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Comparison of relationships beetwen founders and headmasters of primary schools in the Czech Republic and Slovak Republic
Mrzílek, Ondřej
The objective of the thesis is an analysis, description and subsequent comparison of different aspects in relationships between the founders and the headmasters of primary schools, and subsequently a presentation of possible differences and similarities in these relationships in the Czech Republic and in Slovakia, in both legal and non-legal framework; and a description of the real situation of these relationships more than 20 years after the dissolution of Czechoslovakia. The starting point for the research are relevant legal regulations, such as acts of parliament, delegated legislation and specific legal and other documents concerning founders of private and denominational schools. The Czech and Slovak educational systems had the same development since the foundation of Czechoslovakia in 1918. After the dissolution of Czechoslovak federation the development and changes in Czech and Slovak educational systems had a different, separate development. The relationship between the school founder and its headmaster has significant influence on the school activities and direction. The thesis describes similarities and differences in these relationships in both countries and points out possibilities for mutual inspirations.
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Expression of cholinergic gene locus in a mouse model of Alzheimer's disease
Zimčík, Pavel
(anglický jazyk) The most common senile dementia, Alzheimer disease (AD), is characterized by a decline of memory and high cognitive functions. Typical post-mortem brain lesions are extracellular amyloid deposits, intracellular neurofibrilary tangles and ruined cholinergic and other neurotransmitters systems. Connection between damaged central cholinergic system and beta-amyloid accumulation remains obscure. We examined parietal cortex of young adult (7- month-old) female APPswe/PS1dE9 double transgenic mice which develope beta-amyloid fragments at high rate. Cholinergic synapses of these mice demonstrate functional presynaptic (stimulated acetylcholine release) as well as postsynaptic (muscarinic receptor-induced Gprotein activation) deficits and reduction of cholinergic markers. The mRNA levels of choline acetyltransferase, vesicular acetylcholine transporter and M1 to M4 subtypes of muscarinic receptors were determined in transgenic and littermate controls using qPCR. Obtained experimental data does not show any changes in measured mRNA levels. These observations indicate that reduction of cholinergic synaptic markers and function is due to posttranscriptional events.
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The impact of the cartographic method on students' map skills level
Havelková, Lenka
The presented diploma thesis focuses on the map skills' problematic, which is currently becoming widespread in the Czech research environment. In contrast to the previous empirical studies, this thesis is devoted to the thematic map skills and their level of development among students of grammar schools and geographically oriented branches of university studies. Due to the significant differences between used cartographic methods of visualization on thematic maps the main aim of this diploma thesis is to identify and explain used cartographic method's influence on the level of these important skills. To achieve this aim, it was necessary to focus on different parts of the curriculum (intended, implemented, learned) and to use several scientific methods because this topic hasn't been well established yet and therefore only few theoretical and empirical studies dealt with this specific issue. Firstly, the review of literature focused on thematic cartography, map skills and on identifying their level of development among students was done. Due to the lack of specialized resources the model of thematic map skills was created. This specific model was further used during didactic test's designing and also for its results' interpretation. Similarly for the creation of appropriate didactic test -...
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Proteomic analysis of selected oncohematological diseases
Pimková, Kristýna ; Petrák, Jiří (referee)
Oxidative stress is an important factor in carcinogenesis of oncohematological diseases. However its role in the pathogenesis of myelodysplastic syndromes (MDS) remains unclear. In this study, we have determined the oxidative status and evaluated proteomic changes in plasma of MDS patients as a consequence of oxidative dysbalance (oxidative modifications, protein-protein interaction and complex forming). We measured the levels of total cysteine, homocysteine, cysteinyglycine, glutathione, nitrites and nitrates in the plasma from 61 MDS patients and 23 healthy donors using high performance liquid chromatography. Glutathione and nitrites levels reduced significantly while other aminothiols levels increased significantly in plasma of MDS patients. This association with oxidative stress did not correlate with iron overload. We also found enhanced levels of asymmetric dimethylarginine in serums of middle aged patients with MDS that correlate to posttranslational modifications of proteins arginyl residues. Furthermore, carbonylated proteins level was significantly elevated in MDS patients compared to healthy donors. Using mass spectrometry, 5 S-nitrosylated blood platelets proteins were identified in plasma and blood platelets of MDS patients and set of 16 plasma proteins with high probability of...
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New trends in international commercial arbitration
Jakoubek, Lukáš ; Brodec, Jan (referee) ; Pfeiffer, Magdalena (referee)
This thesis deals with the latest trends in international commercial arbitration. Specifically, it focuses on transparency in international investment arbitration and third-party funding in international commercial arbitration. The objective of this thesis is to provide a comprehensive interpretation of a gradual development of these trends and to also give potential Czech readers an idea about present hot topics in international arbitration coming mostly from foreign sources of bibliography. The thesis is divided into three chapters. The first chapter dedicates itself to defining arbitration in the context of various dispute resolution methods and provides its basic comparison to international arbitration. At the same time, it offers an insight into the advantages and disadvantages of this type of dispute resolution and it discusses the principles of confidentiality and privacy of arbitration proceedings. The second chapter is divided into two main parts. The first one is focused on defining key terms of the specific area of international investment arbitration, including different regimes of treatment of international investments in order to protect them. It also includes a detailed description of the most important sources of international investment law and further discusses ways to resolve...
