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Analysis of active phenolics in some cereal products
Vondráčková, Hedvika ; Vávrová, Milada (referee) ; Márová, Ivana (advisor)
Presented diploma thesis was focused on study of phenolic substances in various cereal products. Flours, brans and germs from various cereals, flakes and extruded cereals were chosen as samples. Phenolic substances were analyzed by spectrophotometry, RP-HPLC/UV-VIS and RP-HPLC/PDA/ESI-MS. Studies of saccharide content which were analyzed by spectrophotometry and HPLC/RI was incorporated to this thesis. Differences in the content of phenolic substances released from glycosides after acid hydrolysis of samples and differences in saccharide content determined after acid and enzymatic hydrolysis of samples were investigated. Simple sensory analysis of extruded cereal products of different flavour (cocoa, honey, chocolate) was carried out too. In analyzed samples the highest content of total phenolics and total flavonoids was measured in wheat germs. Especially in wheat germs and also in buckwheat and oat flakes, maize brans and cereals products Teddy (cereal balls with cocoa) and Ferda (cereal grains with honey) high levels of individual catechins, phenolic acids and flavonoids were determined too. The quantity of phenolic substances increased for several times in samples processed by acid hydrolysis in comparison to non-hydrolyzed samples. The high content of total saccharides, reduced saccharides and individual mono- and disaccharides was found mainly in extruded products. Concentration of monosaccharides increased in acid hydrolyzed samples in spite of disaccharides concentration that was higher only in some of hydrolyzed samples. In several cases final disaccharide concentrations were lower in hydrolyzed samples than in non-hydrolyzed ones.
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Acyclovir release from branched oligoesters
Vondráčková, Hana ; Šnejdrová, Eva (advisor) ; Dittrich, Milan (referee)
11 Summary The objective of this graduation thesis was a description of a liberation of aciclovir from plastificated oligoesters drug carriers. We used terpolymers of D,L-lactic acid, glycolic acid and dipentaerythritol or tripenaterythritol [LA/GA/D(T)]. Theoretic part is devote to processes during a liberation of a medicament from a polymer carrier also to polyesters of lactic acid, glycolic acid and their copolymers who have a remarkable impact in the field of controlled drug delivery and as well to pentaerythritol. At the experimental section we used disolution tests in order to obtain a concentration of released aciclovir by spectrophotometry. Process of liberation was diagrammatized like a dependence of cumulative precentage of released drug at a time. The results indicate that the carrier 3T is the best drug delivery vehicle for aciclovir. 3T is disposing the highest factor of branching and it has the biggest molar weight.
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Relationship between compensatory status and renal function in patients with diabetes mellitus type II
Vondráčková, Helena ; Szotáková, Barbora (advisor) ; Boušová, Iva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Bc. Helena Vondráčková Supervisor: prof. Ing. Barbora Szotáková, Ph.D. Specialist consultant: MUDr. Pavel Pick Title of diploma thesis: Relationship between compensatory status and renal function in patients with diabetes mellitus type II Diabetes mellitus is a chronic disease accompanied by a number of complications. Among the most common and at the same time the most serious are kidney diseases, which often lead to their failure. These patients then rely on lifelong dialysis treatment or a possible kidney transplant. By compensating for diabetes mellitus effectively, the development of complications can be reduced or at least delayed. Fasting and postprandial blood glucose level and glycated hemoglobin are used to assess the state of compensation. The renal function is assessed by the examination of albumin and proteins in urine, serum creatinine concentration and estimation of glomerular filtration. The aim of the thesis was to determine whether there is a relation between the level of compensation for diabetes mellitus and the renal function. The data from 70 patients diagnosed with diabetes mellitus type II regularly checked in a diabetological clinic were evaluated. Samples were...
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Relationship between compensatory status and renal function in patients with diabetes mellitus type II
Vondráčková, Helena ; Szotáková, Barbora (advisor) ; Boušová, Iva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Bc. Helena Vondráčková Supervisor: prof. Ing. Barbora Szotáková, Ph.D. Specialist consultant: MUDr. Pavel Pick Title of diploma thesis: Relationship between compensatory status and renal function in patients with diabetes mellitus type II Diabetes mellitus is a chronic disease accompanied by a number of complications. Among the most common and at the same time the most serious are kidney diseases, which often lead to their failure. These patients then rely on lifelong dialysis treatment or a possible kidney transplant. By compensating for diabetes mellitus effectively, the development of complications can be reduced or at least delayed. Fasting and postprandial blood glucose level and glycated hemoglobin are used to assess the state of compensation. The renal function is assessed by the examination of albumin and proteins in urine, serum creatinine concentration and estimation of glomerular filtration. The aim of the thesis was to determine whether there is a relation between the level of compensation for diabetes mellitus and the renal function. The data from 70 patients diagnosed with diabetes mellitus type II regularly checked in a diabetological clinic were evaluated. Samples were...
