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Visions for Krnov
Moravcová, Sofia ; Mašek, Radomír (referee) ; Marek, Jiří (advisor)
The Library is a place, which convey knowledge and information to the general public. It should be a place for a person to immerse oneself, but also provide space for communities. The library building should be representative and welcoming institution, which has an important role within the city and the events taking place in it. With these things in mind I proceeded to design this building.
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Memory overflow
Moravcová, Sofia ; Hlavičková, Kateřina (referee) ; Mléčka, Jan (advisor)
Since Japanese architecture is deeply rooted in a place, the right choice of location was very important. It had to be a place that I know very well and spent a lot of time there in the past. I have experienced it over the years and seasons. When considering the location, I also thought about what I want to create there. In my pre-diploma, I analyzed the basic building types for Japanese architecture: house, tea houe, shrine and temple. With that naturally came the question of how to adapt these forms in our country without creating ordinary copies or having to mark these buildings. How do those forms differ from our counterparts? What shaped them? Of course, the different environment and technical requirements for buildings (high humidity, frequent earthquakes) played an important role, but ritual was one of the essential aspects. The Japanese value norms and rules very much. This means that customs were an integral part, not only of architecture. The tea house was created by adapting to the needs of the tea ceremony. The Shinto temple emphasizes the sacred way to the object through the torii gate, rinsing the hands and mouth - just like in the tea ceremony, donating a gift to the temple (a few small coins) and saying a prayer. The house itself has a correlation with several rituals of everyday life, from ikebana, through the preparation of meals to cleansing the body. The motif of purification is central to all three forms. To clarify, a Japanese bath works like this: first you rinse your body outside the bath with a shower. Then you enter the bath, which is only for relaxation. It is important to enter the bathtub completely clean, because the whole family will use the bathtub for one fill during the evening. This kind of ritual is not limited only to households. Japan is a country rich in thermal springs, the so-called onsens. Many of them, as part of accommodation facilities, are a very popular place for regeneration. Slovakia, as a much smaller country, also has considerable thermal wealth. Most of them are used commercially in our country. In the place near the village of Kalameny under the Chočské vrchmi, where I spent my holidays since childhood, the place where my grandparents come from, one of these springs springs up. The famous thermal baths Lúčky and Liptovský hrad are located nearby. The goal of this work is to implement spatial forms in the given location inspired by the ritual of tea, the ritual of purification and the visit to the temple, as well as the connection of connections between “European” and “Japanese”, which have not yet been connected.
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Internal communication within the circadian system and its significance for our health
Honzlová, Petra ; Sumová, Alena (advisor) ; Moravcová, Simona (referee)
Mammalian circadian cycle is generated by hierarchically organized system of internal rhythmical oscillations in clock gene expression (Clock, Bmal1, Per, Cry, Rev-Erb, etc.) which take place in nearly all living cells in our body. The master pacemaker is located in suprachiasmatic nucleus (SCN) in hypothalamus. According to its synchronization to photic and non-photic external stimuli SCN generates signal for entrainment of peripheral clock. Peripheral clock synchronization is maintained via neuronal or hormonal (glucocorticoids, melatonin) pathways, regulation of body temperature or food intake and affects various physiological processes. Desynchronization of central and peripheral clock can be the cause or the manifestation of impaired health condition. Powered by TCPDF (www.tcpdf.org)
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Role of apoptosis in the mechanism of opioid effect
Moravcová, Simona ; Novotný, Jiří (advisor) ; Ostašov, Pavel (referee)
2. Abstract Opioids have been used as effective analgesics for many years. The worst problem is, however, their adverse effects and potential addiction. There are three known types of opioid receptors that differ mainly in their sensitivity to different opioids and their distribution in the CNS. This work also deals with apoptosis. Apoptosis is an important process not just in the embryonic development, e.g. for the right differentation of fingers and toes, but also in the adulthood, e.g. to suppress tumour formation. Whereas an apoptotic pathway can be initiated in many different ways, there is only one mechanism that actually causes the death of a cell. It is important for the cell death and division to be in balance. The breach of this balance may result in pathologic effects. This work summarizes the effects of opioids in connection with apoptosis. The main object of the research of this relationship was morphine which has both pro-apoptotic and anti-apoptotic effects. Another two frequently investigated opioids are methadone and heroin. Heroin is mainly used in the studies researching the impact of this highly addictive opioid on the apoptosis and embryonic development. Keywords: opioids, opioid receptors, G-proteins, apoptosis, morphine
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Regulation of the expression of MHC class I molecules and other immunoactive molecules on tumor cells
Moravcová, Simona ; Reiniš, Milan (advisor) ; Vopálenský, Václav (referee)
4 Abstract Regulation of the expression of MHC class I molecules and other immunoactive molecules on tumor cells The aim of this master thesis project was to characterize the effects of IFNγ, TNFα and of the epigenetic agents 5-azacytidine (5AC) and 5-aza-2'-deoxycytidine (5AZAD) on the expression of molecules important for antigen presentation and modulation of the immune responses against tumors (MHC class I molecules and other immunoactive molecules CD54, CD80, B7-H1, B7-H2 and CD1d) on tumor cells. The experimental model used for this purpose were the HPV16-associated murine tumor cell lines, either MHC class I positive or negative. The goal was to determine the changes in surface expression of these molecules after treatment by FACS studies and also the expression at the mRNA level using qPCR. Expression of these proteins was also compared in the experiments in vitro on tumor cell lines and ex vivo on tumor cells explanted from animals. The most interesting result is the observation that 5AZAD increases the expression of B7-H1 which inhibits T cell mediated immune response and that 5AZAD and IFNγ act synergistically in the induction of the expression of MHC class I, CD54 and B7-H1 molecules. Klíčová slova: imunoeditace, protinádorová imunita, HVP16, MHC glykoproteiny I. třídy, B7-H1, IFNγ, TNFα,...
