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Caring for patients colonized with resistant microbial strains
Poláková, Stanislava ; Heczková, Jana (advisor) ; Novotná, Jindra (referee)
This thesis is focused on the complex nursing care of patients colonized with the resistant microbial strains. In the theoretical section I describe the major events of the history, the current issue of the antibiotic treatment and the species of occurring resistant strains of microbes. It also deals with recommended procedures in the care for these patients, such as patient isolation, use of the protective equipment, handling of the biological material , individualization of the aids, handling of the linen and the principles of health care. It also includes preventing the spread of disease resistant strains of the microorganisms and patient recolonization. The empirical part consists of the quantitative research conducted at the University Hospital in Pilsen with the help of a questionnaire survey. The questionnaires will be distributed to the nurses in intensive care units without the single - patient box system. The aim of the research is to determine nurses' knowledge of the disease resistant microbial strains and the ways they create patient isolation. Evaluation questionnaires will provide the pieces of information to compare the individual practices and knowledge of nurses in intensive care units. Results of this work can be used as a source of information or the study material. The benefit...
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Analysis of the Use of Antibiotics in the United Arab Emirates
Abasaeed Elhag, Abobakr ; Vlček, Jiří (advisor) ; Kolář, Jozef (referee) ; Urbánek, Karel (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Social and Clinical Pharmacy Candidate PharmDr. Abobakr Abasaeed Elhag Supervisor prof. RNDr. Jiří Vlček, CSc. Title of Doctoral Thesis Analysis of the Use of Antibiotics in the United Arab Emirates Introduction and Aims: The misuse of antibiotics for viral infections (for which they are of no value) and the immoderate use of broad spectrum antibiotics instead of narrower spectrum antibiotics have been well-documented and reported. Therefore the inappropriate use of antibiotics is getting a global problem, mainly in the developing countries. Emergence of antibiotics resistance is often a result of irrational prescribing patterns, misuse of the antibiotics as well as self-medication. Although the United Arab Emirates (UAE]) antimicrobial policy restricts dispensing of antibiotics without prescription , studies revealed the wide availability of these agents over the counter (OTC), their over prescribing pattern and the high prevalence of self-medication with antibiotics, unfortunately and regardless of this results, there is a lack and paucity in studies that tracking the prevalence of antibiotics resistance. The aim of our research was to evaluate the attitude of the community towards the use of antibiotics, estimate...
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Analysis of self-medication with antibiotics in Kosovo
Veseli, Ardita ; Vlček, Jiří (advisor) ; Kostřiba, Jan (referee)
Title: Analysis of self-medication with antibiotics in Kosovo Student: Ardita Veseli Supervisor: Prof. PharmDr. Jiří Vlček, Ph.D. Specialist Supervisor: Mgr. T. Belkina Department of Social and Clinical Pharmacy, Charles University in Prague, Faculty of Pharmacy in Hradec Králové Background: Self-medication is described as a behavior during which someone utilizes drugs for the purpose of treating self-diagnosed symptoms or disorders or the intermittent or continued use of a prescribed drug for chronic or recurrent disease or symptoms. When certain individuals self-medicate with antibiotics, they do it without any prior consultation with a physician. The antibiotics are bought from community pharmacies without a prescription, left-over antibiotics are used or they simply decide to follow the advice given from family members, neighbors or friends. Even though antibiotics are considered revolutionary therapeutic agents in order to eradicate microbes, the repeated and improper antibiotic consumption is contributing to the increased prevalence of antibiotic resistance which nowadays is one of the world's most pressing health problems. Aim: The main goal of the theoretical part is to describe self-medication with antibiotics, the patterns influencing it and some complications associated with this...
