Národní úložiště šedé literatury Nalezeno 3 záznamů.  Hledání trvalo 0.00 vteřin. 
Separation and determination of isoelectric points of GnRH peptides by capillary electrophoresis in conventional and carrier ampholytes-based background electrolytes
Šolínová, Veronika ; Poitevin, M. ; Barth, Tomislav ; Peltre, G. ; Kašička, Václav
Two mixtures of human and salmon gonadotropin releasing hormone (GnRH) derived peptides were successfully separated by capillary zone electrophoresis (CZE) in six classical buffer-based BGEs and by carrier ampholytes-based capillary electrophoresis (CABCE) in the same number carrier ampholytes-based isoelectric BGEs of the same or close pH values. In addition, from the pH dependence of peptide electrophoretic mobilities, the isolectric points of separated peptides were determined.
Capillary zone electrophoresis in strongly acidic/basic classical and (quasi)isoelectric background electrolytes applied to separation and characterization of biopeptides
Kašička, Václav ; Koval, Dušan ; Šolínová, Veronika ; Busnel, J. M. ; Poitevin, M. ; Peltre, G.
Capillary zone electrophoresis (CZE) in classical buffer-based background electrolytes (BGEs) or in isoelectric BGEs and carrier ampholytes-based capillary electrophoresis (CABCE), which uses narrow pH cuts of carrier ampholytes (CAs) as quasi-isoelectric BGEs, are presented as a powerful techniques for separation and physicochemical characterization of biologically active peptides.
Separation and characterization of GnRHs by capillary electrophoresis in classical and isoelectric buffers
Šolínová, Veronika ; Poitevin, M. ; Barth, Tomislav ; Peltre, G. ; Kašička, Václav
Capillary zone electrophoresis (CZE) in classical buffer-based background electrolytes and carrier ampholytes-based capillary electrophoresis (CABCE) using isoelectric BGEs (narrow pH fractions of carrier ampholytes used in isoelectric focusing) have been applied to separation and characterization of synthetic human gonadotropin-releasing hormone (hGnRH) and its fragments. From the measured effective mobilities of these peptides within a broad pH range (3.0-10.0), their isoelectric points (pI) were determined.

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