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Mineral waters in the Bad Brambach - Skalná area, the current knowledges.
Turnová, Štěpánka ; Goliáš, Viktor (advisor) ; Hrkal, Zbyněk (referee)
This paper summarizes the findings of the mineral waters around Skalná - Bad Brambach. Bad Brambach are radon spa that work here since 1912. Source Wettinquelle is one of the strongest radon springs in the world. Around Skalná is anomalous quantities of uranium, however, that there occurs in such small particles that netěžitelné. Radon is a decay product of U238 series. Within the study area is also NPR Soos, who is known moffetes, but there also are so-called diatomaceous peak which is a dry salt lake containing diatoms. Area of the Nový Kostel is the area with the most frequent occurrence of earthquakes in the Czech Republic. Several earthquake swarms were recorded as well as the local populations. Recent earthquake activity has been recorded in 2011. There are also several other spa municipal Františkovy lázně, Mariánske lázně, Karlovy Vary and other radon baths, which already are but a little further, Jáchymov.
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Age estimation in two recent European population by assessment of permanent tooth mineralization
Černá, Magdaléna ; Velemínská, Jana (advisor) ; Stránská, Petra (referee)
Age estimation is a common requirement in forensic, bioarcheological and biomedical practice. This master thesis deals with age estimation based on permanent tooth mineralization according to Demirjian et al. (1973). The research material consisted of orthopantomograms of 716 Czech and 743 French children aged between 4 and 15 years. The purpose of this study was to analyse the suitability of the original French-Canadian standards for age estimation (Demirjian a Goldstein, 1976) and the recent Belgian standards (Willems et al., 2001) in Czech and French population. Another aim of the study was to evaluate the accuracy of the neural network method that represents a completely new approach in data prediction. In order to express the accuracy of estimate we used mean and median of difference between chronological and dental age, and RMS error. Using logistic regression, differences in tooth mineralization between Czech and French population and between girls and boys were also evaluated. Our results indicate that the French-Canadian standards gave a consistent overestimation of dental age compared with chronological age. Mean difference was 0,33 years for Czech children and 0,45 and 0,46 years for French girls and boys, respectively. We found that Willem's method and neural network method were more...
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Application of Atomic Absorption Spectrometry for Determination of Selected Elements from Potential Ecological Burden Area
Juránková, Lada ; Hraníček, Jakub (advisor) ; Šíma, Jan (referee)
This work is focused on the determination of selected (chemical) elements (Fe, Mn, Zn, Cr, Na, K, Mg a Ca) in a potential ecological burden area using flame atomic absorption spectrometry as a detection technique. A potential ecological burden is represented by tires which are used as a construction material or as an environment surrounding for growing decorative and consumable plants. Analytes were determined in plant origin samples. Firstly, the samples were dried in a laboratory electric dryer. The weighted amount of a dried sample (approximately 0.2 g) was digested inside a microwave decomposition device with an addition of nitric acid and hydrogen peroxide. An optimization of experimental parameters was done for each of the elements before sample measuring. A burner height was optimized and 8 mm height was chosen as a optimum for most elements. Under the optimum condition basic characteristics were measured for each element separately. These characteristics included limits of detection that were: 0.05; 0.01; 0.08; 0.21; 0.02; 0.01; 0.01; 0.01 mg L-1 for the following elements: Fe, Mn, Zn, Cr, K, Ca, Mg and Na, respectively. The limits of quantification, sensitivity and repeatability were determined for each element as well. A significant attention was paid to the content of zinc in the studied...
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Age estimation based on human dentice
Černá, Magdaléna ; Velemínská, Jana (advisor) ; Brůžek, Jaroslav (referee)
Age estimation plays a very important role not only in forensic and biomedicinal anthropology, but also in other disciplines, such as bioarcheologie or paleoanthropology. Teeth are considered the most reliable age indicators during childhood and adolescence. Teeth are resistant to the effects of external and internal factors, and because of that they decay a long time after a death of an individual. While age estimation in children and adolescents can be based on mineralization or eruption of the teeth, in adults it is focused on retrogressive changes in dentition. There have been established several methods and their modification for age assessment based on these criteria. This thesis aims to generally summarize tthe principles and methods of age estimation based on dentition both in immature and adult individuals.
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Utilization of forensic dentistry in indentification of individuals
Fialková, Martina ; Velemínská, Jana (advisor) ; Stránská, Petra (referee)
Forensic identification and age estimation has a significant role in cases when the unknown deceased body is found, after mass disasters when it is necessary to distinguish victims, but also in guestion of imigrants. And just these areas are very important part of forensic odontology, because dental development like a complex proces takes place from early foetal life to approximately 20 years of age is less affected by endocrine diseases or nutritional variations than other tissues. Dental age estimate is fundamental mainly in cases of children and young people, which teeth are still growing and they are in different developmental stages. On basis of these stages is possible to obtain very accurate results.
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Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
Doubková, Martina ; Bačáková, Lucie ; Pařízek, Martin ; Vandrovcová, Marta ; Musílková, Jana ; Lisá, Věra ; Gabor, R. ; Marvan, J.
Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants.
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Determination of toxic elements in wines
Hajdučková, Iva ; Vespalcová, Milena (referee) ; Diviš, Pavel (advisor)
The aim of this study was to determine the occurrence of selected toxic elements in wine. The theoretical part described technology of wine production and selected toxic elements (cadmium, lead, copper, zinc, nickel and chromium) in the terms of their impact on wine quality and their effects on the human organism. It provides an overview of instrumental analytical methods, which can be used for the analysis of toxic metals. In the experimental part inductively coupled mass spektrometry technique was used for the analysis of wine samples. The measured values are statistically processed and compared with current legislation.
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Selected inorganic pollutants in foodstuffs
Kroupová, Kateřina ; Vítová, Eva (referee) ; Vitoulová, Eva (advisor)
This bachelor thesis is focus on occurrence of inorganic pollutants in foodstuffs. The introduction is dedicated to apperance of individual selected elements in foodstuffs. Concentration of these elements is very important factor for examination of toxic effects for the human organism. If we want to avoid the harmful effects to the human organism, we have to define border limits. Work is based on the legislative of Czech republic. Second part of this study is focused on the biological monitoring. The biological monitoring is important for precautionary measures. In the third part of this study are described toxical effects of these elements to the human organism. Before qualitative and quantitative analysis by instrumental techniques is important to properly take the sample. In the fourth and fifth part of this work are mentioned methods used for determination of these elements and metods of taking samples. This bachelor thesis provide complex view on the problems of pollutants in foodstuffs and it should be used as a base for following diploma thesis.
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