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A novel function of red pigment-concentating hormone in crustaceans: \kur{Porcellio scaber} (Isopoda) as a model species
ZRALÁ, Jana
The HPLC and LC/MS analyses of the CNS from isopod crustacean the woodlouse, Porcellio scaber revealed a presence of the red pigment-concentrating hormone (Panbo-RPCH) in this species. It has been shown that this neuropeptide plays a role in mobilization of energy stores: topical treatments of P. scaber individuals by Panbo-RPCH in a concentration 20 pmol/{$\mu$}l increased the level of glucose in haemolymph about 4 times. Glucose was the main carbohydrate mobilized by the Panbo-RPCH treatment. Despite the demonstration of hyperglycaemic activity of Panbo-RPCH, no stimulatory effect of this hormone on the locomotory activity of P. scaber was observed. The present study is the first discovery of an occurrence of Panbo-RPCH and its hyperglycaemic activity in the representative of the isopod crustacean.
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Analysis of the role of PilA proteins in the cyanobacterium \kur{Synechocystis} sp. PCC 6803.
LINHARTOVÁ, Markéta
The PilA proteins are major components of pili fibres which are essential for motility in bacteria. This project was focused on the role of the PilA proteins in cyanobacteria, specifically in the alternative functions that are not directly related to motility. The major task of this project was also to develop a method for the purification of the PilA1 protein from Synechocystis under native conditions and to analyze purified PilA1 protein in detail.
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The problems of the sick with hyperlipidemia and the influence of this disease on their life
DOUSKOVÁ, Renata
Hyperlipidemia means elevated content of lipids in the blood. The work was aimed at problems of the patients with hyperlipidemia, and at the influence of the illness on their lifestyle. The work consists of a theoretical part and a practical survey. The research took place in specialized surgeries at the Railway Hospital Prague. Two hypotheses were proposed on the basis of the theoretical part, and neither of them was affirmed. The results acquired on the basis of the questionnaire research will be given to the specialized surgeries where the survey took place. The aim has been fulfilled because of the fact that hyperlipidemia influences the lifestyle of patients.
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The effect of fertilization by nitrogen on leaves of free amino acids in plants
Neuberg, M. ; Pavlík, Milan ; Balík, J. ; Kaliszová, R. ; Pavlíková, D.
The effect of different fertilization systems, especially CULTAN (Controlled Uptake Long Term Ammonium Nutrition), on levels of selected amino acids (alanine, glycine, serine, asparagine and glutamine) was investigated in this study. As modeling plant was used maize (Zea mays L.; hybrid Rivaldo) and levels of amino acid were measured by GC-MS. We found significant difference in the levels of certain amino acids. Concentration of alanine and glutamine was higher by treatment with urea ammonium nitrate solution (DAM 390) compared with individuals treated by ammonium nitrate.
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The phosphorus role in plants
Pavlíková, D. ; Pavlík, Milan ; Balík, J.
Phosphorus play very important role in plant metabolism. It is active as energy transporter and important structural component. Phosphate is relatively unavailable to plant roots. Thus plants have evolved several strategies for obtaining P from the soil. They can modify root structure and function, excrete root exudates helping with solubilization of P compounds in root rhizosphere.
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Participation of electrochemistry in the studies of human metabolism
Navrátil, Tomáš ; Šenholdová, Z. ; Přistoupilová, K. ; Přistoupil, T. I. ; Heyrovský, Michael ; Pelclová, D. ; Žák, J.
The contribution deals with explanation of metabolism of long term supplemented creatine in human organism. This process differs from metabolism of natural endogenous creatine, which is excreted in form of creatinine. Exogenous creatine induces increase of peptide formation, of body mass, and of intracellular fluid.
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Electrochemical study of the effect of long-term creatine supplementation creatine on human metabolism
Šenholdová, Z. ; Navrátil, Tomáš ; Heyrovský, Michael ; Pelclová, D.
The volunteers were supplemented in time interval of one month by creatine. Body parameters (mass, height, intra- and extracellular water content etc.), levels of vitamins and a few other compounds in urine and in blood (vitamine B12, homocysteine, thiodiglycolic acid, creatine, creatinine etc.) were followed. The corresponding metabolic processes are studied.
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Creatine fortification in relation to homocysteinemia
Kohlíková, E. ; Petr, M. ; Šenholdová, Z. ; Navrátil, Tomáš ; Heyrovský, Michael ; Přistoupilová, K. ; Přistoupil, T. I.
Some people supplement their diet by high doses of creatine (CR) to increase their muscle performace. In this way, they intensify the biochemical processes on cell membranes. The water thus formed is retained in the cells, which increases the body mass. The organism must remove exogenously added CR, probably in a similar way as in removing xenobiotics via oxidation with cyt.P450 to 2C units to cooperation with GSH. Our new simple and cheap voltammetric method enables to estimate thiogycolic (TDGA), the natural product of oxidative catabolism of thiocompounds, excreted to urine in regular catabolic processes.
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