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Role of extracellular DNA in microbial biofilms
Gromov, Anton ; Chytilová, Aneta (referee) ; Obruča, Stanislav (advisor)
Abstract This bachelor’s thesis deals with defining the role of extracellular DNA in microbial biofilms. The thesis includes a part about the basic characteristics and functions of biofilms, as well as the characteristics and functions of extracellular DNA. The theoretic part additionally deals with the process of formation of biofilms, and the influence of different types of stresses on it. Also, the paper discusses the role of such stresses. The role of biofilm in bacteria and their reproduction methods are also discussed. An interesting section of the thesis outlines the impact of antibiotics on biofilms, or the ways to gain DNA or use DNase. The eDNA functions and their effects on bacterial defence will be discussed thoroughly. In the experimental part, the biofilm content was examined and compared with cells growing in a liquid and solid medium. Measurement of DNA content in media was carried out and the effects of salt and temperature stresses on biofilm were also measured. Stress conditions affected the production of PHB, which is used as a reserve form of energy and carbon. Total sugars were measured using an anthracite reagent to study the effects of stresses on the content of macromolecules. The presence of eDNA in biofilms and planktonic cells was firstly checked using gel electrophoresis and then accurate concentrations in planktonic and biofilm cells were determined using different spectrophotometric methods.
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Liquid Chromatography Methods for Analysis of Actinomycete Secondary Metabolites - Potential Antibiotics
Kameník, Zdeněk ; Nesměrák, Karel (advisor) ; Cvačka, Josef (referee) ; Bosáková, Zuzana (referee)
(EN) This dissertation thesis contains scientific results achieved in the field of analytical chemistry, particularly liquid chromatography. The major part of the results has been published in prestigious international journals in five papers. In addition to that, relevant yet unpublished results have been included as well. In general terms, the work presented here contributed to the concerted efforts to tackle the current lack of novel antibiotics. Specifically, high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) techniques coupled to a variety of detection systems have been employed for analysis of antibiotics and actinomycete secondary metabolites. The first thematic part describes the development of liquid chromatography methods for analysis of lincomycin precursors, lincomycin precursor analogues, and lincomycin derivatives. The methods have been applied to study lincomycin biosynthetic pathway and obtain improved lincomycin derivatives by mutasynthesis. The second thematic part aims at investigating alternative approaches for analysis of antibiotics. Firstly, the core-shell particle and the sub-2 μm particle chromatographic columns were compared. The core-shell particle columns compatible with HPLC proved to be a convenient alternative to the...
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The introduction of diagnosis of new bacterial pathogens Ralstonia a Achromobacter isolated from patients with cystic fibrosis and determining their sensitivity to antibiotics
Michálková, Alice ; Melter, Oto (advisor) ; Bébrová, Eliška (referee)
Cystic fibrosis (mucoviscidosis) is an incurable genetic disease caused by mutations in the CFTR (Cystic Fibrosis Transmembrane Conductance Regulator Gene). The most common causes of increased mortality and morbidity of patients include bacterial respiratory infections which may occur even due to less frequent pathogens. Bacterial species of the Ralstonia and Achromobacter genera are not considered pathogenic for healthy people, but they have been established as pathogens in the sputum of patients with CF. However, due to their phenotypic similarity to other bacterial pathogens encountered in patients with CF, microbiologists often do not pay attention to them. The aim of this thesis was to propose some methods of identification of the genus Ralstonia, to determine both quantitative and qualitative susceptibility towards antibiotics, and to make a bibliographical search focused on the issue of the Achromobacter genus. Strains of Ralstonia spp. were identified using phenotypic and genotypic methods and were tested for the susceptibility towards antibiotics. The thesis also proposes a new method of genotypic indentification of R. respiraculi.
