|
|
Efect of the composition of an emulsion with imunosupressant on the rhelogical properities of the emulsion
Martinek, Vojtěch ; Šnejdrová, Eva (advisor) ; Vraníková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Author: Mgr. Vojtěch Martinek Title of thesis: Efect of the composition of an emulsion with imunosupressant on the rhelogical properities of the emulsion Supervisor: PharmDr. Eva Snejdrova, Ph.D. This thesis evaluates rheological properties of the emulsion created after mixing the drug product Cyclosporine Oral Solution, USP /MODIFIED/ 100 mg/ml with water in different ratios. The theoretical part describes general characteristics, stability and viscosity of emulsions. It also describes self-emulsifying systems and the tested product Cyclosporine Oral Solution, USP /MODIFIED/ 100 mg/ml. In the experimental part of the work, the type of emulsion created, its flow properties and the effect of homogenization intensity were characterized. It was experimentally verified that as the amount of water in the mixture increases, first a w/o emulsion is formed and then the emulsion turns to an o/w emulsion type. In terms of flow properties, the medicinal product exhibits Newtonian behavior. By gradually adding water, the behavior changes to non-Newtonian up to a water:product ratio of 70:30, where the minimum value of the flow behavior index is found. As water is further added, the properties change back to...
|
|
|
Adhesivity of in situ PLGA films for the local drug delivery.
Paňkiv, Julie ; Šnejdrová, Eva (advisor) ; Mužíková, Jitka (referee)
Charles University, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical technology Mentor: PharmDr. Eva Šnejdrová, Ph.D. Consultant: PharmDr. Věříš Andrea Student: Julie Paňkiv Title of Thesis: Adhesivity of in situ PLGA films for the local drug delivery The goal of this work was the formulation and study of the film forming systems FFS formed by poly(lactic-co-glycolic) acid of linear or branched structure, plasticized with methyl- salicylate, ethyl-pyruvate or ectoine, with incorporated cannabidiol. Acetone and ethyl acetate were tested as solvents. The theoretical part is focused on the characterization of FFS, their composition and testing. In situ films were characterized by DSC and SEM. The course of evaporation of the organic solvent from the FFS was monitored. A methodology for testing adhesion of in situ films using a tensile test on a rheometer was developed. It was found that the evaporation time of the organic solvent from FFS is influenced by both the type of solvent used and the type of plasticizer. In non-plasticized polymers, organic solvent evaporates faster than in plasticized ones. Evaporation of more than 90 % of the solvent occurs within 5 minutes. Compared to the commercial preparation Lamisil Once, the tested PLGA in situ films show higher adhesiveness....
|
|
|
Adhesivity of in situ PLGA films for the local drug delivery.
Paňkiv, Julie ; Šnejdrová, Eva (advisor) ; Mužíková, Jitka (referee)
Charles University, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical technology Mentor: PharmDr. Eva Šnejdrová, Ph.D. Consultant: PharmDr. Věříš Andrea Student: Julie Paňkiv Title of Thesis: Adhesivity of in situ PLGA films for the local drug delivery The goal of this work was the formulation and study of the film forming systems FFS formed by poly(lactic-co-glycolic) acid of linear or branched structure, plasticized with methyl- salicylate, ethyl-pyruvate or ectoine, with incorporated cannabidiol. Acetone and ethyl acetate were tested as solvents. The theoretical part is focused on the characterization of FFS, their composition and testing. In situ films were characterized by DSC and SEM. The course of evaporation of the organic solvent from the FFS was monitored. A methodology for testing adhesion of in situ films using a tensile test on a rheometer was developed. It was found that the evaporation time of the organic solvent from FFS is influenced by both the type of solvent used and the type of plasticizer. In non-plasticized polymers, organic solvent evaporates faster than in plasticized ones. Evaporation of more than 90 % of the solvent occurs within 5 minutes. Compared to the commercial preparation Lamisil Once, the tested PLGA in situ films show higher adhesiveness....
|
|
|
Formulation of vancomycin loaded PLGA nanoparticles for local treatment of musculoskeletal infections I.