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Development and applications of affinity carrier for isolation of human carbonyl-reducing enzymes
Andrýs, Rudolf ; Wsól, Vladimír (advisor) ; Šebela, Marek (referee) ; Šatínský, Dalibor (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Rudolf Andrýs Supervisor: Prof. Ing. Vladimír Wsól, Ph.D Title of dissertation thesis: DEVELOPMENT AND APPLICATION OF AFFINITY CARRIER FOR ISOLATION OF HUMAN CARBONYL-REDUCING ENZYMES For several millennia the human medicine is based on application of small bioactive molecules that are administered in the form of plant extracts or synthetic compounds. However, their use in modern medicine is not possible without a detailed understanding of their biochemical effects and identification of their molecular targets. Chemical proteomics based on the specific recognition between the bioactive molecule and the target molecule is currently the most widely used techniques for identification of molecular targets of small molecules. Compared to conventional biochemical methods (e.g. 2D electrophoresis), chemical proteomics represents particularly sensitive and very selective technique that enable successful identification of biomolecules from complex biological samples that are naturally presented in very small concentrations. Carbonyl- reducing enzymes, which play an important role in physiology due to their involvement in metabolism of various endogenous (e.g. prostaglandins, steroid...
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In vitro evaluation of photodynamic activity of tetrapyridoporphyrazine derivatives for treatment of solid tumours.
Jedličková, Adéla ; Macháček, Miloslav (advisor) ; Šimůnek, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradci Králové Department of Biochemical Sciences Candidate: Bc. Adéla Jedličková Supervisor: Mgr. Miloslav Macháček Title of diploma thesis: In vitro evaluation of photodynamic activity of tetrapyridoporphyrazine derivatives for treatment of solid tumors Nowadays, cancerous diseases belongs among the main causes of death, incidence continues to rise. Therefore research in the area of tumor treatment significantly focuses on the development of new potential anticancer drugs and therapeutical approaches. Photodynamic therapy (PDT) belongs to clinically approved therapeutic methods for the treatment of the malignant and nonmalignant diseases. PDT is composed of three main components - photosensitiser (PS), light and oxygen, all of them are non-toxic on their own. However, their interaction leads to a generation of highly reactive molecules. This method is based on absorption of light with appropriate wavelenght by PS and subsequent energy transfer from photon to surrounding molecules, especially to oxygen. This transfer leads to a generation of cytotoxic reactive oxygen species (ROS) of which singlet oxygen plays a main role. ROS subsequently causes a damage of surrounding cell structures, which leads to irreversible damage of cell functions and...
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Metabolism of anthelmintics in plants
Pavlík, František ; Szotáková, Barbora (advisor) ; Vokřál, Ivan (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: František Pavlík Supervisor: Prof. Ing. Barbora Szotáková, Ph.D. Consultant: RNDr. Radka Podlipná, Ph.D. Title of diploma thesis: Metabolism of anthelmintics in plants Anthelmintics enter into environment mainly through excretion of treated animals. They may cause a lot of adverse effects in ecosystem as they can affect non- target animals or they can accumulate in plants. Further, after consumption of these plants by livestock on pastures the resistence of helminths can be induced. Therefore, the present study was designed to find out biotransformation of frequently used anthelmintics flubendazole (FLU), albendazole (ABZ), fenbendazole (FBZ), ivermectine (IVM) and monepantel (MOP) by the common meadow plants harebell (Campanula rotundifolia) and plantain (Plantago lanceolata), which may often come into contact with anthelmintics through the excrements of treated animals. As model systems, suspensions of harebell and plantain cells and in vitro regenerants of plantain were used. There was no toxic effect of anthelmintics for these plants; they were able to metabolize anthelmintics into a wide scale of various compounds. These metabolites were identified by ultrahigh-performance liquid...
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Vliv vybraných látek ovlivňujících centrální nervový systém na kostní metabolismus
Fekete, Soňa ; Živný, Pavel (advisor) ; Horák, Petr (referee) ; Nobilis, Milan (referee)
The increase in life expectancy of the world population is associated with challenges regarding health issues. For instance, osteoporosis is a medical condition mostly observed in elderly people, in which the quality and quantity of the bone are severely affected. Not only for women but also for men, osteoporosis is recognized as an important public health issue. Osteoporosis is a systemic skeletal disorder and is a result of loss of skeletal mass. Osteoporosis is characterized by low bone mass, microarchitectural deterioration of bone tissue and an increase in bone fragility and susceptibility to fracture. Osteoporotic fractures are a significant cause of morbidity and mortality. The longterm use of drugs such as antiepileptics and antidepressants could affect the onset of osteoporosis. The aim of the study was to evaluate the effect of orchidectomy, the effect of newer antiepileptic (levetiracetam, lacosamide, topiramat, lamotrigine) and antidepressive drugs (mirtazapine, venlafaxine and trazodone) on bone metabolism in healthy male Wistar rats. The first specific aim was to determine the effect of orchidectomy on bone metabolism in rats. We found that after 12 weeks post-orchidectomy there was a negative effect on bone metabolism in rats. These results established these animals as suitable models for...
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Reference gene selection for mRNA quantification in Haemonchus contortus
Růžičková, Michaela ; Matoušková, Petra (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Michaela Růžičková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Reference gene selection for mRNA quantification in Haemonchus contortus Haemonchus contortus is a parasitic nematode, whose multiresistance to ant- helmintics means global problem, threatening mostly small ruminants farming. Genom and transcriptom have been published in 2013, allowing gene expression studies to be conducted. Reference genes for this studies have not been validated yet. Use of suitable reference genes is essential for accurate normalization of gene expression levels. Aim of this work was to identify and validate potential reference genes for gene expression studies in H. contortus adults. Eleven genes were chosen, stability of their expression was assessed in males and females of two H. contortus strains, one drug-susceptible (ISE) and one multi-drug-resistant (WR). Total RNA was extracted and reverse trancribed to cDNA. cDNA was diluted and analyzed using quantitative real-time PCR with SYBR Green I detection. Expression stability was evaluated by computer programs BestKeeper, geNorm, NormFinder and the comparative CT method. Ncbp, ama, sodc, gapdh and farb were found to be most stable...
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