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Optimization of HPLC separation of penicillin derivatives
Šímová, Veronika ; Kalíková, Květa (advisor) ; Tesařová, Eva (referee)
Penicillins are part of β-lactam antibiotics, which are wide spread in human and veterinary medicine. The aim of this bachelor work was to develop and to optimize HPLC method for separation of four derivatives of penicillin - amoxicillin, 4-hydroxyphenoxymethylpenicillin, benzylpenicillin and phenoxymethylpenicillin. The reserved phase HPLC with octodecyl stationary phase was used in this work. The baseline separation of all analytes was not obtained in isocratic elution. For this reason, the gradient elution was examined on the baseline resolution of all analytes was achieved and the analysis time did not exceed 22 minutes. The stability of methanolic solutions of penicillins was tested during eight days. Key words: HPLC, antibiotics, derivatives of penicillins, reverse mod, stability
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Interaction of the pathogenic yeasts of the genus Candida with the bacterium Pseudomonas aeruginosa
Mynářová, Lenka ; Hodek, Petr (advisor) ; Šťovíček, Vratislav (referee)
Pathogenic yeasts of the genus Candida and the bacterium Pseudomonas aeruginosa are opportunistic pathogens and they often occur in the human micoflora as harmless commensals. They do not mean a threat for healthy humans but they can represent a true hazard for a person with supressed immunity. Cystic fibrosis (CF) is an incurable genetic disease and among others, it causes supression of immunity. Most of the patients with CF suffer from the chronical lung infections caused by the bacterium Pseudomonas aeruginosa. The pathogenic yeasts can coexist with the bacteria both in lungs of the CF patients, and on various surfaces, e.g. catheters used in hospitals. Therefore it is important to explore the mode of their interaction in the host body, as well as in vitro. Both Candida and Pseudomonas secrete a range of proteins that act as virulence factors, and also small molecules, which mediate the interaction and communication between microorganisms. We therefore tested cocultivation of two Candida species (C. albicans and C. parapsilosis) with P. aeruginosa in three different chemically defined liquid media. We monitored viability and survival of the individual species and analyzed the proteins secreted to the media. We found that P. aeruginosa secreted several hydrolytic enzymes, for example aminopeptidase,...
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Awareness of contagious disease in mind of pupils at basic and secondary schools.
Peštová, Ilona ; Rajsiglová, Ina (advisor) ; Fraiberk, Martin (referee)
Infectious diseases are a very hot topic nowadays in society. In recent years, there were several epidemics, infectious diseases (hepatitis A, Avian influenza, pandemic influenza A) and nobody knows when will the next "new" infection. At the outbreak of epidemics, there is great interest in the company to obtain information about the disease, but often also to unnecessary panic, because the media often publish incorrect information. It would be preferable, in order to improve public awareness and prevent the unnecessary spread of disease. Great emphasis should be given to prevent the disease - primarily on immunization, hygiene rules and principles of safe sex. Quality information should be mainly from teachers in teaching their pupils, as is clear from research books, infectious diseases are only mentioned in textbooks and the number of substantive information in them is missing. The fact that pupils of primary schools and grammar schools with basic information on infectious diseases do not meet in the classroom, evidenced by the results of a survey carried out in the 6th classes and first year at selected elementary schools and grammar schools in Prague. To raise awareness of the pupils in school was to create a methodical manual for teachers, which summarizes information about bacterial and...
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Characterization of N-demethyllincomycin-methyltransferase.
Poľan, Marek ; Najmanová, Lucie (advisor) ; Petříčková, Kateřina (referee)
Lincomycin is a naturally occurring member of a lincosamide group of antibiotics. The cluster of lincomycin biosynthetic gene was already decribed and the function of many of genes has been clarified. This work, "Characterization of N-demethyllincomycin-methyltransferase", is focused on the study of the final step of lincomycin biosynthetic pathway - the methylation of nitrogen atom from the pyrollo ring of the propylproline unit of the N-demethyllicomycin (NDL). The aim of this work was the characterization of the protein LmbJ, catalysing this final biosynthetic step. All the experiments were provided for the enzyme LmbJ with N-terminal histidine tag, which had been prepared by the heterologous expression in E.coli cells. The pH and temperature optimum was determined as well as the Michaelis constants for both substrates of the reaction - N-demethyllincomycin and S-adenosyl methionine (SAM - a methyl group donor). With the exception of the pH optimum, all specified parameters have markedly differed from the data published for the enzyme isolated from the natural source. Based on the comparison of electron microscopy, blue native gel electrophoresis and gel filtration results, the hypothetical model of the LmbJ quarternary structure was created. Majority of methyltranserases, so far described occure in...
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