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Polymeric delivery systems for immunooncotherapy
Šírová, Kateřina ; Etrych, Tomáš (advisor) ; Konvalinka, Jan (referee)
Oncological diseases are among the most common causes of death in developed countries and for some of them, current medicine still does not have satisfactory results. Designing of polymer therapeutics characterized by high molecular weight and controlled drug delivery is one of the perspective ways of development of new therapeutic protocols. Nowadays, scientists also focus on the research on anticancer immunological mechanisms and their potential use in cancer therapy. Constitutive activation of signal transducer and activator of transcription 3 (STAT3) has been described in many types of malignancies. The function of STAT3 is very complex, the aberrant activation in cells of tumor microenvironment is related to many predominantly prooncogenic processes. This bachelor thesis is focused on synthesis of peptide inhibitor of STAT3 signaling, phosphopeptide pYLPQTV, and its conjugates with polymer carrier based on N-(2-hydroxypropyl)methacrylamide (HPMA). The inhibitor and the polymeric conjugates have been characterized by several physico-chemical techniques; also, effect on proliferation of cancer cells have been tested in in vitro conditions, which proved slightly cytotoxic activity. A more detailed study and an optimization of methods for in vitro characterization will be necessary to further...
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The Pedagogy of Experience and Jabok Introductory Course 2005
Šírová, Kateřina ; Ortová, Marie (advisor) ; Růžička, Vít (referee)
This final work deals with outdoor pedagogy and introduced workshop of Jabok in 2005. First there is general paper on experience and outdoor pedagogy. Then there is general description outdoor workshop (how prepore, do and appraise this workshop). The finally there is presentation of introduced workshop of Jabok in 2005 (where is mainly appraise this workshop). Powered by TCPDF (www.tcpdf.org)
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Activities of enzymes involved in transformation of polycyclic aromatic hydrocarbons during composting
Šírová, Kateřina ; Cajthaml, Tomáš (advisor) ; Dračínská, Helena (referee)
Polycyclic aromatic hydrocarbons (PAHs) are recalcitrant organic pollutants, which occur widely in the environment. Some of these compounds are carcinogenic and toxic, many studies therefore focus on suitable remediation technologies. It has been shown that composting is an efficient treatment for contaminated solid matrices. Changes in several enzyme activities during co-composting of PAH-contaminated soil were studied in this thesis. The total initial concentration of analyzed PAHs in the soil was 1065 ± 86 µg·g-1 . The chosen activities represented well-known key enzymes involved in the transformation of PAHs or catechol as the central metabolite of PAH microbial degradation. At first, a method for extraction of the selected enzymes from the compost matrix was optimized. This approach was then used for the extraction of the enzymes from compost samples collected at each phase of composting. The activity of manganese peroxidase, laccase, tyrosinase and catechol-2,3-dioxygenase was detected during the cooling and the maturation phase. The only detected activity during the initial mesophilic phase was that of manganese peroxidase. The activities of catechol-1,2- dioxygenase and lignin peroxidase were not detected at all. Despite the fact that PAHs were substantially degraded, no influence of PAHs...
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Polymeric delivery systems for immunooncotherapy
Šírová, Kateřina ; Etrych, Tomáš (advisor) ; Konvalinka, Jan (referee)
Oncological diseases are among the most common causes of death in developed countries and for some of them, current medicine still does not have satisfactory results. Designing of polymer therapeutics characterized by high molecular weight and controlled drug delivery is one of the perspective ways of development of new therapeutic protocols. Nowadays, scientists also focus on the research on anticancer immunological mechanisms and their potential use in cancer therapy. Constitutive activation of signal transducer and activator of transcription 3 (STAT3) has been described in many types of malignancies. The function of STAT3 is very complex, the aberrant activation in cells of tumor microenvironment is related to many predominantly prooncogenic processes. This bachelor thesis is focused on synthesis of peptide inhibitor of STAT3 signaling, phosphopeptide pYLPQTV, and its conjugates with polymer carrier based on N-(2-hydroxypropyl)methacrylamide (HPMA). The inhibitor and the polymeric conjugates have been characterized by several physico-chemical techniques; also, effect on proliferation of cancer cells have been tested in in vitro conditions, which proved slightly cytotoxic activity. A more detailed study and an optimization of methods for in vitro characterization will be necessary to further...
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The Pedagogy of Experience and Jabok Introductory Course 2005
Šírová, Kateřina ; Ortová, Marie (advisor) ; Růžička, Vít (referee)
This final work deals with outdoor pedagogy and introduced workshop of Jabok in 2005. First there is general paper on experience and outdoor pedagogy. Then there is general description outdoor workshop (how prepore, do and appraise this workshop). The finally there is presentation of introduced workshop of Jabok in 2005 (where is mainly appraise this workshop). Powered by TCPDF (www.tcpdf.org)
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System of day care for preschool children in the Czech Republic
Šírová, Kateřina ; Poláková, Olga (advisor) ; Barák, Vladimír (referee)
Women on maternity leave are considered one of the most vulnerable groups in the labor market. Because of their prolonged failure of the working process in connection with child care they have difficult conditions in search of employment. The system of day care for preschool children should help women to eliminate this effect. The thesis is aimed to examine the adequacy and effectiveness of the systems of day care for preschool children based on the analysis of legislation and in comparison with systems of other European states. The work also compares the sufficiency of the regions of the Czech Republic and its possible effects on female unemployment.
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