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Glycated hemoglobin: one of the indicators of compensation Diabetes mellitus
Vondráčková, Helena ; Raisová Stuchlíková, Lucie (advisor) ; Boušová, Iva (referee)
Diabetes mellitus belongs to the chronic civilization diseases with increasing inter-incidence. This Disease is connected with numerous complications that increase patients mortality and morbidity. The aim of the cure is to compensate and delay the development of especially late complications of diabetes. The bachelor thesis is focused on glycated hemoglobin, which is currently considered the primary method in evaluation of the diabetes mellitus compensation. The theoretical part is focused on basic characteristics and associated complications with the attention paid to the evaluation of the disease compensation. The practical part is focused on methods of determination of glycated hemoglobin, relations among the patients' levels of glycated hemoglobin, average blood glucose and BMI. Key words: Diabetes mellitus, glycated hemoglobin, diabetic retinopathy, diabetic nephropathy, diabetic neuropathy.
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Acyclovir release from branched oligoesters
Vondráčková, Hana ; Šnejdrová, Eva (advisor) ; Dittrich, Milan (referee)
11 Summary The objective of this graduation thesis was a description of a liberation of aciclovir from plastificated oligoesters drug carriers. We used terpolymers of D,L-lactic acid, glycolic acid and dipentaerythritol or tripenaterythritol [LA/GA/D(T)]. Theoretic part is devote to processes during a liberation of a medicament from a polymer carrier also to polyesters of lactic acid, glycolic acid and their copolymers who have a remarkable impact in the field of controlled drug delivery and as well to pentaerythritol. At the experimental section we used disolution tests in order to obtain a concentration of released aciclovir by spectrophotometry. Process of liberation was diagrammatized like a dependence of cumulative precentage of released drug at a time. The results indicate that the carrier 3T is the best drug delivery vehicle for aciclovir. 3T is disposing the highest factor of branching and it has the biggest molar weight.
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Analysis of active phenolics in some cereal products
Vondráčková, Hedvika ; Vávrová, Milada (referee) ; Márová, Ivana (advisor)
Presented diploma thesis was focused on study of phenolic substances in various cereal products. Flours, brans and germs from various cereals, flakes and extruded cereals were chosen as samples. Phenolic substances were analyzed by spectrophotometry, RP-HPLC/UV-VIS and RP-HPLC/PDA/ESI-MS. Studies of saccharide content which were analyzed by spectrophotometry and HPLC/RI was incorporated to this thesis. Differences in the content of phenolic substances released from glycosides after acid hydrolysis of samples and differences in saccharide content determined after acid and enzymatic hydrolysis of samples were investigated. Simple sensory analysis of extruded cereal products of different flavour (cocoa, honey, chocolate) was carried out too. In analyzed samples the highest content of total phenolics and total flavonoids was measured in wheat germs. Especially in wheat germs and also in buckwheat and oat flakes, maize brans and cereals products Teddy (cereal balls with cocoa) and Ferda (cereal grains with honey) high levels of individual catechins, phenolic acids and flavonoids were determined too. The quantity of phenolic substances increased for several times in samples processed by acid hydrolysis in comparison to non-hydrolyzed samples. The high content of total saccharides, reduced saccharides and individual mono- and disaccharides was found mainly in extruded products. Concentration of monosaccharides increased in acid hydrolyzed samples in spite of disaccharides concentration that was higher only in some of hydrolyzed samples. In several cases final disaccharide concentrations were lower in hydrolyzed samples than in non-hydrolyzed ones.
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Biologically significant substances in cereal products
Vondráčková, Hedvika ; Halienová, Andrea (referee) ; Márová, Ivana (advisor)
Presented work was focused on study of biologically significant substances in cereal products. In experimental part selected active substances in cereal bars were analyzed. The highest content of total phenolics, total flavonoids, total antioxidant capacity, procyanidin B2 and chlorogenic acid was measured in plums and blueberry bars. The highest level of reduced saccharides was evaluated in raspberry and cereal bars. Presence of main simple sugars - maltose, glucose and fructose was detected in all bar samples.
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Influence of unisex rates on the life insurance market
Vondráčková, Hana ; Řezanková, Hana (advisor) ; Zemánek, Martin (referee)
The diploma thesis deals with possible reaction of life insurance companies to legislative decree, in which the European Union forbid, within the equality of sexes, to use sex as a factor for assessing different amount of premium between men and women. Using analysis of the product pricing of five important insurance companies helps to show, which risks (and to which extent) this change covers. Because there will be limited possibility to decide on the premium amount according to one of the most important parameters, it can be expected, this decision will have significant influence on the whole insurance market and it will indicate direction of life insurance to the future. Decision about business strategy is influenced also by other entries, such as position of the insurance company, its business network etc. As the most likely direction of market development is here considered new or more distinctive segmentation of clients, hence there are, on accessible data, using the hypothesis about the relative frequency, tested propositions of the new criteria, which could help determine more fair premium.
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