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Proposal of a marketing strategy for a start-up
Živná, Lucie ; Kaňovská, Lucie (referee) ; Havíř, David (advisor)
This master thesis focuses on the design of a marketing strategy for a service start-up. It is divided into three parts. The theoretical part explains the theoretical concepts describing marketing strategy and digital marketing. The second part focuses on the analysis of the marketing mix, the external environment and the SWOT analysis. Through interviews, the interest in the company's platform is investigated. The last part consists of custom solution proposals outlining a new marketing strategy for the company including a timeline.
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Purification and characterization of selected human microsomal reductase
Skarka, Adam ; Wsól, Vladimír (advisor) ; Živná, Lucie (referee)
Area of human liver microsomal reductases hasn't been fully explored yet. Purification and characterization of still undescribed enzymes is an important step in finishing of this task. There is separation and purification of this reductases described in my work, with usage of hydrophobic interaction chromatography on low pressure chromatography instrument ÄKTA purifier. There were two columns with different substrates used, Phenyl sepharose (low sub) and Octyl sepharose. Separation conditions were the same in both cases. Results obtained by incubation of fractions and measurement on HPLC showed significant differences between both columns and their substrates. But it isn't possible to determine, which column is better for purification, because big area still remain for adjustment and optimization of purification conditions.
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Hygienic problematics of forest kindergartens in comparison with classical kindergartens
ŽIVNÁ, Lucie
This work, which is named "Hygienic problematics of forest kindergarten in comparison with classical kindergartens", is about differences between two types of preschool educational facilities. The theoretical part introduces with creation of classical and forest kindergartens, explains the importance of preschool educational facilities and the environment in childrens development, summarizes the current state of hygienic law regulations in the field of preschool education, summarizes hygienic requirements for places, service and catering, and last, but not least, points out health risks which are omnipresent. The aim of the work is comparison of classical and forest kindergartens in terms of their impact on health of children and their morbidity, as well as hygienic requirements for places, services, conditions and catering, and the comparison of differences in educational activities. The research questions are as follows: Does the type of kindergarten affect the health and morbidity of children? What are the hygienic risks of catering? Are the different hygienic conditions reflected in the service of kindergartens? What are the differences in educational activities? The research was conducted qualitatively with semistructured interview with teachers, and with observation in research file. The research file was composed of five kindergartens in the Pilsen Region three classical and two forest kindergartens (at that time forest clubs, which are planning to enter the school register and then, they'll be officially forest kindergarten). Those preschool facilities was randomly chosen. From the acquired interview data were created diagrams in categories, with using of methond called "paper and pencil", and individual subcategories were then verbally described and the answers of probands were compared. From the observing were, according to categories, verbally compared all of the researched facilities. The results show that the type of facility doesn't have any significant effect on the health and morbidity of children, but in case of some bigger trauma would be forest kindergartens in advantage thanks to handy first aid kit. Catering in forest kindergarten doesn't have sufficient legislative regulation and therefore there are unresolved questions about monitoring of nutritional recommendations, consumer basket and storage of childrens home made snacks. Forest kindergartens should be able to provide a daily catering for children, which consists of two snacks and a lunch, nonetheless, this is not the case of forest clubs, but after they enter the school register they'll have to fulfill that. Next thing that came out from the results is that there are large differences in area options of facilities, water supply, in hygienic equipment and in a way of securing of hygiene supplies. In terms of educational activities forest kindergartens are able to show children a manly part of education, on top of that they care about natural development of children and it's imagination, due to elementary natural materials. Moreover children in forest kindergartens spent three times more time outdoors that children in classical kindergartens.
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Purification and characterization of selected human microsomal reductase
Skarka, Adam ; Wsól, Vladimír (advisor) ; Živná, Lucie (referee)
Area of human liver microsomal reductases hasn't been fully explored yet. Purification and characterization of still undescribed enzymes is an important step in finishing of this task. There is separation and purification of this reductases described in my work, with usage of hydrophobic interaction chromatography on low pressure chromatography instrument ÄKTA purifier. There were two columns with different substrates used, Phenyl sepharose (low sub) and Octyl sepharose. Separation conditions were the same in both cases. Results obtained by incubation of fractions and measurement on HPLC showed significant differences between both columns and their substrates. But it isn't possible to determine, which column is better for purification, because big area still remain for adjustment and optimization of purification conditions.
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