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Distributed Computing by force of Action Script
Minář, Petr ; Kuba,, Martin (referee) ; Matoušek, Radomil (advisor)
This Master thesis deals with designing and realization of optimalization software in ActionScript 3.0 background. The designed applications realized in terms of computation by standalone and distributed variant and by means of chosen heuristic method called HC12. For verification of a function and performance was select set of testing examples. The main purpose of the work was create public clients in the network internet and calculated partial computing by it. The completed clients are selected by competence and carry on tasks. This research has been supported by the Czech Ministry of Education in the frame of MSM 0021630529 Research Intention Intelligent Systems in Automation.
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Effect of optical connectors quality on transmission
Bernkopf, Miroslav ; Křepelka, Václav (referee) ; Filka, Miloslav (advisor)
This diploma thesis deals with optic fiber connectors and their influence on transmissions realized via fiber optics. Optical fibers and lightguides play the key role in telecommunications because of constant demands of higher bandwidth and data rates. Original metalic wires do not meet market requirements and so the fiber optics are more and more being used. There are various types of fibers and there are also many ways of connections. This work deals with demountable way of connection – optical connectors, which have the biggest influence on a total attenuation and route length. Connector has to meet various requirements such as cleannes, quality, mechanical construction and ferrule polishing.
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Gene sequencing in patients with cancer anamnesis
MARKOVÁ, Iveta
Cancer is the second most common cause of death in the Czech Republic, of which 5-10% are occupied by hereditary cancer syndromes. They are caused by a congenital - hereditary mutation in one of the alleles of the genes, when after the second random intervention in the other allele hereditary cancer develops. It is important to distinguish between hereditary and sporadic carcinomas due to the high risk of inheritance of mutated alleles in the family. The indication may be, for example, the onset of the disease at a young age or the recurrence of the cancer in the family In my work I focused on the analysis and evaluation of data obtained by Sanger sequencing. The aim was to find mutations in the genes mentioned below and to evaluate their pathogenicity by comparison with databases. In the theoretical part of the bachelor thesis I deal with cancer in general and hereditary cancer. I specify the hereditary breast and ovarian cancer syndrome, including genes, that may cause this syndrome - BRCA1, BRCA2, TP53, PTEN, ATM, PALB2, I also deal with Lynch syndrome and the MMR gene system. Last but not least, I describe a familial adenomatous polyposis associated with the APC gene. In the research part I focused on the examination of selected areas of 18 anonymized samples in the gene PALB2 - exon 13 and in the gene BRCA2 - exon 10/4 and exon 11/12. Using the PCR method, I prepared the samples for Sanger sequencing, which then took place in GenSeq s.r.o. In the last part of my work I deal with the analysis and evaluation of the results using the BioEdit program and the NCBI database. I found a mutation in 5 samples - in 4 it was a deletion of one nucleotide with a conflicting interpretation of pathogenicity, the last mutation was pathogenic - causes hereditary breast and ovarian cancer syndrome, it was a nucleotide duplication.
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MHC II-EGFP knock-in mouse model as a suitable tool for quantitative gut immunology under conventional and germ-free conditions
Tušková, Liliana ; Černý, Jan (advisor) ; Filipp, Dominik (referee)
Germ-free animals have been used to study the effects of microbiota for several decades. In that time, numbers of differences from specific-pathogen-free (SPF) animals have been reported, including differences in absolute numbers or percentages of various immune populations, enormously enlarged coecum and lack of germinal centers. However, many of the crucial information about structural and functional differences in their secondary lymphoid organs still remains uncovered. With novel microscopical approaches, such as light sheet fluorescent microscopy, enabling 3D visualization of whole samples without processing them to a series of slides, and multicolor cytometry, allowing the characterization of numbers of cellular populations within a matter of seconds and in a highly quantitative manner, the uncovering of fundamental differences finally seems to be within reach. MHC II-EGFP knock-in mouse model brings the advantages of a fluorescent protein expressed in physiological histological contexts into both fields. Lymphoid and other tissues can be visualized microscopically without the need of staining (even in vivo). Information about the expression of both plasma membrane-localized and intracellular MHC II in various tissues could be acquired directly. Combining MHC II-EGFP knock-in mouse model with...
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Intestinal cancer and mouse models of the disease
Baloghová, Nikol ; Janečková, Lucie (advisor) ; Gemperle, Jakub (referee)
Intestinal cancer is a serious and common disease. To understand the mechanisms of its development, it is important to know the structure of the intestinal epithelium, as well as the signalling pathways that maintain the homeostasis and regulate cell proliferation and differentiation. Development of the intestinal cancer is a multistep process in which many molecular events underlie initiation and progression of the disease. Transgenic mice produced by genetic engineering are essential tools in both research of the intestinal cancer initiation and progression and possible treatment strategies. The aim of this work is to describe the intestinal anatomy and the renewal of the intestinal epithelium including the role of multiple signalling pathways, to summarize the most common mutations conditioning human colorectal carcinoma development and to define the existing mouse models of the disease.
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Distributed Computing by force of Action Script
Minář, Petr ; Kuba,, Martin (referee) ; Matoušek, Radomil (advisor)
This Master thesis deals with designing and realization of optimalization software in ActionScript 3.0 background. The designed applications realized in terms of computation by standalone and distributed variant and by means of chosen heuristic method called HC12. For verification of a function and performance was select set of testing examples. The main purpose of the work was create public clients in the network internet and calculated partial computing by it. The completed clients are selected by competence and carry on tasks. This research has been supported by the Czech Ministry of Education in the frame of MSM 0021630529 Research Intention Intelligent Systems in Automation.
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Effect of optical connectors quality on transmission
Bernkopf, Miroslav ; Křepelka, Václav (referee) ; Filka, Miloslav (advisor)
This diploma thesis deals with optic fiber connectors and their influence on transmissions realized via fiber optics. Optical fibers and lightguides play the key role in telecommunications because of constant demands of higher bandwidth and data rates. Original metalic wires do not meet market requirements and so the fiber optics are more and more being used. There are various types of fibers and there are also many ways of connections. This work deals with demountable way of connection – optical connectors, which have the biggest influence on a total attenuation and route length. Connector has to meet various requirements such as cleannes, quality, mechanical construction and ferrule polishing.
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