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Inquiry methods in educational area Man and his world: Seeds, fruit and young plants
Nikrýnová, Alena ; Stará, Jana (advisor) ; Straková, Jana (referee)
This diploma work deals with the issues of inquiry methods of teaching at primary schools. The theoretical part provides some reflections on contributions and limits of inquiry methods and defines basic inquiry skills. Activities concerning the theme "seeds, fruit, young plants" for the pupils of the 1.-4.grade are suggested in the practical part. Nine of them were tried out in classes. The results of the action research are summarized in the conclusion. They are related to these research questions: 1. How to adjust inquiry activities in a suitable way for their use in the given classes at primary schools? 2. How do children react to this work style? 3. What demands does this work style make on teachers? 4. What problems concerning partial inquiry skills could emerge? How to continue working at them?
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Study of the regulation of phosphoenolpyruvate carboxylase activity in higher plants
Škrletová, Denisa ; Ryšlavá, Helena (advisor) ; Müller, Karel (referee)
Phosphoenolpyruvate carboxylase (EC 4.1.1.31; PEPC) is one of the carbon dioxide- fixing enzymes, which yields oxaloacetate from phosphoenolpyruvate and bicarbonate. Regulation of PEPC activity occurs at many levels. In addition to pH and concentration of activators and inhibitors, it is phosphorylation as well. Phosphorylation of PEPC causes a change of kinetic parameters, such as maximal reaction rate, sensitivity to activation or inhibition. Considering that, there is still little information like this about C3 plants and that regulation is in various plant species different, I have dealt with monitoring of the kinetic parameters and regulation possibilities of PEPC isolated from C3 plant sources (Cannabis sativa L., Chenopodium quinoa, Pisum sativum L., Lens culinaris). While the activity of PEPC from leaves of Cannabis sativa L. was decreased by alkaline phosphatase, the activity of PEPC from seeds of Chenopodium quinoa, Pisum sativum L., Lens culinaris was not affected by alkaline phosphatase. The affinity of PEPC from seeds Chenopodium quinoa, Pisum sativum L., Lens culinaris to the substrate PEP was higher than in the case of PEPC from leaves of Cannabis sativa L.. For PEPC from Cannabis sativa L. was found that the apparent dephosphorylation leads to decrease of sensitivity to the...
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Analysis of biological features of Capsella bursa-pastoris (L.) Med.
Schneebergerová, Tereza ; Hamouz, Pavel (advisor) ; Holec, Josef (referee)
The aim of this bachelor thesis is to determinate selected biological characteristics of shepherd´s-purse, which have impact on the establishment and expansion of its populations in field crops. In particular, development of primary dormancy was studied and the effect of factors that influence the dormancy and germination of seeds was determined.
Shepard´s purse is an annual or biennial herb of the cabbage family, which is classified as winter annual weeds. Primarily infest the winter and spring crops, rapeseeds, potatoes or forages. It appears from lowland to foothills, so biological features of individual population can be very different. The ways of control are shallow tillage or herbicidal treatment.
After harvesting of ripe seed the first experiment was set to define the primary dormancy. Remaining seeds were stored in two different types of environment. Part of seeds was stored in the dry at 20 °C. Others seeds were stratified at 5 °C. Effect of storage conditions on primary dormancy was tested every two weeks. Seeds were germinated for
1 week in grow chamber under the influence of various factors such as different light mode, type of water and type of media for germination.
The results were processed by multiple-factor analysis of variance. Fresh seeds showed a strong primary dormancy at constant temperatures of 10 °C and 20 °C. Treatment with fluctuating temperature (20/10 °C), filter paper, drinking water and stored in dark had the highest germination rate (10.75 %). Seeds stratified at 5 °C had increase in germination in first weeks, but lost it during storage. On the other hand, seeds stored in dry at 20 °C firstly were in dormancy, but they lost it gradually during storage.
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@Potential of brassinosteroids for forest seed and nursery management and for forest regeneration@
Bach, Petr ; Kuneš, Ivan (advisor) ; Švec, Otakar (referee)
Brassinosteroids (BRs) are a class of polyhydroxysteroids that have been recognized as a sixth class of plant hormones. In specific conditions BRs can positively influence the plant growth and development. The first isolated brassinosteroid was natural Brassinolide from rapeseed (Brassica napus L.). Recently BRs are extracted primarily synthetically; they are effective even in very low concentrations. Several studies of different plant species show positive influence of BRs on growth, flowering, germination and resistance to stress factors. Existing scientific studies that deal with BRs impact on plants were predominantly applied on agricultural crops. Until presence, the similar research in forest seed treatment and forest nursery was almost missing. In the Czech Republic the Department of Silviculture of the Faculty of Forestry and Wood Sciences of the Czech University of Life Sciences Prague newly started to deal with the use of BRs in forestry. The aim of this master thesis is to elaborate literature review on BRs and to summarize existing research of their impact on plants. Second part of the thesis solves influence of BRs on Scots pine (Pinus silvestris) seeds germination. Tests are performed in normal and in stress conditions. Results of the practical part show that BRs affect positively seed germination in normal conditions; seeds treated with BRs show higher germination rate than control seeds. Positive impact was not registered when the seeds were exposed to stress factor -- heat. Any research paper showing significant positive influence on forest seedlings in Central European conditions was not published yet.
