National Repository of Grey Literature 66 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Childern´s Sanatorium Ostrov u Macochy
Zemánek, Pavel ; Vostrejž, Dušan (referee) ; Odvárka, Antonín (advisor)
The architectural study deals with the design of Childern´s sanatorium with speleotherapy in Ostrov u Macochy. Design works with the idea of connecting with the surrounding nature, responds to the slopping terrain and tries to fulfill a comprehensive construction program. A characteristic element is the use of natural material – wood within the curtaing walling, which defines the architectural expression of the building. Emphasis is placed on renewable energy sources.
SMV-2019-67: Vector mode
Jákl, Petr ; Zemánek, Pavel
Preparation of experimental apparatus and control software for photopolymerization of nanostructures in vector mode. The nanostructures are created by continuous movement of the sample along a given trajectory with the laser beam switched on.
Imaging via multimode optical fiber: recovery of a transmission matrix using internal references
Šiler, Martin ; Jákl, Petr ; Traegaardh, Johanna ; Ježek, Jan ; Uhlířová, Hana ; Tučková, Tereza ; Zemánek, Pavel ; Čižmár, Tomáš
Current research of life shows a great desire to study the mechanics of biological processes\ndirectly within the complexity of living organisms. However, majority of practical techniques\nused nowadays for tissue visualization can only reach depths of a few tens of micrometres as\nthe issue obscures deep imaging due to the random light scattering. Several imaging\ntechniques deal with this problems from different angels, such as optical coherence\ntomography, light sheet microscopy or structured light illumination A different and promising strategy to overcome the turbid nature of scattering tissues is to employ multimode optical fibers (MMF) as minimally invasive light guides or endoscopes to provide optical access inside. Although the theoretical description of light propagation through such fibers has been developed a long time ago it is frequently considered inadequate to describe real MMF. The inherent randomization of light propagating through MMFs is typically attributed to undetectable deviations from the ideal fiber structure. It is a commonly believed that this\nadditional chaos is unpredictable and that its influence grows with the length of the fiber.\nDespite this, light transport through MMFs remains deterministic and can be characterized by a transmission matrix (TM) which connects the intensity and phase patterns on the fiber input and output facets. Once the TM is known it can be used to create focus in any desired 3D\ncoordinates beyond the distal fiber facet, see figure 1, and perform e.g. fluorescence based\nlaser scanning microscopy or optical trapping.
Orbital motion from optical spin: the extraordinary momentum of circularly polarized light beams
Svak, Vojtěch ; Brzobohatý, Oto ; Šiler, Martin ; Jákl, Petr ; Zemánek, Pavel ; Simpson, Stephen Hugh
We provide a vivid demonstration of the mechanical effect of transverse spin momentum in an\noptical beam in free space. This component of the Poynting momentum was previously thought\nto be virtual, and unmeasurable. Here, its effect is revealed in the inertial motion of a probe\nparticle in a circularly polarized Gaussian trap, in vacuum. Transverse spin forces combine with\nthermal fluctuations to induce a striking range of non-equilibrium phenomena. With increasing\nbeam power we observe (i) growing departures from energy equipartition, (ii) the formation of\ncoherent, thermally excited orbits and, ultimately, (iii) the ejection of the particle from the trap.\nOur results complement and corroborate recent measurements of spin momentum in evanescent\nwaves, and extend them to a new geometry, in free space. In doing so, we exhibit fundamental,\ngeneric features of the mechanical interaction of circularly polarized light with matter. The work\nalso shows how observations of the under-damped motion of probe particles can provide detailed\ninformation about the nature and morphology of momentum flows in arbitrarily structured light\nfields as well as providing a test bed for elementary non-equilibrium statistical mechanics.
Optofluidic techniques for directed evolution of enzymes
Pilát, Zdeněk ; Ježek, Jan ; Samek, Ota ; Zemánek, Pavel ; Buryška, T. ; Damborský, J. ; Prokop, Z.