Petrášová, Ivana ; Šnejdrová, Eva (advisor) ; Smékalová, Monika (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Author: Ivana Petrášová Title of thesis: Formulation of PLGA nanoparticles with vancomycin for local treatment of musculoskeletal infections I Supervisor: PharmDr. Eva Šnejdrová, Ph.D. The treatment of musculoskeletal infections requires the presence of high antibiotics concentration at the infection site. However, high doses of orally administered drugs cause the risk of increased systemic toxicity and serious adverse effects. Targeted drug delivery, currently one of the main areas of pharmaceutical research, represents a suitable option of a treatment without excessive burden on the organism. This diploma thesis focuses on the formulation of a therapeutic system for the local treatment of musculoskeletal infections in the form of polymer nanoparticles containing the antibiotic vancomycin. Due to the need of incorporation of a hydrophilic antimicrobial substance into them, the suspension-emulsion method of their preparation was used. Different concentrations of polyvinyl alcohol stabilizer and tripentaerythritol branched hydrophobic polymer poly(D,L- lactide-co-glycolide) were used for the preparation. Within the study the size of nanoparticles, polydispersity, zeta potential, encapsulation efficiency...
|
|
|
The preparation of tablets by selective laser sintering.
Ficek, Lukáš ; Mužíková, Jitka (advisor) ; Šnejdrová, Eva (referee)
Charles University, Faculty of pharmacy in Hradec Králové Department of: Pharmaceutical Technology Mentor: doc. PharmDr Jitka Mužíková, Ph. D Consultant: Mgr. Thao Tranová Student: Lukáš Ficek Title of Thesis: The preparation of tablets by laser sintering This work focuses on the preparation of orally dispersible tablets by selective laser sintering (SLS). The present project is a pilot study conducted on the 3D printer Sintratec Kit at the Department of Pharmaceutical Technology. SLS is a 3D printing method based on the fusion of powder particles using a laser beam that sinters the individual particles together in the layers of material.1-2 The matrix forming polymer used in work was Kollidon® VA64 which was combined with two pigments namely Candurin® Gold Sheen and NTX Ruby Red. In addition, mixtures of the polymer with a co-processed dry binder Prosolv® ODT G2 and a physical mixture of mannitol, silicified microcrystalline cellulose, and crospovidone at the concentrations of 20%, 40%, and 60% were used. The influence of printing parameters, especially laser speed, on tablet quality was tested. NTX Ruby Red pigment was selected for final tablet printing. The best powder formulation for optimal printing progress and high-quality properties of the orally dispersible tablets was the mixture of...
|
|
|
Formulation and characterization of w/o emulsions for local treatment of musculoskeletal infections
Vedralová, Štěpánka ; Šnejdrová, Eva (advisor) ; Mužíková, Jitka (referee)
CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical technology Author: Štěpánka Vedralová Title of diploma thesis: Formulation and characterization of w/o emulsions for local treatment of musculoskeletal infections Supervisor: PharmDr. Eva Šnejdrová, Ph.D. The aim of the diploma thesis was to formulate a depot dosage form for the prevention and local treatment of musculoskeletal infections. A water-in-oil emulsions have been formulated which, due to the partition coefficient between the outer and inner phases, can provide sustained drug release. Oils with different viscosities were tested as oil phases. Tricaprin was chosen for the preparation of emulsions, due to the highest stability of the prepared emulsion. Lecithin and sorbitan monooleate or polyglyceryl-3-polyricinooleate (PGPR) and magnesium stearate were used as emulsifiers. The aqueous phase was a solution of vancomycin hydrochloride and gentamicin sulphate. The flow properties on a rotational rheometer were evaluated. The influence of the emulsion composition and the homogenization method on the coefficient of consistency and the index of flow behaviour of the emulsions was studied. The emulsions stabilized with lecithin have a higher viscosity and a structure more sensitive to changes in composition...
|
|
|
Formulation and characterization of hydrogels for local treatment of musculoskeletal infections
Drdová, Pavlína ; Šnejdrová, Eva (advisor) ; Vraníková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Author: Pavlína Drdová Title of thesis: Formulation and characterization of hydrogels for local treatement of musculoskeletal infection Supervisor: PharmDr. Eva Šnejdrová, Ph.D. Local anti-infective therapy for musculoskeletal infections eliminates systemic drug toxicity. By selecting a suitable drug delivery system, higher drug concentrations at the site of infection and sustained release can be achieved. Such a system may be gels, formulated and tested in the submitted diploma thesis. The theoretical part of the work was devoted to the description of musculoskeletal infections and their treatment, characterization of gels and their use in parenteral administration. Hydrophilic gels based on hypromellose, carrageenan, gelatin and fish gelatin containing vamcomycin hydrochloride and gentamicin sulfate were formulated. Flow and viscoelastic properties were tested on a rotational rheometer. The analysis of flow curves by the Power law model proved a non-Newtonian type of flow. The most sensitive to applied stress is a 3 % hypromellose gel, the least a dispersion of iota-carrageenan with povidone 25. Viscoelastic properties were evaluated based on elastic, viscous and complex modulus, phase angle and yield...