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Biology, seed characteristics and growth establishment of comfrey (\kur{Symphytum officinale }L.)
KOVÁŘOVÁ, Jaroslava
The thesis titled "Biology, seed characteristics and growth establishment of common comfrey (Symphytum officinale L.)" dealt with the evaluation methods of reproduction comfrey and evaluation of the impact of environmental conditions to the grow of comfrey. Further it dealt with germination tests and their statistical evaluation while respecting the specific work with seeds. They were evaluated seed characteristics, which are reflected in the growth of these plants arising both in soil and in a laboratory environment. Literary part gave information about biology, characteristics, morphology, prevention and regulation of comfrey even more its cultivation, nutrition of the grassland and seed characteristics, germination and dormancy. In the final section of work was monitored coverage, botanical images, representation of herbaceous species growing near comfrey. The study also discussed vernalization, size of comfrey leaves, growth and development depending on the content of nutrients (soil fertility) and water in the soil and on the number of cuts to its growth. There were designed a suitable ways of reproduction and growing of comfrey.
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Content of toxic and esential elements in bio foodstuffs and their comparing with ordinary foodstuffs
Kohoutková, Nina ; Diviš, Pavel (referee) ; Vitoulová, Eva (advisor)
The aim of this study is to determine the content of toxic and essentials elements in samples of nuts and seeds produced by ecologic agriculture and to compare results with nuts and seeds from conventional agriculture production. These nuts and seeds can be bought in the Czech market (except bio pine nuts, bio pecan nuts and bio pistachios). The amount of elements was analysed using inductively coupled plasma mass spectrometry after previous digestion of samples using microwaves. This study summarizes the main physiological functions of toxic and essentials elements. Results were also compared with another studies. It does not results from all measured amounts of all essential elements, that bio nuts and seeds have higher amounts of these elements; however, all amounts of toxic elements in bio nuts and seeds are lower than in the no-bio ones. For more precise results it would be necessary to measure more samples.
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The evaluation of seed properties of Lathyrus pratensis and Vicia cracca and their employment in permanent grasslands.
KRATOCHVÍLOVÁ, Eva
The theme of the thesis are climbing legumes meadow vetchling (Lathyrus pratensis) and tufted vetch (Vicia cracca). The literature search defines watched kinds of legumes and their occurence in permanent grassland. There is summarized seed growing of legumes because of its problematic production. Legumes are valuable components of grassland especially thanks to fixation of nitrogen and high capacity of proteins. The practical part is focused on observation of floristic compilation of watched locations for three years. Particular locations were compared from the point of view of changes in land management and weather. Pods and seeds of observed kinds were collected and evaluated in single years. Experiments of seed's field germination were done after different period from picking and in case of various treatment.
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The effects of low-temperature plasma on initial growth of Brassica napus L. and Zea mays L.
LANDOVÁ, Tereza
Curently seed treatment with low-temperature plasma is one of the trends in alternative agriculture. This trend is based on a seed stimulation without the use of chemical stimulants. The subject of this thesis is the study the effects of low-temperature plasma on initial growth of selected agricultural crops Zea mays L. and Brassica napus L.. The length of sprouts and roots were measured from the initial germination. Growing biomass was dried and weighed at the end of each experiments. Discussion is focused on the time of plasma treatment in relation to possible profitable crops.
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Vliv růstových regulátorů na proteom rostlin v průběhu klíčení
Habánová, Hana
Seed germination is a complex process during which an embryonic plant contained within a seed transforms into a seedling. The majority of species, including model plant Arabidopsis thaliana produce dry seeds that will fully re-establish metabolism after imbibition. This early phase of a plant's life is highly important for its survival and its progress depends on both internal and external stimuli. This thesis entitled "Effects of plant growth regulators on proteome dynamics during seed germination" is focused on effects of light and plant growth regulators on germination. To get an insight into molecular mechanism of germination, proteome dynamics in germinating Arabidopsis were investigated. Seeds were imbibed in presence of plant growth regulators in continuous light or dark and material for LC-MS proteome profiling was harvested after 24 and 48 hours. Altogether, abundances of more than 1100 proteins were followed. PCA analysis and a detailed pair-wise comparison of mock-treated seeds provided evidence that the modern proteome-wide analysis is a promising tool for plant germination research.
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