková, Nikola Tora ; Kitzbergerová, Leonora (advisor) ; Daniel, Ladislav (referee)
TITLE: Contemporary art at school - post internet age AUTHOR: Nikola Tora Dvořáková DEPARTMENT: Department of art education SUPERVISOR: Ph.Dr. Leonora Kitzbergerová, PhD. ABSTRACT: The thesis - Contemporary art at school - post internet age is about current arts occasions, concretely Post internet art. I try to state and define this kind of art and place post internet art to context with art education afterwards. Specific art pieces and their similarities in related zones of art are reflected in didactic transformations. The research is focused on difficulties, which can come into the art education lessons. It is realized with different methods, on different kind of school. In concluision my original art piece is bringing more comprehensive knowledge about post internet art. KEYWORDS: Contemporary art, post internet art, interpreter vs. consumer, new media
"Electric houses" - architecture and equipment of progressive tenement houses in late 1930's in Prague
Dvořáková, Nikola Tora ; Biegel, Richard (advisor) ; Macek, Petr (referee)
The bachelor thesis is focused on the phenomenon of electric houses in Prague namely rental houses from the second half of 1930s. Their basic element is the omnipresent electric enegry which fundamentally changed the living conditions in the apartment houses. Electric houses architecture is based on the international style principles which blend the luxourious living standards located in the suburbs with more economical city apartments. Electric houses represent spectacular form of housing beside the contemporary trend of minimal residential units. These houses became one of the major Prague street components despite the excessive luxury and supposed uneconomical electric energy. They replaced the period of family houses in the suburbs. The thesis deals with the principles and terms connected to these electric houses presented on several different architectural projects.
Revitalisation of the historical garden of the Castle Liblice
Dvořáková, Nikola ; Vaněk, Jan (advisor) ; Sylvie, Sylvie (referee)
This diploma thesis of project charakter, deals with revitalization castle garden of castle Liblice in central region of Czech Republic. The main goal of this thesis is based on the curent and historical state of the castle garden, to design a project with new plantings in the parter so it imitates the original baroque style in whitch it was created efore. After that propose suggestions of procedures suitable for the revitalization of the garden and it is greenery. Theoretical part of the thesis called Literary review is focused not only on history of the village where the castle is situated but also on the history of the castle itself, baroque gardens in general and least but not last the architect of the castle and it is garden. There are also described general characteristic of the trees and shrubs, which are in the garden as well as descriptions of work during revitalization such as tree cutting techniques or lawn care. In the analytical part is briefly described charakter of the area from its location to its climatic data. There is also described curent state of the castle park focusing mainly on the architectural errors and interventions, which are going to be necesary to execute during the revitalisation. List of greenery contains all trees and shrubs in the garden since 2005 and color features the ones removed during past years. After the consultation with the director of the castle there are mentioned activities and actions for which is the castle and garden used nowadays most of which also contributes along with the curent survy to the suggestions and interventions suitable for this revitalization. Project part deals with the design of the parter behind the castle. This design is created so it imitates the former baroque parter as much as possible, but also respects condotions of its curent use. All expenses in this design will be calculated in the form of ekonomic balance. Lastly in this project, there are proposed another important interventions for revitalisation of the castle garden, They are in the form of current state photos with drawn in suggested changes. This thesis will be beneficiary for the Czech Academy of Sciences, which owns this castle and it is garden .
Christianization of early medieval society in the view of the movable archeological sources
Dvořáková, Nikola ; Štefan, Ivo (advisor) ; Sláma, Jiří (referee)
The presented text deals with a process of Christianization with taking account of informative value of archaeology. Movable archaeological artifacts generally connected with Christianity are paid particular attention. These objects were divided into two groups: Group I - artifacts primarily linked to Christianity, Group II - artifacts with practical or decorative function carrying Christian theme. One category of objects was chosen from both the groups (Group I - hanging crosses, Group II - Strap end) and analysed. At the end a contribution towards knowledge of the process of Christianization is evaluated.
Financial analysis of the company
Dvořáková, Nikola ; Pevná, Jana (advisor) ; Svoboda, Hubert (referee)
The subject of this thesis is the evaluation of the financial situation of a company with limited liability by financial analysis methods in the years 2008-2012 and then the comparison the indicators of financial analysis of the company with sectoral data. This work is drawn up from the perspective of the external user via publicly available financial statements. In the methodological part of the thesis there are on the basis of literature processed knowledge about financial analysis. In the practical part are the indicators of financial analysis applied to the financial statements of the company and further are compared the individual indicators of the company with the sectoral indicators of financial analysis. The output of the work is to valorize the results of the calculations of the individual indicators of financial analysis and subsequently possible recommendations for improving the financial situation of the company.

See also: similar author names
8 DVOŘÁKOVÁ, Nikola
2 Dvořáková, Nela
1 Dvořáková, Nela Františka
2 Dvořáková, Nicolette
2 Dvořáková, Nikola Tora
8 Dvořáková, Nina
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