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Development and validation of UHPLC-MS/MS method for determination of urea in exhalted breath condensate and its application in clinical research
Vrbová, Lenka ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Lenka Vrbová Supervisor: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Consultant: PharmDr. Kateřina Matoušová, Ph.D. Title of the diploma thesis: Development and validation of UHPLC-MS/MS method for determination of urea in exhaled breath condensate and its application in clinical research Key words: urea, exhaled breath condensate, UHPLC-MS/MS Exhaled breath condensate analysis is a simple non-invasive method of measuring biomarkers not only from the respiratory tract. For the proper clinical interpretation of the measured data it is necessary to find the dilution biomarker due to dilution of target analytes with condensed water vapour. Urea seems to be the molecule of choice. The aim of this diploma thesis was to develop a fast, simple and sensitive analytical method for determining urea concentration levels in exhaled breath condensate for clinical research. Ultra-high performance liquid chromatography with mass spectrometry detection was chosen for urea analysis. Kinetex HILIC 100 A column, 1.7 µm particle size, 100 × 2.10 mm (Phenomenex, USA) was used for the separation. The mobile phase was composed of acetonitrile, methanol and water in a ratio of 81 : 9 : 10, the organic part was...
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Maintaining ergotherapy as free time activities male clients in retirements homes
VRBOVÁ, Lenka
The thesis is divided into a theoretical and an empirical part. The theoretical one explains the position of fitness ergotherapy among leisure activities. The aim of the qualitative survey of the empirical part is to figure out how ergotherapy fulfils the needs of home's clients, especially men - how they engage in the workshop activities. The first chapter describes the concepts of aging and old age, it focuses on the needs of seniors. The second chapter describes the home for the elderly and possibilities of leisure activities. The third chapter deals with the concept of ergotherapy. It focuses on fitness ergotherapy and its use in such facilities. The main needs fulfiled through the activities in the men's workshop are apparently socializing, life goal, stimulation, selfesteem, life continuity and emotional relationship among the clients.
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Media representation of muslim women in mlada fronta dnes, and its development in time
Poulová, Radka ; Macků, Lucie (advisor) ; Vrbová, Lenka (referee)
Though the objective of news reporting in mass media is to retain neutrality, the reality has showed us that this aim is never completely fulfilled: several factors enter the news creating process which result in the final picture being deformed and misrepresentations and stereotyping are created. In the following text, we first outline the basics of these mechanisms, as well as the basic characteristic of islam and the position of women in it. Later on, we look particularly at how the media portrayals of muslim women are being created. We analyse the portrayal of muslim women in Mladá fronta DNES, the largest Czech national serious daily paper, during the course of the years 2001 and 2011. We try to figure out what a common perception of a muslim woman in the news stories is, and in which areas the muslim women are missing. We use both quantitative and qualitative analysis and focus on both written materials and pictures.
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Gender sensitive parenting
Vrbová, Lenka ; Smetáčková, Irena (advisor) ; Kiczková, Zuzana (referee)
This thesis follows an issue of gender sensitive parentage. In particular to parents who decided to educate their kids gender sensitively. In the first part it introduces theories of gender socialization and acquiring of gender identity. Further it introduces socialization in family and, in relation it, several social factors. It tries to approach this issue through gender sensitive education. This thesis also takes a deep look to existing approaches and researches of gender sensitive education. The research part of the thesis is based on qualitative analysis of interviews with parents who are trying to educate gender sensitively. This analysis detects how these parents comprehend gender sensitive education, how they make it work, what strategies they bring up, to what situations they get while following this education program and what obstacles they face up. The goal of this dissertation is to transmit the living experience of people who try to educate their children gender sensitively and contribute the development of possibilities allowing free progression to their kids.
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Changes in tax administration in relation to the adoption of the Tax Procedure Code
Vrbová, Lenka ; Vondráčková, Pavlína (advisor) ; Novotný, Petr (referee)
75 Abstract - Changes in tax administration in relation to the adoption of the Tax Procedure Code For my diploma thesis I have chosen the topic which I consider as very actual currently and therefore it needs attention. The purpose of my thesis named "Changes in tax administration in relation to the adoption of the Tax Procedure Code" is to analyze the selected issues of Act no. 337/1992 Sb., about administration of taxes and fees (hereinafter the ZSDP) and refers to judgments of the Supreme Administrative Court and the Constitutional Court of the Czech Republic and then compares these problematic issues with Act no. 280/2009 Sb., Tax Procedure Code, which is going to come into force on 1. January 2011. The topic I am interested in is very huge, so I do not deal with all changes in tax administration that Tax Procedure Code provides. My thesis focuses only on those areas of tax administration, which are essential for the process of tax administrators and for negotiation of taxpayers. In particular, I focus on those areas of legislation in which Tax Procedure Code establishes the rights and responsibilities that were previously inferred only from the case of the Czech court. My thesis is formally composed of five chapters, these chapters are divided into subchapters and sections. The structure is organized...
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Vliv lepidla na pevnost lepených spojů za zvýšených teplot
Vrbová, Lenka
The goal of this thesis is an analysis of the influence of the adhesive material on the characteristic strength of the adhesive bonded joint. The adhesive material used was exposed to the cyclic thermal loading of the environment around the adhesive bonded joint of ABS and PB furniture edges. The solidity of adhesive bonded joint was tested by loading of the joint, which was achieved by peeling of the ABS edge at 90° angle. Only standard materials, commonly accessible and utilised in the furniture industry, were used during the test. The results of the gauging were processed and analysed.
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