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Separation and determination of isoelectric points of GnRH peptides by capillary electrophoresis in conventional and carrier ampholytes-based background electrolytes
Šolínová, Veronika ; Poitevin, M. ; Barth, Tomislav ; Peltre, G. ; Kašička, Václav
Two mixtures of human and salmon gonadotropin releasing hormone (GnRH) derived peptides were successfully separated by capillary zone electrophoresis (CZE) in six classical buffer-based BGEs and by carrier ampholytes-based capillary electrophoresis (CABCE) in the same number carrier ampholytes-based isoelectric BGEs of the same or close pH values. In addition, from the pH dependence of peptide electrophoretic mobilities, the isolectric points of separated peptides were determined.
Separation and characterization of GnRHs by capillary electrophoresis in classical and isoelectric buffers
Šolínová, Veronika ; Poitevin, M. ; Barth, Tomislav ; Peltre, G. ; Kašička, Václav
Capillary zone electrophoresis (CZE) in classical buffer-based background electrolytes and carrier ampholytes-based capillary electrophoresis (CABCE) using isoelectric BGEs (narrow pH fractions of carrier ampholytes used in isoelectric focusing) have been applied to separation and characterization of synthetic human gonadotropin-releasing hormone (hGnRH) and its fragments. From the measured effective mobilities of these peptides within a broad pH range (3.0-10.0), their isoelectric points (pI) were determined.
Příprava a charakterizace [Glu]n peptidů
Šolínová, Veronika ; Přibylová, Marie ; Kašička, Václav ; Vaněk, Tomáš ; Barthová, J. ; Barth, Tomislav ; Lepša, L.
Several oligomers of L-glutamic acid (n = 2-10) were synthesized by a solid phase method, analyzed and separated by capillary zone electrophoresis (CZE) as anions in alkaline background electrolytes of different composition at pH range 8.1-10.0.
Predikce tvaru molekul a separace strukturně příbuzných hormonů uvolňujících gonadotropin (GnRH) kapilární elektroforézou
Šolínová, Veronika ; Kašička, Václav ; Sázelová, Petra ; Barth, Tomislav
Semiempirical models of the dependence of effective mobility of peptides on the ratio of their charge and relative molecular mass were tested to describe the electromigration behavior of peptides, particularly the shape of their molecules.
Užití kapilární zónové elektroforézy a izotachoforézy k vyhodnocení stupně čistoty a obsahu protiiontů v preparátech lecirelinu
Sázelová, Petra ; Kašička, Václav ; Šolínová, Veronika ; Flegel, Martin ; Barth, Tomislav
Different batches of lecirelin, analogue of LH-RH, were checked for purity.. The mass percentages of contaminating compounds were determined by CITP in anionic mode.
Induced ovulation and artificial propagation of barbel (Barbus barbus) females using different doses of GnRH analogue
Kouřil, J. ; Hájek, J. ; Barth, Tomislav
An experiment was conducted to induce ovulation in barbel (Barbus barbus) originated from river by a single injection of preparate Supergestran (contains GnRH analogue Lecirelin) in three different dosis and carp pituitary (control group).

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