Enzymes are highly versatile and ubiquitous biological catalysts. They can greatly accelerate\nlarge variety of reactions, while ensuring appropriate catalytic activity and high selectivity.\nThese properties make enzymes attractive biocatalysts for a wide range of industrial and\nbiomedical applications. Over the last two decades, directed evolution of enzymes has\ntransformed the field of protein engineering.
Optical binding of polystyrene particles in tractor beam
Damková, Jana ; Chvátal, Lukáš ; Oulehla, Jindřich ; Ježek, Jan ; Brzobohatý, Oto ; Zemánek, Pavel
The motion of a particle illuminated by a laser beam is usually driven by the photon flow due\nto the radiation pressure and therefore for particle trapping, one has to employ gradient forces. But in a tractor beam, objects are illuminated by the uniform light intensity and even so they can be pulled against the beam propagation. There have been developed several techniques how to create such a tractor beam. In our case, the tractor beam is created by two identical Gaussian beams that interfere under the defined angle. It creates the\nstanding wave, where in the transversal plane the particle is trapped by means of the gradient\nforce, but in the total beam propagation direction, the particle manipulation is driven by the non-conservative force. It is remarkable that this force can for the specific combinations of\nparameters pull the micro-particle against the beam propagation. This kind of behavior is\nbecause of the particle scattering where the majority of the incident photons is scattered in the forward direction and, based on the principle of action and reaction, the transfer of\nmomentum leads to a backward movement of the object. The pushing and pulling force is\nsensitive to the polarization of the laser beam, its incident angle and the particle size so this\ntechnique can be used for example for sorting of objects of different sizes.
Multifunctional building Brno - Líšeň
Zemánek, Pavel ; Fišer, Jiří (referee) ; Odvárka, Antonín (advisor) ; Pěnčík, Jan (advisor)
Subject of this bachelor´s thesis is to develop project documents of multifunctional building in Brno – Líšeň. The building has 2 floors and 2 underground floors. On the first floor there are leasable spaces (shops). On the second floor there are leasable spaces (office, doctors). On the first underground floor there are also leasable spaces (office, doctors). On the second underground floor there is car parking. The netrance do the parking floor is secured by outdoor ramp. Structural systém of building is cast-in-place rainforced concrete. The building is based on simple fundation pads and strip foundations. An internal staircase is cast-in-place reinforced concrete. The roofs of the building are designed as warm flat roofs.
SMV-2017-24: Improving speed of photopolymerization
Jákl, Petr ; Zemánek, Pavel
Improved photopolymerization apparatus software with faster process mode.
Fluorescence and surface enhanced Raman spectroscopy in microfluidics for monitoring of enzymatic reactions
Pilát, Zdeněk ; Šmatlo, Filip ; Ježek, Jan ; Krátký, Stanislav ; Zemánek, Pavel
We have implemented two different systems for detecting the concentration of molecules in microfluidic systems. The first method uses optical fibers and detects the intensity of fluorescence, while the second method is using surface enhanced Raman spectroscopy (SERS).
Experimental fluorescence device for dielectrophoretic sorting of droplets in microfluidic chips
Ježek, Jan ; Pilát, Zdeněk ; Šmatlo, Filip ; Zemánek, Pavel
At present, many chemical and biological disciplines use different forms of spectroscopy for their observations. One of the most common methods is fluorescence spectroscopy. During the last seven years, microfluidic techniques began developing rapidly, using channels in which two immiscible liquids create droplets with diameters from units to tens to hundreds of micrometers. These droplets serve as liquid microcontainers containing the analysed sample and the necessary reagents. Using special microfluidic techniques, it is possible to fuse droplets with different contents (controlled triggering of chemical reactions), to change the concentration of reactants in the droplet with high speed (concentration gradients), or sort the droplets by content (creation of new cell strains).\n\n

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