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Konstrukční řešení tenzometrické měřicí hlavy
Kukla, Jan
The main objective of my thesis is to design the constuction of strain gauge mea-surring head. This constrction allows testing float in the pool and facilitate the job of a human operator. By measuring we determined the physical properties of the float. The construction is mounted on the robot KUKA. I chose suitable sensor single-point for measuring buoyacy and tensile strength float on the surface. Strain sensor has an cover IP67. Material of the supportng structure is made of aluminium alloy from company MayTec. The sensors are located in the axis of the center of gravity of the float, which i experimentally calculated. From my experience, character of the float in work on the surface and under load be expected change center of gravity. Construction is adapted for this situation. Final product is light and durable construction with precise sensors and low price.
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Preparation and use of acid proteases for digestion in H/D exchange.
Kukla, Jan ; Man, Petr (advisor) ; Pompach, Petr (referee)
- 5 - Abstract Hydrogen/deuterium exchange coupled to mass spectrometry (HX-MS) utilizes the spontaneous exchange of protein backbone amide hydrogens for deuterium atoms from solution to gain information about changes in protein structure. To localize these changes to specific areas of the protein, enzymatic digestion by aspartate proteases is used. The proteases' ability to produce small overlapping peptides and to provide full sequence coverage of the studied protein is essential for pinpointing the protein regions of interest. In this study recombinant proteases nepenthesin I (Nepenthes gracilis) and rhizopuspepsin (Rhizopus chinensis) were prepared and compared to commercially available proteases porcine pepsin A and aspergillopepsin (Aspergillus saitoi). The comparison was performed using various activity assays, where the effects of pH, temperature and denaturing and reducing agents on the activity of the proteases were studied. All four proteases were also immobilized on a polymeric resin POROS and their activity in an online HX-MS digestion setup was tested using myoglobin as a model substrate.
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Assessing the impact of attendance on the selected caves using GIS
Kukla, Jaroslav ; Matějíček, Luboš (advisor) ; Frouz, Jan (referee)
Caves are valuable sites from geological , palaeontological and biological point of view. They are important biotopes of rare and endangered species. Their ecosystem is quite specific and often very independent on environment. Therefore, the caves since the beginning of science have been the subject of research , and gradually also the subject of protection. Caves can also be very attractive objects of interest of tourism. In the Czech Republic there are 14 open to the public karst caves that have regulated attendance, and their access time is subordinated to the interests of nature conservation. On the other hand, there are also a number of caves that are not secured in any way and may be a target of unregulated tourism. This dissertation discusses the evaluation and the impact of attendance on non-karst caves in the National Nature Reserve Canyon Labe, which are freely accessible. I divided the work into three parts. In the first part I dealt with quantifying the attendance of all caves in the area of interest, On the basis of collected data, I made a selection of caves , which I later focused on. In the second part of my work I focused on obtaining information about the visitors and their motivations using public inquiry survey in selected caves, In the third part I tried to analyze the impact...
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Functional analysis of plant Arp2/3 complex subunits
Kukla, Jakub ; Schwarzerová, Kateřina (advisor) ; Honys, David (referee)
1. Abstract ARP2/3 complex is well studied in case of animals, it plays key roles in motility of cells and intracellular organels. It's malfunctions result in severe growth disorders and even lethality of affected cells. On the contrary, plant cells do not exhibit such dramatic phenotype of ARP2/3 complex mutations like it is by animals. It is possible that just the different life strategies of plants and animals contribute to differences in a way how animal and plant cells use their cytoskeleton, where ARP2/3 complex is it's part as well. It is highly conserved 7 protein complex from yeast to human. His main functions are creation of new "de novo" actin filaments, actin branched filaments network. Some of the parasite organisms are capable of missusing its nucleator activity to actively move inside of host cell. Because of the plant cells are sourounded by the cell wall, which give them support in creating various shapes and also hinders active movement of the whole cell body, it is likely that ARP 2/3 complex could be possibly involved in novel plant specific functions as well. If we think about the different life strategy of plants and animals we can not ignore all the things these two kingdoms have in common regarding to cytoskeleton processes. That is the need both for vesicular transport and...
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Safety problems of modern nuclear reactors
Kukla, Jaroslav ; Matějíček, Luboš (referee) ; Přibil, Rudolf (advisor)
Jaroslav Kukla Otázky bezpe nosti moderních jaderných reaktor Bakalá ská práce Abstract: This study is concerned with safety factors of nuclear reactors. It summarizes history of nuclear reactors development and analyses causes of some serious accidents, which occurred in the past. It describes physical characteristics of radioactive radiation and mentions a basic survey of quantities and units. Then it describes the influence of radioactive emission on man and other living organisms. It also defines some reactor components and focuses on general security principles, which are further developed. It shortly explains the methods of evaluation of safety factors of nuclear reactors and a role of nuclear supervision. It puts nuclear reactors into several generations and every type of reactor is described as for technical and safety aspects, respectively. It refers to contemporary trends in development of nuclear reactors and in development of their safety.
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Final report about research in Sokolov post mining sites in 2016 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Vindušková, O. ; Pikl, M. ; Angst, Šárka ; Kukla, J. ; Moradi, J. ; Vicentini, Fabio ; Buchbauerová, L. ; Tesnerová, C. ; Jandová, K. ; Kučera, J. ; Mudrák, O.
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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Mystery Shopping
Kukla, Jiří ; Pilař, Ladislav (advisor) ; Balcarová, Tereza (referee)
This thesis is about research method mystery shopping, which is one of quantitative research methods. The functionality of the method was verified in practice - store evaluation, especially employee evaluation in three selected stores of the producer and seller of luxury clothing. The thesis is divided into two parts - theoretical and practical.
The first part consists of a literature review with used literature and internet sources.
The second part, a practical part is focused on a complete mystery shopping project creation beginning with preparation until its final part - the results evaluation and comparison. For the purpose of the project was necessary to create a scenario for mystery shopping check and also a survey. This pilot project was focused on evaluation of customer service in three selected stores - two in Prague, one of them in the city centre, and one shop in Brno. The results were then evaluated for each store separately and the results were compared between individual stores themselves. The overall results were used to make some recommendations primarily to improve customer service.
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Possibilities of Using the Six Sigma Method in a Company
Kukla, Jakub ; Fejfarová, Martina (advisor) ; Huml, Jan (referee)
Nowadays, business philosophy or methodology called Six Sigma is considered to be one of the leading tools of quality management. This bachelor thesis is focused on the description and practical demonstration of this methodology. Theoretical part of this thesis describes three different approaches to this methodology which can be understood as a tool of defects measurement, management system or methodology of continual improvement. The essential factor of Six Sigma philosophy is teamwork. This bachelor thesis concisely describes the main roles of the team members and their responsibilities. The key element of this thesis is the description of DMAIC model and its phases, outputs and tools used. Besides this, basic information about DMADV model which is used to design new processes can be found here. The practical part of this thesis describes the implementation of e-shop conversion rate increase project in the company Delmas s.r.o. The data on which the project is based are collected through quantitative and qualitative analysis, interview and company documentation analysis. Using the model DMAIC the data are transformed into the solution which is tested and implemented afterwards. The final part of this thesis summarizes the results of this project, its economical impact and future recommendations.
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