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Tunnelling in České Středohoří
Rychnovský, Vojtěch ; Mašín, David (advisor) ; Ježek, Jan (referee)
This work contains the information about the Prackovice tunnel, tunnel across the České středohoří highland on highway D8, geological structure of the area, advanced works and the process of construction. The work also includes a 2D numerical analysis of the Prackovice tunnel using the finite element method. Parametric study and back analysis were carried out, therefore an original model could be modified to comply with the results of geotechnical monitoring. This led to better compliance between the model and reality.
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The Role of Uncoupling in Down-regulation of Reactive Oxygen Species
Ježek, Jan ; Žáčková, Markéta (advisor) ; Modrianský, Martin (referee) ; Drahota, Zdeněk (referee)
Uncoupling attenuates Complex I-derived superoxide production by accelerating electron flux and proton pumping within Complex I. However, under circumstances leading to hampered proton pumping pathway within Complex I, e.g. due to aberrant mutations of mtDNA encoding either ND2, ND4 or ND5 Complex I H+-pumping subunit, therapeutic strategy based simply on uncoupling would fail. Experimentally, hydrophobic amiloride EIPA mimicks the model of disabled H+-pumping. We show for the first time that EIPA is a real inhibitor of H+-pumping of mitochondrial Complex I. We searched for an agent that, unlike uncoupling, would be able to counteract oxidative stress associated with obstructed proton pumping of Complex I. Mitochondria-targeted ubiquinone MitoQ10 proved to be an effective antioxidant for this purpose when the rate of superoxide formation was high due to the electron flow retardation within Complex I. Because of its pro-oxidant properties, targeted delivery of MitoQ10 as a cure to the pathological tissue is necessary. Activation of mitochondrial phospholipase iPLA2 by mild oxidative stress can provide free fatty acid hydroperoxides as the cycling substrates for UCP2 that initiates mild uncoupling leading to the attenuation of reactive oxygen species (ROS) production, as a part of feedback regulatory loop of...
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Determining the resistance of paint films due to the thickness and number of layers
Ježek, Jan ; Bomba, Jan (advisor) ; Pánek, Miloš (referee)
In the theoretical part of this work there is described the theory of coating substances: sorts of coating substances, making a coating (paint) film and requirements of coating matters. In the practical part there was being found an affect of cold liquids upon concrete coating substances, an affect of the thickness of the coating film upon the resulting surface resistence and an affect of wood upon the quality of the coating. No difference of film quality of coating substances applied to conifers and leafy wood was evidenced. The quality of coating was applied to plawood materials where pores of the upper veneer were filled with paste and so there was no difference between conifers and leafy wood. According to statistics there is no important affect upon the film quality. The coatings 80 and 150g/m2 were used, which are the limited statements of an interval recommmended by producers and the quality of coating is declared. The result: the most aggressive liquid was remover, then plum brandy, Savo liquid, vinegar and mouthwash. Jar, the preparation for washing dishes, was the least aggressive.
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Principal component analysis of Raman spectroscopy data for determination of biofilm forming bacteria and yeasts
Šiler, Martin ; Samek, Ota ; Bernatová, Silvie ; Mlynariková, K. ; Ježek, Jan ; Šerý, Mojmír ; Krzyžánek, Vladislav ; Hrubanová, Kamila ; Holá, M. ; Růžička, F. ; Zemánek, Pavel
Many microorganisms (e.g., bacteria, yeast, and algae) are known to form a multi-layered structure composed of cells and extracellular matrix on various types of surfaces. Such a formation is known as the biofilm. Special attention is now paid to bacterial biofilms that are formed on the surface of medical implants, surgical fixations, and artificial tissue/vascular\nreplacements. Cells contained within such a biofilm are well protected against antibiotics and phagocytosis and, thus, effectively resist antimicrobial attack.\nA method for in vitro identification of individual bacterial cells as well as yeast colonies is presented. Figure 1 shows an an example of the biofilm formed by Staphylococcus epidermidis bacteria and Candida parapsilosis yeasts known for forming biofilms. The\npresented method is based on analysis of spectral “Raman fingerprints” obtained from the single cell or whole colony, see figure 2(top). Here, Raman spectra might be taken from the biofilm-forming cells without the influence of an extracellular matrix or directly form the bacterial/yeast colony.
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Flow-chambers for microscopy of living cells
Čolláková, Jana ; Ježek, Jan (referee) ; Antoš, Martin (advisor)
The perfusion chamber for long term observing of live cells by the means the Coherence-Controlled Holographic Microscope (CCHM) was designed. CCHM was built and designed at the Laboratory of the optical microscopy at the Institute of Physical Engineering, Brno University of Technology. CCHM can quantitatively evaluate dynamical changes inside live cells thanks to the quantitative information about phase shift in each pixel of the image. In order to demonstrate advantages of CCHM experimentally, it is important to keep the live cells in the good conditions. This is made by adding the fresh cultivation medium for studied cells directly in the microscope. In contrast to the stationary chamber the perfusion chamber allows both the cultivation medium exchange and the application of biological reagents without the necessity of removing the chamber from the microscope. Therefore we can study the vital signs of cells before and after the application of reagents. An original perfusion system with accessories compatible with CCHM was designed. The design is based on the previously published perfusion system solutions that are referred to in this thesis. The flow characteristics and medium exchange process was discussed and a modification of the internal geometry, based on numerical simulations, was introduced. The applicability of this perfusion chamber has been proven for the CCHM and even for different types of microscopes. The reactions of tumor and epithelial cells during the change of the environment from the cultivation medium to the physiologically solution were studied.
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Parking Planning and Calculation
Ježek, Jan ; Heinrich, Jaroslav (referee) ; Radimský, Michal (advisor)
The purpose of this diploma thesis „Parking Planning and Calculation„ is car parks at selected buildings. The goal of this thesis is to optimize the selected of targeted units for calculate the number of parking spaces. First part of the work is focused on the measurement itself and data analysis. The next part of the work consists of comparison of calculated parking places according to standards with real number of parking places.
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Statistical modul for working database TIVOLE
Ježek, Jan ; Wiera, Ryszard (referee) ; MBA, Jan Beran, (advisor)
Presented thesis thesis is written for IBM Company, specifically IBM IDC Czech Republic, s.r.o. branch. The thesis describes development of statistic module for work database TIVOLE utilizing Lotus Notes program. The basic aim of the thesis is to ease creation of reports and graphical data outputs in relation to speed of operator´s work while narrowing down the required time of human resources for solving problems.
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Methodology of Dictyostelium discoideum imaging with the transmission digital holographic microscope
Křížová, Aneta ; Ježek, Jan (referee) ; Uhlířová, Hana (advisor)
The task of this bachelor’s thesis was to image Dictyostelium discoideum with a transmission digital holographic microscope and compare this imaging with a commonly used microscopy technique. This bachelor's thesis is structured into a theoretical part and an experimental part. The theoretical part describes the setting of the transmission digital holographic microscope, basic operation and physical principles of imaging with this microscope. Further the process of image reconstruction and numerical process of phase reconstruction is illustrated. At the end of this part, basic biological characteristics of observed cells of Dictyostelium discoideum are introduced. The experimental part describes the cultivation and preparation of Dictyostelium discoideum for the observations, and further the process of performed experiments. The cells of Dictyostelium discoideum have been imaged in couple experiments by a method of the classical phase contrast. By the transmission digital holographic microscope the cells of Dictyostelium discoideum have been imaged in two mediums. Images captured by the transmission digital holographic microscope were processed by a method of Dynamic phase differences and analysed.
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