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National Repository of Grey Literature

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	GlcNAc8-dendrimer as a ligand of NKR-P1A receptor in effector-target cell interaction and G-protein signaling Svoboda, Jan ; Kuldová, Markéta ; Huliková, Katarína ; Miller, R. G. ; Fišerová, Anna GlcNAc8 glycodendrimer is a specific ligand of rat NKR-P1A receptor isoform. In effector cells triggers the Gq/11 protein-mediated signaling pathway, and induces subsequent changes in tumor microenvironment Detailed record
	The role of A2A adenosine receptor in NK cell-mediated cytotoxicity Kuldová, Markéta ; Svoboda, Jan ; Kovářů, H. ; Kovářů, F. ; Šplíchal, Igor ; Fišerová, Anna Adenosine receptor, NK cell, immunity systém, cytotoxicity, Salmonella typhimurium, colorectal carcinoma, murine model, newborn pigs model Detailed record
	Úloha A2A adenozínového receptoru v NK buněčné cytotoxicitě Pompach, Petr ; Vančurová, Markéta ; Man, Petr ; Novák, Petr ; Bezouška, Karel After studies with monoclonal antibodies, we would like to focus on the identification of proteins associated with membrane microdomains of human NK cells by mass spectrometry Detailed record
	Multivalentní glykomika v imunoterapii experimentálních nádorů Vannucci, Luca ; Pospíšil, Miloslav ; Krist, Pavel ; Křen, Vladimír ; Huliková, Katarína ; Luptovcová, Martina ; Svoboda, Jan ; Kuldová, Markéta ; Bezouška, Karel ; Rossmann, Pavel ; Difato, Francesco ; Mosca, F. ; Fišerová, Anna Lectin-like receptors can modulate the cytotoxic, regulatory and dendritic cell responses by their interactions. GN8 multivalent glycoconjugates are able to internalize inside and activate NKR-P1 positive cells inducing production of IFN-gamma and expression of CD69. The final effect is a modulation of anticancer immunity with reduction of cancer growth Detailed record
	Rozvoj kolorektálního karcinomu u konvenčních a bezmikrobních potkanů: imunologické a enzymatické aspekty Vannucci, Luca ; Štěpánková, Renata ; Pospíšil, Miloslav ; Kozáková, Hana ; Tlaskalová, Helena ; Kuldová, Markéta ; Fišerová, Anna Environmental conditions cooperate to colorectal carcinogenesis. In an experimental model in the rat was evaluated the impact of the commensal intestinal flora on cancer development, and consequent general variations of the immunity, versus germ-free (GF) conditions. Less and smaller tumors developed in GF rats and a better developed immune response appeared to be able to be elicited in absence of intestinal bacteria Detailed record
	Cell sorting in practise Cimburek, Zdeněk ; Horváth, Ondřej ; Růžičková, Š. ; Fišerová, Anna Cell sorting is used for prepare cell clones, functional studies, clonal analyzis, expression studies. We are using FACS sort for positive and negative sorting, for single cells cloning and in some cases is used immunomagnetic presort Detailed record
	Úloha sacharidových struktur v NKR-P1 zprostředkované interakci efektorových a cílových buněk Fišerová, Anna ; Svoboda, J. ; Kuldová, Markéta ; Pucová, Alexandra ; Bezouška, Karel ; Pesta, M. ; Vannucci, Luca ; Pospíšil, Miloslav Masking oligosaccharide sequences can assist transformed cells to escape immune surveillance. N-acetyl-glucosamine coated dendrimers have binding affinity for NKR-P1 receptor and can also modify cancer cell glycosylation profile. Effects of treatment on immune cell subpopulations were analyzed in blood, lymphoid tissues and organs, and inside tumor in B16F10 melanoma bearing mice. Treatment enhanced tumor cell immunogenicity, and induced maturation,differentiation and homing of cytotoxic cells to tumor microenvironment. Detailed record

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