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Verification of the authenticity of remains of 18th century medicinal products containing juniper or liquorice
Lener, Tomáš ; Nesměrák, Karel (advisor) ; Kubíčková, Anna (referee)
Three remains of historical pharmaceuticals from 18th century containing juniper (Rob juniperi, Lignum juniperi) or liquorice (Pulvis radicis liquiritiae) were analyzed by HPLC-MS method and their authenticity was verified using a chemotaxonomic approach. Current reference material was the source of chemotaxonomic markers and also a raw material for replication of Rob juniperi according to two period recepies. HPLC-MS method provided good results but wasinsufficienttoanalyzeLignumjuniperi.Therefore,GC-MSmethod wasalsousedto analyzethejunipersamplesand it provided sufficient results to prove the authenticity of both juniper samples. HPLC-MS method identified viridiflorin heptoside for the first time in juniper material, for which as well as for five liquorice markers ESI fragmentation spectra weremeasured and theirpossiblefragmentation mechanismwasproposed.Based on theobtained results, thepreparation ofhistorical samplesfrom the mentioned plants can beconfirmed. Key words: HPLC-MS, GC-MS,Chemotaxonomy, Juniper,Liquorice, Tandemmass spectrometry,Viridiflorin
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Characterization and application of zwitterionic stationary phases in HPLC
Machová, Dominika ; Kalíková, Květa (advisor) ; Kubíčková, Anna (referee)
This diploma thesis deals with the characterization and comparison of two zwitterionic stationary phases of SeQuant ZIC-HILIC and Atlantis Premier BEH Z-HILIC columns. Analyses were performed in the hydrophilic interaction liquid chromatography (HILIC) separation mode. The ligand bound to both stationary phases of these columns is identical, sulfoalkylbetaine. The main difference between these stationary phases is in the particles to which the ligands are bound. The type of the particles significantly contributes to different interaction and separation properties of the stationary phases. Firstly, for the basic characterization of these stationary phases, a Tanaka's test was performed. The results of this test showed that the SeQuant ZIC-HILIC column has greater cation-exchange properties than the Atlantis Premier BEH Z-HILIC column, which, has on the other hand greater anion-exchange properties. Both stationary phases showed a higher value of the parameter describing hydrophilicity than hydrophobicity, confirming their polar nature. The properties of the stationary phases were further examined and compared based on the analysis of model analytes. These analytes included a range of small polar analytes, acids, bases, ampholytes, neutral analytes, and their mixtures. Analyses were performed at...
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Climate of a school class at a primary school
Kubíčková, Anna ; Hejlová, Helena (advisor) ; Nekardová, Barbora (referee)
This diploma thesis focuses on the social climate in primary school classrooms. It aims to assess whether classroom teachers have an overview of the social climate in their classes. The thesis is divided into theoretical and practical part. The theoretical part examines the social climate of the classroom and the school. Additionally, it deals with aspects of inclusion and integration in the educational environment, as well as classroom management strategies that influence the classroom social climate. In the practical part, teachers' characteristics of the climate in their class are compared with the results of climate examinations conducted in their classes. The research happened in three classrooms and combined qualitative and quantitative approach. Three instruments were used for data collection: a questionnaire, interviews, and a observation. During the research evaluation phase, the averaged student responses in the questionnaire were compared with the teachers' responses, and data triangulation was performed. The completed questionnaires were compared not only with each other, but with the teachers' characteristic of the classroom climate obtained through interviews and observations as well. The research findings indicate that the teachers' perceptions of the social climate in their...
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Preprocessing of R-R interval tachogram
Had, Filip ; Kozumplík, Jiří (referee) ; Kubičková, Alena (advisor)
Heart rate variability (HRV) is a parameter, which monitors variability of the time intervals between consecutive heart beats. Signal HRV contains artefacts and trends, which is necessary to remove before further processing. This work contains a brief information about the HRV and ECG signal processing. Further on the most frequently methods of heart rate variability preprocessing are described. The work includes a brief assessment of each method. Further on, several of the mentioned preprocessing algorithms are realized and described in this work. The last part is testing an evaluating of programmed algorithms on generated data and then demonstration on real data.
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Sleep stage classification based on HRV signals
Schlorová, Hana ; Kubičková, Alena (referee) ; Kozumplík, Jiří (advisor)
The most common method for scoring of sleep stages is the evaluation by EEG. This work utilizes ECG signal to the comparable evaluation of sleep. It summarizes the methods of presentation and assessment of heart rate variability (HRV) and describes the whole algorithm of calculation and presentation of this signal using Lorenz plot. This work also focuses on evaluation of Lorentz plots and parametrs quantifying variability of samples in maps. It seeks to draw the conclusion of sleep stages from their waveform.
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Documentation of Skalní zámek passage in Býčí skála cave - southern part
Kubíčková, Alena ; Hanzl, Vlastimil (referee) ; Kratochvíl, Radim (advisor)
This bachelor thesis deals with speleological mapping of a part of the cave system Býčí skála in the Moravian Karst. The part that has been mapped is called Skalní zámek. For the purpose of this work documentation was processed for the southern part of the Skalní zámek. The existing graphic materials of the interest site were searched for and described. For processing purposes, available numerical data were also found, which were compared with the new measurement during processing. The polygon of Skalní zámek was measured by mining suspension compasses. For detailed measurements, a combination of the modified DistoX2 - laser distance range-finder and TopoDroid - software application per the appropriate tablet, was used. The results of the measurements were processed in an a free and open-source cave surveying software - Therion. In this program a ground plan of the Skalní zámek, a longitudinal section of the main hallway and a longitudinal section of the branching hallway was prepared. At the end of this work the symbol lists used by TopoDroid and Therion were compared with the official UIS symbol list
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Modeling of RR intervals
Jach, Daniel ; Kozumplík, Jiří (referee) ; Kubičková, Alena (advisor)
This thesis is focused on modelling RR intervals. Different models are created illustrating the function of cardiovascular system in order to test various methods for expressing, removing trend and analyzing heart rate variability signals. In this thesis the IPFM model as well as the McSharry model are described and realized.
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Suppression of artifacts in electrocardiograms
Šafárová, Marcela ; Kubičková, Alena (referee) ; Janoušek, Oto (advisor)
The bachelor’s thesis on the subject removal of ECG noise introduces processing of ECG signal for possible better diagnostics. The first part of the work is focused on initiation and propagation of action potential and methods for measuring heart´s electrical activity. Further are referred main types of noise affecting the ECG signal and basic filtering methods. The work focuses on filtration using Lynn´s filters, IIR filters, interpolation, spectral lines editing and adaptive filters and evaluation of their effectivity.
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Nonlinear methods of heart rate variability analysis in metabolic syndrome
Vaňková, Eliška ; Kozumplík, Jiří (referee) ; Kubičková, Alena (advisor)
This work deals with the problematic of heart rate variability of patients with metabolic syndrome. Includes the theory, which describes the heart rate variability and the methods for its analysing. Especially it specifies nonlinear methods, particularly Lyapunov exponent and its calculation in Matlab software. Algorithm of the calculation is applicated to already measured tachogram of the healthy people and patients with the metabolic syndrome. Further, this work deals with general valorization of the results using Lyapunov exponent and her eis also done the statistical evaluation.
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