|
|
Linking transcriptional regulation of lipid metabolism with catastrophic mitosis in fission yeast
Marešová, Anna ; Převorovský, Martin (advisor) ; Čáp, Michal (referee) ; Veverka, Václav (referee)
Linking regulation of lipid metabolism with nuclear division has recently become a thoroughly investigated phenomenon. It has been shown that an undisturbed lipid metabolism is required for proper mitotic division from yeasts to mammals. In my doctoral project, I focused on the transcriptional regulation of lipid metabolism genes with respect to mitotic fidelity in the fission yeast Schizosaccharomyces pombe. Cbf11 and Mga2 are two transcriptional regulators of lipid metabolism genes. For a long time, it was assumed that they act as two independently functioning units. However, we have identified a novel regulatory partnership between Cbf11 and Mga2. Our results show that Cbf11 and Mga2 work closely together. In addition to regulating lipid metabolism, Cbf11 was known to be involved in cell cycle progression and a range of other processes. We have now determined that all known functions of Cbf11 require its canonical DNA-binding ability. Interestingly, we have identified novel roles for both Cbf11 and Mga2 and found that Mga2 mirrors the functions of Cbf11, including cell cycle control. Furthermore, we have shown that lipid metabolism has a pronounced impact on chromatin structure and the proper regulation of gene expression, and we have suggested how fatty acid synthesis contributes to mitotic...
|
|
|
Alphanumerical external wireless display device for PC
Veverka, Vítězslav ; Prokopec, Jan (referee) ; Beneš, Jan (advisor)
In this work was created the basic description and theoretical design of alphanumeric extern wireless module which could be connected to PC by the USB. This work includes the basic description of some alphanumerical visual modules connectable to PC, the charecterization of general used electrical networks and components, the basic parameters of high frequency modules and wireless transmision. On the basis of theoretical studies are described the oppotrunities of the project of complete alfanumerical extern wireless display and reasons of selected options. Next the creation of circuit diagrams and printed circuit boards. The result of this work is a functional prototype of the equipment including software for microprocessors and computer control. Verification of functionality and usability is part of the project.
|
|
| |
|
|
Automatic Delineation of Multi-lead ECG Signals
Veverka, Vojtěch ; Smital, Lukáš (referee) ; Hejč, Jakub (advisor)
This semester thesis is focused on automated measurement of ECG signal. The theoretical part describes the rise and options ECG signal. Furthermore, the issue is staged principal components analysis, whose output is used as input signal for seasons. They describe the basic methods used in measurement to ECG signal. The practical part is designed in measurement algorithm for ECG signal that has been tested on basic CSE database. The results are discussed in the conclusion.
|
|
|
Software for QRS detectors performance evaluation
Veverka, Vojtěch ; Vítek, Martin (referee) ; Ronzhina, Marina (advisor)
QRS complex is the most prominent part of ECG signal. Due to its properties, it is used in calculation of heart rate and serves as one of the reference points for automatic delineation of ECG. Therefore, the accurate detection of QRS complexes is very important. This work is focused on creating of software for evaluation of different QRS detection approaches. The first part includes the basics of electrocardiography, the most common methods for QRS detection and also various artifacts that can affect the detection, The practical part is focused on the development of some detectors and the software itself. The detectors are then tested with software using the CSE database of real ECG recordings.
|
|
|
Alphanumerical external wireless display device for PC
Veverka, Vítězslav ; Frýza, Tomáš (referee) ; Prokopec, Jan (advisor)
The target of this work was creation of alphanumeric extern wireless module which could be connected to PC by the USB and the realization of this project. This work includes the basic description of some alphanumerical visual modules connectable to PC, the characterization of general used electrical networks and components, the basic parameters of high frequency modules and wireless transmision. On the basis of theoretical analysis are described the oppotrunities of the project of complete alfanumerical extern wireless display and reasons of selected options. Next the creation of circuit diagrams and printed circuit boards. The result of this work is a functional prototype of the equipment including software for microprocessors and computer control. Verification of functionality and usability is part of the project.
|
|
|
RC car model
Veverka, Vítězslav ; Frýza, Tomáš (referee) ; Šebesta, Jiří (advisor)
The aim of this bachelor thesis was an analysis of possibilities and requirements for driving systems of vehicle model. In the thesis, available undercarriages, which are suitable for an implementation of driving system, were presented. Furthermore, the study of the interface for the regulator and possibility to use a microcontroller in the driving system, were necessary. The wireless communication between transmitting part and microcontroller was tested just as controlling of an engine speed. The programme for driving of the model in PC was designed and tested by using C language. General behaviour of the model was tested. The response to control and achieved driving precision was tested too. In the end, assistant sensors for collision detection were added to the model.
|
|
|
Receiver for remote control
Veverka, Vojtěch ; Horák, Martin (referee) ; Kolka, Zdeněk (advisor)
This semestral project deals with the plan of peripheral involvement of the receiver remote controller, which will serve as a control module for controlling the turning antenna rotator. Part of the work is focused on the issues of wireless remote controllers, the modulation method used for transmitted order and transmission protocols. Then there is solved the problem of the right choice microcontroller for controling the entire application, including a description of its basic properties. The work also solves the overall interconnection of control units, power supplies and antenna rotator itself.
|
|
|
Destruktivní metody testování materiálu
Veverka, Vilém
The submitted bachelor's thesis deals with destructive methods of material testing with a focus on tensile testing and material fatigue. The aim of the bachelor's thesis was to prepare an overview of individual destructive methods of material testing and comparison of tensile diagrams of samples. In the bachelor's thesis, 3 types of samples were investigated, which were printed on a Prusa i3 MK3s 3D printer from ABS, PLA and PETG materials. Test specimens were modeled in Fusion 360 according to ASTM D638. The tensile test was performed on a ZDM 5/51 universal tensile machine according to the ASTM D638 standard. The results show that the sample made of PLA material has the highest tensile strength of 41,23 MPa.
|
|
|
Capturing chromatin-associated proteins in nucleosomal context
Koutná, Eliška ; Veverka, Václav (advisor) ; Hlouchová, Klára (referee) ; Bařinka, Cyril (referee)
| 9 ABSTRACT Eukaryotic DNA is stored in the nucleus wrapped around histone octamers in the form of nucleosomes. These basic chromatin units can further associate with DNA-binding factors through DNA and the N-terminal histone tails that are subjected to various covalent posttranslational modifications that, in combination with DNA modifications, define the epigenetic code. Eukaryotic transcription is dependent on these specific histone modifications - their recognition by chromatin reader proteins triggers complex processes relying on the coordinated association of transcription regulatory factors. Although various modification states of a particular histone residue often lead to differential outcomes, it is not entirely clear how they are discriminated at a molecular level. Moreover, the contribution of intrinsically disordered regions outside of the specialized reader domains to nucleosome binding remains unexplored. In this thesis, the main focus is put on the transcription coactivator LEDGF/p75, capable of reading the H3K36me2/3 histone mark, and the pioneer transcription factor Sox2. Using structural biological and biophysical techniques, the interaction of LEDGF and Sox2 with nucleosomes is dissected, describing the peculiarities of intrinsically disordered DNA interacting motifs. Two...
|
guest ::
RSS feed.