National Repository of Grey Literature 8 records found  Search took 0.00 seconds. 
The tri-band fluorescent reader
Škrabalová, Denisa ; Čolláková, Jana (referee) ; Dostál, Zbyněk (advisor)
This bachelor thesis deals with the issue of laboratory testing and design of an optical instrument called a tri-band fluorescent reader, which is designed for fluorophores Alexa Fluor 488, Alexa Fluor 555 and Alexa Fluor 647. It contains a theoretical part with key knowledge not only in the natural sciences, such as physics, chemistry, biology, but also the current technical capabilities that are necessary for the practical part. In the practical part is drafting the objectives of the thesis, which are the measurement and evaluation of the samples examined using a monochromator, acquisition and evaluation of images using the existing system, build spectral analysis throughput, create a simulation of custom solutions and create a system engineering design. The mechanical model of the reader assembly was made after measuring the samples and the device and creating the simulation.
Fluorescence imaging techniques in multimodal holographic microscope
Vašíček, David ; Procházková, Jana (referee) ; Čolláková, Jana (advisor)
The diploma thesis deals with the registration of images taken with the multimodal holographic microscope (MHM). The summary covers the fluorescent and holographic microscopy, and the multimodal holographic microscope combining both these microscopy types. Every pair of the images needs to be aligned in order to gain new information by combining both image types. The thesis contains an algorithm that registers images by phase correlation as well as a process created in MATLAB in accordance with the algorithm. The most important procedure parameters’ influence on the registration success is described and the results are annotated.
Measurement of the distribution of an equivalent of cell dry mass using quantitative phase contrast of the CCHM
Kovářová, Klára ; Čolláková, Jana (referee) ; Křížová, Aneta (advisor)
Subject of this bachelor's thesis is to create an overview of conversions of phase profile to the values of dry mass and to realize experiments verifying the importance of dry mass. In the theoretical part, the history of the holographic microscopy and the development of the holographic microscopy on IPE FME BUT in Brno are summarized. The transmission holographic microscope is also described. This thesis covers its construction description and basic working instructions. The theoretical part also deals with the importance of image phase. Two approaches are presented for the interpretation of the phase delay and there is research on phase conversions to dry cell mass (according to H. G. Davies and M. H. F. Wilkins, G. Popescue, G. Dunn and D. Zicha). In the experimental part cultivation of cells, preparation of observation sample and processing of data are described. This part of thesis is also focused on laboratory experiments. Cells K2 (full name LW13K2) were observed in all experiments. First experiments deal with nutritional deprivation of cells and later with cell growth.
Flow-chambers for microscopy of living cells
Čolláková, Jana ; Ježek, Jan (referee) ; Antoš, Martin (advisor)
The perfusion chamber for long term observing of live cells by the means the Coherence-Controlled Holographic Microscope (CCHM) was designed. CCHM was built and designed at the Laboratory of the optical microscopy at the Institute of Physical Engineering, Brno University of Technology. CCHM can quantitatively evaluate dynamical changes inside live cells thanks to the quantitative information about phase shift in each pixel of the image. In order to demonstrate advantages of CCHM experimentally, it is important to keep the live cells in the good conditions. This is made by adding the fresh cultivation medium for studied cells directly in the microscope. In contrast to the stationary chamber the perfusion chamber allows both the cultivation medium exchange and the application of biological reagents without the necessity of removing the chamber from the microscope. Therefore we can study the vital signs of cells before and after the application of reagents. An original perfusion system with accessories compatible with CCHM was designed. The design is based on the previously published perfusion system solutions that are referred to in this thesis. The flow characteristics and medium exchange process was discussed and a modification of the internal geometry, based on numerical simulations, was introduced. The applicability of this perfusion chamber has been proven for the CCHM and even for different types of microscopes. The reactions of tumor and epithelial cells during the change of the environment from the cultivation medium to the physiologically solution were studied.
The tri-band fluorescent reader
Škrabalová, Denisa ; Čolláková, Jana (referee) ; Dostál, Zbyněk (advisor)
This bachelor thesis deals with the issue of laboratory testing and design of an optical instrument called a tri-band fluorescent reader, which is designed for fluorophores Alexa Fluor 488, Alexa Fluor 555 and Alexa Fluor 647. It contains a theoretical part with key knowledge not only in the natural sciences, such as physics, chemistry, biology, but also the current technical capabilities that are necessary for the practical part. In the practical part is drafting the objectives of the thesis, which are the measurement and evaluation of the samples examined using a monochromator, acquisition and evaluation of images using the existing system, build spectral analysis throughput, create a simulation of custom solutions and create a system engineering design. The mechanical model of the reader assembly was made after measuring the samples and the device and creating the simulation.
Fluorescence imaging techniques in multimodal holographic microscope
Vašíček, David ; Procházková, Jana (referee) ; Čolláková, Jana (advisor)
The diploma thesis deals with the registration of images taken with the multimodal holographic microscope (MHM). The summary covers the fluorescent and holographic microscopy, and the multimodal holographic microscope combining both these microscopy types. Every pair of the images needs to be aligned in order to gain new information by combining both image types. The thesis contains an algorithm that registers images by phase correlation as well as a process created in MATLAB in accordance with the algorithm. The most important procedure parameters’ influence on the registration success is described and the results are annotated.
Flow-chambers for microscopy of living cells
Čolláková, Jana ; Ježek, Jan (referee) ; Antoš, Martin (advisor)
The perfusion chamber for long term observing of live cells by the means the Coherence-Controlled Holographic Microscope (CCHM) was designed. CCHM was built and designed at the Laboratory of the optical microscopy at the Institute of Physical Engineering, Brno University of Technology. CCHM can quantitatively evaluate dynamical changes inside live cells thanks to the quantitative information about phase shift in each pixel of the image. In order to demonstrate advantages of CCHM experimentally, it is important to keep the live cells in the good conditions. This is made by adding the fresh cultivation medium for studied cells directly in the microscope. In contrast to the stationary chamber the perfusion chamber allows both the cultivation medium exchange and the application of biological reagents without the necessity of removing the chamber from the microscope. Therefore we can study the vital signs of cells before and after the application of reagents. An original perfusion system with accessories compatible with CCHM was designed. The design is based on the previously published perfusion system solutions that are referred to in this thesis. The flow characteristics and medium exchange process was discussed and a modification of the internal geometry, based on numerical simulations, was introduced. The applicability of this perfusion chamber has been proven for the CCHM and even for different types of microscopes. The reactions of tumor and epithelial cells during the change of the environment from the cultivation medium to the physiologically solution were studied.
Measurement of the distribution of an equivalent of cell dry mass using quantitative phase contrast of the CCHM
Kovářová, Klára ; Čolláková, Jana (referee) ; Křížová, Aneta (advisor)
Subject of this bachelor's thesis is to create an overview of conversions of phase profile to the values of dry mass and to realize experiments verifying the importance of dry mass. In the theoretical part, the history of the holographic microscopy and the development of the holographic microscopy on IPE FME BUT in Brno are summarized. The transmission holographic microscope is also described. This thesis covers its construction description and basic working instructions. The theoretical part also deals with the importance of image phase. Two approaches are presented for the interpretation of the phase delay and there is research on phase conversions to dry cell mass (according to H. G. Davies and M. H. F. Wilkins, G. Popescue, G. Dunn and D. Zicha). In the experimental part cultivation of cells, preparation of observation sample and processing of data are described. This part of thesis is also focused on laboratory experiments. Cells K2 (full name LW13K2) were observed in all experiments. First experiments deal with nutritional deprivation of cells and later with cell growth.

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