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Návrh marketingové strategie pro uvedení těstovin s hmyzím proteinem na trh
Šulc, Marek
Šulc, M. Developing a marketing strategy of launching a pasta with insect protein. Bachelor thesis. Brno: Mendel University, 2023. This bachelor's thesis focuses mainly on the specifics of marketing strategies for products containing insect protein. The first part of the thesis deals with literature related to the investigated issue. In the second part, a survey of the Czech market is carried out and then a marketing strategy is proposed, including the quantification of costs, for the entry of a new product containing insect protein into the market.
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Body surfaces of reptiles as projection screens of evolution
Abramjan, Andran ; Frynta, Daniel (advisor) ; Rehák, Ivan (referee) ; Šulc, Michal (referee)
The PhD thesis deals mainly with the coloration (and also scalation) of selected reptile species and explores evolutionary or ecological contexts indicated by these traits. The main topics can be roughly divided into two groups: 'antipredation signals' and 'effects of parthenogenesis'. The dominant method used in most studies is visual modelling. The work consists of the following case studies. 1) Blue-tongue skinks use their conspicuous blue tongues to threaten potential predators. We found that the tongue has a relatively high UV reflectance, a typical feature of intraspecific communication in lizards. Using visual models, we investigated how the blue tongue is perceived by the conspecifics and predators (birds of prey). In both visual models, the UV-blue tongue appears more conspicuous against the natural background than a pink tongue. In addition, in the conspecifics model, its hue partially overlaps with hues of UV-blue spots, which are sexually selected traits in various species of lizards. Thus, the UV-blue tongue seems to contribute to the effectiveness of the deimatic display and its possible role in intraspecific communication cannot be ruled out either. 2) We detected UV reflectance in non-pigmented areas of the skin in the Leopard gecko (Eublepharis macularius). These form white patches...
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Structure and interaction of human 14-3-3 regulatory protein using in vitro photoaffinity labelling in combination of protein nano-probes and mass spectrometry
Mazurová, Martina ; Šulc, Miroslav (advisor) ; Dračínská, Helena (referee)
This thesis is focused on the study of the structure and mechanism of human 14- 3-3 protein, which is one of the important regulatory proteins present in all eukaryotic cells. Nowadays it is known seven isoforms of this protein in mammals. Although their crystal structure shows a high similarity, their mutual comparison reveals some changes. The aim of this work is to prepare experimental tools for verification whether the differences in the crystal structure of the ζ isoform are present in solution and how the structure-functional mechanism of this isoform is affected. The otimization of 14-3- 3zeta recombinant protein expression with incorporated a photo-labile analog of leucine in the protein sequence was performed using limiting medium with prokaryotic expression system of E. coli BL-21 DE3 Gold or system of auxotrophic E. coli K-12 with non-functional leucine biosynthesis.
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Impact evaluation of foreign direct investment on the financial situation of an internationally operating enterprise and suggestions for its improvement
Šulc, Michal ; Hornungová, Jana (referee) ; Procházková, Kateřina (advisor)
This thesis is focused on the evaluation of the impact of foreign direct investment on the financial situation of the internationally operating company ČESKÁ LÉKÁRNA HOLDING, a.s. The theoretical part includes a comprehensive analysis of foreign direct investment from various aspects, financial analysis, selected indicators, analysis of external and internal environment and methodological approaches including statistical tests for subsequent research. The analytical part includes selected indicators for the period 2000 to 2021 presented in graphical form as well as research aimed at demonstrating the impact of foreign direct investment. The design section proposes solutions that are expected to induce positive or economically beneficial changes with the anticipated result of improving the company's financial situation.
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Functional analysis of insect fatty acyl-coenzyme A reductases and desaturases
Tupec, Michal ; Pichová, Iva (advisor) ; Šulc, Miroslav (referee) ; Doležel, David (referee)
Fatty acid-derived lipids are an important class of biomolecules. In addition to their primary role in cellular architecture, energy storage and signaling, they function in many other ways, e.g. as isolating or waterproof coatings, defense compounds and pheromones. Multiple enzymes mod- ify intermediates originating from fatty acid biosynthetic pathway, including desaturases (FADs) which synthesize unsaturated fatty acids, and reductases (FARs) which synthesize fatty alcohols. Functionally highly diversified fatty acyl-modifying enzymes from insects, one of the most abun- dant animals in the world, present a significant source for modern biotechnology of cell factories. This thesis summarizes available information on the FADs and FARs and describes the results which we have obtained while studying them. In bumblebees, we identified several FAR transcripts which are abundantly expressed in male pheromone-producing tissue. We then functionally characterized the corresponding enzymes in yeasts, and estimated their participation in the biosynthesis of fatty alcohols observed in the mark- ing pheromone-producing tissue. The studied enzymes reduce broad range of substrates, from short fatty acyls (e.g. C14) to very long ones (e.g. C26), from saturated fatty acyls to polyunsatu- rated ones. We also found...