|
|
|
Rheological properties of gelatin-based gels for ultrasound brain phantom
Bojňanská, Terézia Marianna ; Šnejdrová, Eva (advisor) ; Holas, Ondřej (referee)
CHARLES UNIVERSITY IN PRAGUE FACULTY OF PHARMACY IN HRADEC KRALOVE DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY Author: Terezia Marianna Bojnanska Supervisor: PharmDr. Eva Snejdrova, Ph.D. Title of diploma thesis: Rheological properties of gelatin-based gels for ultrasound brain phantom The diploma thesis deals with the evaluation of the viscoelastic properties of gelatin gels modified by addition of glutaraldehyde and glycerol using a rheometer with goal to verify the suitability of tested materials for their intended use as ultrasound brain phantoms. The theoretical part deals with gels in general and is focused on gelatin gels as a possible basic component of ultrasound phantoms, and the possibilities of characterizing gels as viscoelastic systems using methods of oscillatory rheometry. Gelatin gels of different concentrations (5; 7,5 and 10%) with the addition of glutaraldehyde and glycerol were characterized by complex modulus, complex viscosity, yield stress, phase angle. Temperature stability of the samples in the range from 20 to 50řC was tested. All tested gels were viscoelastic solids with a clearly defined linear-viscoelastic region, which is confirmed by phase angle values close to 0ř. With the increasing concentration of gelatin the values of the complex modulus increased, increasing...
|
|
|
Polymeric particles for targeted drug delivery into mononuclear phagocytic system
Prošková, Denisa ; Holas, Ondřej (advisor) ; Šnejdrová, Eva (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Supervisor: PharmDr. Ondřej Holas, Ph.D. Consultant: Dmytro Iefremenko, M.Sc. Student: Denisa Prošková Title of Thesis: Polymeric paticles for targeted drug delivery into mononuclear fagocytic system Polymeric nanoparticles (NPs) can act as drug nanocarriers. They are frequently used to enhance distribution to a target tissues in which they specifically act. The mononuclear phagocytic system (MPS) represents a target to which NPs could be specifically distributed. It has been found that cells of this system are involved in the development of a number of diseases. Optimal parameters of NPs suitable for MPS targeting are size ranging between 100 nm and 300 nm and negative surface charge. In this work the polymeric nanoparticles with encapsulated cholic acid were prepared by nanoprecipitation method using five selected types of non-ionic surfactants in concentration of 0,1%, 0,5%, 1% and 2%. PLGA was the polymer used for their preparation because of its high biocompatibility and biodegradability. Encapsulation efficiency, size and zeta-potential were selected as critical parameters chosen for prepared nanoparticles. The obtained data were used to characterize the effect of non-ionic surfactants on the...
|
|
|
A study of tableting materials and tablets with the retarding component containing hypromellose and carmellose sodium.
Koukolová, Kristýna ; Mužíková, Jitka (advisor) ; Šnejdrová, Eva (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Pharmaceutical Technology Student: Kristýna Koukolová Consultant: Assoc. Prof. PharmDr. Jitka Mužíková, Ph.D. Title of the Diploma Thesis: A study of tableting materials and tablets with the retarding component containing hypromellose and carmellose sodium The thesis deals with the study of compressibility of directly compressible tableting materials containing coprocessed dry binders MicroceLac® 100 and Prosolv® SMCC 90 in combination with the retarding component CompactCel® SR, which is a mixture of hypromellose and carmellose sodium. The tested concentrations of the retarding component were 10 %, 20 % and 30 %. The formulations also contained salicylic acid as a model drug at concentration of 25 % and sodium stearyl fumarate as a lubricant at concentration of 1%. Flow properties, specifically fowability, apparent volumes and densities were evaluated at the tableting materials. During tablet compression, compressibility was evaluated using the energy profile of the compression process. The tested tablet parameters were tensile strength, friability, disintegration and drug dissolution. Formulations with MicroceLac® 100 showed better flow properties. The addition of a retarding component worsened the flow properties. The values...
|
guest ::
