|
|
Immunohistochemical analysis of the effect of M1043 on endoglin expression in a mouse model of NASH
Kubíčková, Adéla ; Němečková, Ivana (advisor) ; Kudláčková, Zděnka (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Biological and Medical Sciences Candidate: Adéla Kubíčková Supervisor of Bachelor thesis: RNDr. Ivana Němečková, Ph.D. Title of Bachelor thesis: Immunohistochemical analysis of the effect of M1043 on endoglin expression in a mouse model of NASH The morphological and functional changes of the endothelial cells of the hepatic sinusoids and endothelial dysfunction occur during nonalcoholic steatohepatitis (NASH). It has been shown that the expression of adhesion molecules (VCAM-1, ICAM-1) and endoglin increase in NASH. Endoglin affects the activation of hepatic stellate cells, which attenuate the process of fibrotization. The aim of this bachelor thesis was to detect the expression of endoglin and the adhesion molecule VCAM-1 in a mouse model of NASH after 8 weeks of CDAA diet (choline-deficient and L-amino acid-defined diet). Mice were divided into 2 groups (n=8). The control group was injected with rat IgG antibody (CDAA+rat IgG group) for 4 weeks and the CDAA+M1043 group had anti-endoglin antibody M1043. The analysis of selected proteins expression was performed by immunohistochemical method using avidin-biotin method. The results of this thesis show that there was not any reduction in the expression of VCAM- 1 or...
|
|
|
Comparison conductive detectors for capillary electrophoresis
Kříček, Zdeněk ; Křížek, Tomáš (advisor) ; Kubíčková, Anna (referee)
The aim of this bachelor thesis is comparison of two contactless conductivity detectors in capillary electrophoresis. Two methods were used for comparison: The determination of saccharides, namely glucose, fructose and sucrose, and then the determination of inorganic ions, such as chlorides, nitrates, and sulphates. The theoretical part deals with capillary electrophoresis in general and then the specific use of a contactless conductivity detector in capillary electrophoresis and its optimization. Parameters used for comparison of detectors such as limit of detection or relative standard deviation are described. Afterwards, different methods that can be used for the separation and determination of saccharides and inorganic ions are reviewed. The experimental part contains data that were measured in the optimization part of this study as well as comparison of the two detectors used, Admet and TraceDec. The comparison part starts with the method for the separation of saccharides using short-end injection mode in 40mmol∙l−1 sodium hydroxide with a separation voltage of −15 kV. The internal standard was 0,1mg∙ml−1 lactose. The calibration curve was measured in the concentration range from 0,01 to 0,5 mg∙ml−1 The linearity was measured with values of LOD, LLOQ and RSD determined as well. The values of...
|
|
|
Utilization of ultra-high performance liquid chromatography for simultaneous determination of paracetamol, amoxicillin, and doxycycline
Hrašková, Hana ; Kubíčková, Anna (advisor) ; Sobotníková, Jana (referee)
In this study, a method was developed for the simultaneous determination of three pharmaceutical compounds: amoxicillin, doxycycline, and paracetamol. Ultra-high performance liquid chromatography (UHPLC) was employed for the separation. A Kinetex column C18 measuring 100×2,1 mm with a particle size of 1,7 μm was used as the stationary phase. The optimal method was run in gradient mode with a total analysis time of 5 minutes. The mobile phase consisted of a mixture of component A, containing acetonitrile with 0,1% formic acid, and component B, comprising deionized water with 0,1% formic acid. The separation conditions in the column varied according to the following gradient program: 0 - 1,0 min: 10% A (v/v); 1,0 - 1,5 min: 10% → 65% A (v/v); 1,5 - 2,0 min: 65% A (v/v); 2,0 - 2,5 min 65%→10% A (v/v); 2,5 - 5,0 min 10% A (v/v). The pH of the mobile phase was maintained at 2,8. The flow rate was set at 0,3 ml/min, column temperature was maintained at 25řC, and the autosampler temperature was consistently held at 8řC. The injection volume was 1 μl, and analytes were detected using a diode array detector at a wavelength of 245 nm. Drug identification was conducted using a QDA detector set to positive mode with a mass range from 100,00 to 500,00 Da. The method was partially validated by constructing a...