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The Virgin Mary in the Work of Bohuslav Reynek
Šulc, Martin ; Šmejdová, Barbora (advisor) ; Vopřada, David (referee)
The thesis deals with the research of pictorial and poetic work, the motif of which is the Virgin Mary, in the work of the poet and painter Bohuslav Reynek. The aim of the work is a deeper knowledge of the topic and penetration into the Marian theme in the work of Bohuslav Reynek, based on Roman Catholic Christian pre-understanding, especially in the field of systematic theology - mariology. The work is conducted with regard to the literary and pictorial meaning of the subject. The main tool used is the available literature dealing with the issue.
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Structure-function relationship of Kingella kingae RtxA toxin.
Růžičková, Eliška ; Osička, Radim (advisor) ; Šulc, Miroslav (referee)
Kingella kingae is a pediatrically significant, facultative pathogen. It asymptomatically colonizes the oropharynx of young children, where it is a part of the normal microflora. However, if it penetrates the respiratory epithelial barrier and begins to spread throughout the body, it can cause serious infectious diseases. Thanks to today's advanced diagnostic methods, K. kingae is included among important human pathogens, and in pediatric patients, K. kingae is reported as a frequent cause of osteoarticular infections, such as osteomyelitis and septic arthritis, bacteremia, and endocarditis. The key virulence factor of this bacterium, the cytotoxin RtxA, belongs to the RTX (Repeats in ToXin) toxin family. This family of toxins shares several characteristic features: (i) the presence of a hydrophobic pore-forming domain in the N-terminal part of the molecule containing several predicted transmembrane α-helices (ii) the inactive protoxin is activated by different types of fatty acids bound to specific lysine residues in the acylated domain, (iii) the presence of nonapeptide repeat sequences, rich in glycine and aspartate residues, that are important for the binding of calcium ions, (iv) the presence of a C-terminal secretion signal that is recognized by the type I secretion system (T1SS), and (v)...
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Structure and function of RTX toxins of Gram-negative bacteria
Zhuk, Karyna ; Osička, Radim (advisor) ; Šulc, Miroslav (referee)
RTX toxins (Repeats in ToXin) are produced by Gram-negative bacteria, most of which are important human or animal pathogens. The polypeptide chain of each RTX toxin consists of four conserved regions. An N-terminal hydrophobic domain, which is important for insertion of the RTX toxin into the host cell membrane and pore formation. The hydrophobic domain is followed by an acylated segment containing conserved lysine residues, at which the toxin is acylated and thus activated. The C-terminal portion of each RTX toxin contains a repeat domain to which calcium ions bind. The C-terminus of the toxin contains a secretion signal that is recognized by the type I secretion system, which transports the toxin from the bacterial cytosol to the external environment. After secretion, RTX toxins interact with the cell surface via specific β2 integrins and/or glycosylated structures such as glycoproteins and gangliosides or membrane components such as sphingomyelins and cholesterol. Once bound to the cell, RTX toxin monomers insert into the membrane and oligomerize to form pores. The uncontrolled flow of ions through these pores can lead to disruption of bactericidal functions of myeloid phagocytes, stimulation or suppression of the release of pro-inflammatory cytokines, modulation of various signaling and...
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Proteomická a bioinformatická charakterizace N-terminálních sekvencí proteinů modifikovaných po importu do hydrogenosomu Trichomonas vaginalis.
Zákoucká, Eva ; Man, Petr (advisor) ; Šulc, Miroslav (referee)
Trichomonas vaginalis is a human pathogen causing trichomoniasis, one of the most common non-viral sexually transmitted diseases in both men and women. Trichomoniasis is currently treated with metronidazole, but the pathogen is known to develop resistance against this drug. However as the pathogen is eukaryotic, the targets for the pathogen elimination without seriously affecting the host are limited. Throughout the evolution Trichomonas vaginalis adapted to anaerobic environments by developing an alternative metabolism resulting in a reduced form of mitochondria named hydrogenosome. Hydrogenosomes lack genetic information, therefore all its proteins are nucleus-encoded and need to be transported inside the hydrogenosome using a targeting N-terminal presequence. The peptidase recognizing and cleaving those presequences at the entrance of the organelle, the hydrogenosomal processing peptidase (HPP), is unique for hydrogenosomes and therefore represents a potential drug target against the pathogen. In this work the HPP's substrate specificity towards the targeting presequences was investigated. To do so a proteomic analysis of the proteome of Trichomonas vaginalis hydrogenosomes was performed using a novel optimized protocol for N-terminal peptide sequencing. N-terminal peptides were captured using a...
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