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Modulation of the JAK/STAT signaling pathway in the suprachiasmatic nucleus of rat hypothalamus
Moravcová, Simona ; Novotný, Jiří (advisor) ; Krulová, Magdaléna (referee) ; Polidarová, Lenka (referee)
Circadian clock in the suprachiasmatic nucleus of the hypothalamus (SCN) regulates daily rhythms in behavior and physiology and is an important part of the mechanisms regulating mammalian homeostasis. SCN are synchronized with a 24hour cycle mainly by light, but they can also be regulated by a variety of nonphotic signals, such as growth factors, opioids, cytokines, or lipopolysaccharide (LPS), which act by inducing the JAK/STAT signaling pathway. STAT family proteins (i.e. signal transducers and activator of transcription) regulate many aspects of cellular physiology, from growth and differentiation to immune response. However, the JAK/STAT signaling pathway has not yet been studied in the SCN and the function of STAT proteins in the SCN has not yet been clarified. In the first part of the thesis, we focused on localization of STAT3 and STAT5 proteins in the rat SCN and determination of rhythm in proteins and mRNA. Our experiments showed the daily rhythm in the levels of STAT3 protein in SCN astrocytes of rat with low but significant amplitude and with maximum in the morning. In addition, we revealed strong but nonrhythmic expression of STAT5A protein in astrocytes and STAT5B protein in nonastrocytic cells of SCN. It was also found that Stat3 mRNA show, similarly to protein, circadian rhythm in...
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Visions for Krnov
Moravcová, Sofia ; Mašek, Radomír (referee) ; Marek, Jiří (advisor)
The Library is a place, which convey knowledge and information to the general public. It should be a place for a person to immerse oneself, but also provide space for communities. The library building should be representative and welcoming institution, which has an important role within the city and the events taking place in it. With these things in mind I proceeded to design this building.
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The effect of melatonin on the immune system
Hrubcová, Leona ; Moravcová, Simona (advisor) ; Boháčová, Pavla (referee)
Melatonin is an important hormone which is known to have very diverse functions. It was originally discovered as a product of the pineal gland synthesized in a 24 hour rhythm, but in later studies was found to be synthesized in many different tissue types. Melatonin is an important part of the circadian system and its effects on sleep rhythm are well known. The effects of melatonin on the circadian system are briefly covered in the opening chapters of this thesis. Furthermore, the basic mechanism of inflammation and the diverse effects of melatonin on the immune system are described in this thesis. Melatonin acts in an anti-inflammatory as well as pro-inflammatory manner and is part of many research projects focusing on curing for example diseases associated with chronic inflammation. This thesis presents studies regarding the effects of melatonin on pathological conditions like neurodegenerative diseases, rheumatoid arthritis and sepsis. This thesis also describes how changes in immune system activity can change the expression of melatonin, as it is not a one way effect. However, this interaction is not well known yet. Key words: melatonin, circadian system, immune system, inflammation
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Default Mode Network: Ontogeny
Jarmar, Jiří Kryštof ; Nekovářová, Tereza (advisor) ; Moravcová, Simona (referee)
Default mode network is a functional network system in a brain, comprising of other sub- components. It is activated during tasks focused on the inner monologue, mentalization, episodical memory or self-reflection. The DMN activity is generally described as default mode functioning of the brain in absence of external stimuli, on the other hand its activity is inhibited during the presence of it. This thesis focuses on the main factors and changes that occur during ontogeny of DMN from birth to adulthood, mainly in structural and functional connectivity and anticorrelation relationships with other brain networks. After a general characterization of DMN, the first part focuses on general characterization of functional brain networks and statistical methodology of measuring their relationships and maturity. It follows by the description of important events in brain development and identification of main anatomical regions of DMN. The most important part is the last one, which provides observations of comparative studies of DMN between children and adults. Here we describe main changes and trends in ontogeny of DMN. Key words: Default mode network, functional network, fMRI, ontogeny
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