Home > Conference materials > Papers > Separation and determination of isoelectric points of GnRH peptides by capillary electrophoresis in conventional and carrier ampholytes-based background electrolytes
Original title:
Separation and determination of isoelectric points of GnRH peptides by capillary electrophoresis in conventional and carrier ampholytes-based background electrolytes
Authors:
Šolínová, Veronika ; Poitevin, M. ; Barth, Tomislav ; Peltre, G. ; Kašička, Václav Document type: Papers Conference/Event: International Symposium Advances in Chromatography and Electrophoresis and Chiranal, Olomouc (CZ), 2012-06-11 / 2012-06-14
Year:
2012
Language:
eng Abstract:
Two mixtures of human and salmon gonadotropin releasing hormone (GnRH) derived peptides were successfully separated by capillary zone electrophoresis (CZE) in six classical buffer-based BGEs and by carrier ampholytes-based capillary electrophoresis (CABCE) in the same number carrier ampholytes-based isoelectric BGEs of the same or close pH values. In addition, from the pH dependence of peptide electrophoretic mobilities, the isolectric points of separated peptides were determined.
Keywords:
capillary zone electrophoresis; carrier ampholytes; GnRH peptides Project no.: GA203/08/1428 (CEP), GAP206/12/0453 (CEP) Funding provider: GA ČR, GA ČR Host item entry: Acta Universitatis Palackianae Olomucensis. Facultas Rerum Naturalium. Chemica 50S, ISBN 978-80-244-3115-4, ISSN 0232-0061
Institution: Institute of Organic Chemistry and Biochemistry AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0216668