Original title: Separation and characterization of GnRHs by capillary electrophoresis in classical and isoelectric buffers
Authors: Šolínová, Veronika ; Poitevin, M. ; Barth, Tomislav ; Peltre, G. ; Kašička, Václav
Document type: Papers
Conference/Event: Biologically Active Peptides. Conference /11./, Praha (CZ), 2009-04-22 / 2009-04-24
Year: 2009
Language: eng
Abstract: Capillary zone electrophoresis (CZE) in classical buffer-based background electrolytes and carrier ampholytes-based capillary electrophoresis (CABCE) using isoelectric BGEs (narrow pH fractions of carrier ampholytes used in isoelectric focusing) have been applied to separation and characterization of synthetic human gonadotropin-releasing hormone (hGnRH) and its fragments. From the measured effective mobilities of these peptides within a broad pH range (3.0-10.0), their isoelectric points (pI) were determined.
Keywords: capillary zone electrophoresis; GnRH; peptides
Project no.: CEZ:AV0Z40550506 (CEP), GA203/08/1428 (CEP), GA203/09/0675 (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: Biologically Active Peptides. 11th Conference, ISBN 978-80-86241-31-9

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0177754

Permalink: http://www.nusl.cz/ntk/nusl-40733


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers