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Closer analysis of gene trap line MGT180 and its candidate genes
Šnajdrová, Tereza ; Soukup, Aleš (advisor) ; Klíma, Petr (referee)
Root branching allows plants to explore rhizosphere, to gain efficiently water, mineral nutrients or enter in various biotic interactions. Initiation of lateral root formation is localized to pericycle cells, flanking the xylem poles of diarch vascular bundle in Arabidopsis thaliana. Right in these pericycle cells, there is the expression pattern of the gene trap line MGT180. In this theseis , I have provided the evidence that the expression pattern of MGT180 is related with AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED18 (AHL18; At3g60870). AHL18 belongs to a gene family of 29 transcriptional factors of Arabidopsis. AHL18 has not been functionally characterized yet, the analysis of singlemutant ahl18 and some others revealed no significant phenotype. However, one of doublemutants, E15, showed a significant phenotype. This phenotype was evident mainly in the aboveground part of plants, and was not corresponding to any phenotype of AHL mutation described so far. There is a known redundancy among some AHL genes, confirmed by crossing of ahl18 and ahl28 leading to E15 plant. Translation phusion AHL18-mRUBY and AHL22-mRUBY under native promotors should reveal, where these two related proteins act, and if they fiction in autonomous manner or not. Key words Arabidopsis thaliana, lateral root, AHL, pericycle
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Creation of GUI for optimization of electrical machines
Bártková, Tereza ; Klíma, Petr (referee) ; Knebl, Ladislav (advisor)
This work follows the development process of graphical user interface (GUI) spanning from choosing the right tools for implementation to the implementation itself. The purpose of the work was making the optimization program user-friendly by creating a communication layer between the optimization algorithm and the user and demonstrating its functions. First of all, the study of various programming languages and their toolkits regarding GUI programming was undertaken. Python was chosen because the original program is written in it, also software development in Python is probably the quickest one. Furthermore there are analyzed characteristics of this language and PyQt, which is a tool meant for GUI creation. The third chapter introduces very thoroughly the issues of electrical machines and their optimization. Introduction of these issues is followed by brief description of two optimization methods being used in electrical machines optimization. Following chapter introduces the actual machine under the optimization study, on which the final program was tested. Afterwards follows detailed description of application layout created in Qt Designer. New program structure enriched with graphical user interface is described and its functions are shown. Finally there is a guideline on how to use this program and the optimization is executed accordingly. Also, the results of the optimization are analyzed.
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Flux switching electric machine
Szabó, Hugo ; Klíma, Petr (referee) ; Ondrůšek, Čestmír (advisor)
The goal of this thesis is to create a literature search about a rotating electrical machine with switching of magnetic flux, to explain its construction concept and its operating behavior, to create an initial concept of generator, calculate chosen construction with finite element method analysis and to compare analysis results with analytical design. To create a concept of the generator one of available designing procedures will be used.
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High-speed outer rotor synchronous machine
Král, Radek ; Knebl, Ladislav (referee) ; Klíma, Petr (advisor)
The master thesis deals with the construction and design of a high-speed synchronous machine with an outer rotor. The thesis is organised into three main chapters. The first chapter deals with machine design theory as well as mechanical limits, which are significant for high speed machines. This chapter also provides examples of applications of these electrical machines. In the second chapter, the initial design of the machine is calculated analytically. This machine design is optimised and the third chapter of this work shows the results of simulations using the finite elements method, including the evaluation of these results.
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The Employer's Responsability for the Damage Caused to Employee by Work Accidents and Occupational Diseases
Klíma, Pavel ; Štangová, Věra (advisor) ; Vysokajová, Margerita (referee) ; Fischerová, Iva (referee)
v anglickém jazyce The dissertation thesis deals with a very substantial and permanent part of the labour law's field and labour law-related and the other connected relationships, i.e. the issue of the work accidents and occupational diseases, mainly from the perspective of that arised reponsability's relationship, resp. the employer's obligations to damages' compensation in relation to employee and his individual rights (claims) to the damages' compensation due to the incurred accidents in the form of a work accident or occupational disease. The author's impetus to the choise of the working accidents and occupational diseases' topic (resp. the employer's responsability for damages caused to emploees due to working accidents and occupational diseases) for the purposes of the dissertation thesis is owed to (permanently) a very substantial importance of the issue in the terms of labour law-related relationships and generally and essentially society-wide relationships, even if in despite of a very substantial importance of the issue the expert's litterature to the issue of the working accidents and occupational diseases is not to be considered as sufficient. The purpose of the dissertation thesis to analyse and evaluate the actual state of the legal regulation regarding the working accidents and...
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Skin effect in high-speed electrical machines.
Klíma, Petr ; Bárta, Jan (referee) ; Mach, Martin (advisor)
This master thesis deals with the suppression of the skin and proximity effects in high-speed machines. The first part summarizes the general knowledge of high-speed machines. The second part is devoted to the principle and possible suppression of consequences caused by skin and proximity effects. The third part shows the simulation results of models of a synchronous machine with permanent magnets. The results of these simulations reveal the consequences of skin and proximity effects. In addition, design measures are proposed to limit these and other undesirable phenomena to achieve the highest possible efficiency.
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