|
|
|
Verification of the authenticity of remains of 18th century medicinal products containing juniper or liquorice
Lener, Tomáš ; Nesměrák, Karel (advisor) ; Kubíčková, Anna (referee)
Three remains of historical pharmaceuticals from 18th century containing juniper (Rob juniperi, Lignum juniperi) or liquorice (Pulvis radicis liquiritiae) were analyzed by HPLC-MS method and their authenticity was verified using a chemotaxonomic approach. Current reference material was the source of chemotaxonomic markers and also a raw material for replication of Rob juniperi according to two period recepies. HPLC-MS method provided good results but wasinsufficienttoanalyzeLignumjuniperi.Therefore,GC-MSmethod wasalsousedto analyzethejunipersamplesand it provided sufficient results to prove the authenticity of both juniper samples. HPLC-MS method identified viridiflorin heptoside for the first time in juniper material, for which as well as for five liquorice markers ESI fragmentation spectra weremeasured and theirpossiblefragmentation mechanismwasproposed.Based on theobtained results, thepreparation ofhistorical samplesfrom the mentioned plants can beconfirmed. Key words: HPLC-MS, GC-MS,Chemotaxonomy, Juniper,Liquorice, Tandemmass spectrometry,Viridiflorin
|
|
|
Characterization and application of zwitterionic stationary phases in HPLC
Machová, Dominika ; Kalíková, Květa (advisor) ; Kubíčková, Anna (referee)
This diploma thesis deals with the characterization and comparison of two zwitterionic stationary phases of SeQuant ZIC-HILIC and Atlantis Premier BEH Z-HILIC columns. Analyses were performed in the hydrophilic interaction liquid chromatography (HILIC) separation mode. The ligand bound to both stationary phases of these columns is identical, sulfoalkylbetaine. The main difference between these stationary phases is in the particles to which the ligands are bound. The type of the particles significantly contributes to different interaction and separation properties of the stationary phases. Firstly, for the basic characterization of these stationary phases, a Tanaka's test was performed. The results of this test showed that the SeQuant ZIC-HILIC column has greater cation-exchange properties than the Atlantis Premier BEH Z-HILIC column, which, has on the other hand greater anion-exchange properties. Both stationary phases showed a higher value of the parameter describing hydrophilicity than hydrophobicity, confirming their polar nature. The properties of the stationary phases were further examined and compared based on the analysis of model analytes. These analytes included a range of small polar analytes, acids, bases, ampholytes, neutral analytes, and their mixtures. Analyses were performed at...
|
|
|
Climate of a school class at a primary school
Kubíčková, Anna ; Hejlová, Helena (advisor) ; Nekardová, Barbora (referee)
This diploma thesis focuses on the social climate in primary school classrooms. It aims to assess whether classroom teachers have an overview of the social climate in their classes. The thesis is divided into theoretical and practical part. The theoretical part examines the social climate of the classroom and the school. Additionally, it deals with aspects of inclusion and integration in the educational environment, as well as classroom management strategies that influence the classroom social climate. In the practical part, teachers' characteristics of the climate in their class are compared with the results of climate examinations conducted in their classes. The research happened in three classrooms and combined qualitative and quantitative approach. Three instruments were used for data collection: a questionnaire, interviews, and a observation. During the research evaluation phase, the averaged student responses in the questionnaire were compared with the teachers' responses, and data triangulation was performed. The completed questionnaires were compared not only with each other, but with the teachers' characteristic of the classroom climate obtained through interviews and observations as well. The research findings indicate that the teachers' perceptions of the social climate in their...
|
|
| |
|
|
Preprocessing of R-R interval tachogram
Had, Filip ; Kozumplík, Jiří (referee) ; Kubičková, Alena (advisor)
Heart rate variability (HRV) is a parameter, which monitors variability of the time intervals between consecutive heart beats. Signal HRV contains artefacts and trends, which is necessary to remove before further processing. This work contains a brief information about the HRV and ECG signal processing. Further on the most frequently methods of heart rate variability preprocessing are described. The work includes a brief assessment of each method. Further on, several of the mentioned preprocessing algorithms are realized and described in this work. The last part is testing an evaluating of programmed algorithms on generated data and then demonstration on real data.
|
|
|
Sleep stage classification based on HRV signals
Schlorová, Hana ; Kubičková, Alena (referee) ; Kozumplík, Jiří (advisor)
The most common method for scoring of sleep stages is the evaluation by EEG. This work utilizes ECG signal to the comparable evaluation of sleep. It summarizes the methods of presentation and assessment of heart rate variability (HRV) and describes the whole algorithm of calculation and presentation of this signal using Lorenz plot. This work also focuses on evaluation of Lorentz plots and parametrs quantifying variability of samples in maps. It seeks to draw the conclusion of sleep stages from their waveform.
|
|
|
Documentation of Skalní zámek passage in Býčí skála cave - southern part
Kubíčková, Alena ; Hanzl, Vlastimil (referee) ; Kratochvíl, Radim (advisor)
This bachelor thesis deals with speleological mapping of a part of the cave system Býčí skála in the Moravian Karst. The part that has been mapped is called Skalní zámek. For the purpose of this work documentation was processed for the southern part of the Skalní zámek. The existing graphic materials of the interest site were searched for and described. For processing purposes, available numerical data were also found, which were compared with the new measurement during processing. The polygon of Skalní zámek was measured by mining suspension compasses. For detailed measurements, a combination of the modified DistoX2 - laser distance range-finder and TopoDroid - software application per the appropriate tablet, was used. The results of the measurements were processed in an a free and open-source cave surveying software - Therion. In this program a ground plan of the Skalní zámek, a longitudinal section of the main hallway and a longitudinal section of the branching hallway was prepared. At the end of this work the symbol lists used by TopoDroid and Therion were compared with the official UIS symbol list
|
guest ::
RSS feed.