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Study of the mammalian oncogenic transcription factors in the yeast model
Novák, Josef ; Zámostná, Blanka (advisor) ; Šťovíček, Vratislav (referee)
Yeast serves as a useful tool for studying cellular processes and therefore a large amount of techniques and protocols has been developed. There are special methods for studying the transcriptional factors in yeast, such as modified yeast two-hybrid screens, yeast one-hybrid screens and systems studying ability of transcription factors to transactivate a reporter gene. Oncogenes from AP-1 complex, Myc and Myb protein families are described in this work. Using a yeast model the structural-functional properties of proteins can be easily studied and in some cases even their ability of oncogenic transformation can be predicted (FASAY or ability of c-Myc to transactivate a reporter gene). However, results from yeast models must be confirmed in mammalian cells. 1
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The Influence of the Economic Crisis on Corporate Social Responsibility Activities
Potůčková, Petra ; Rynda, Ivan (advisor) ; Novák, Josef (referee)
The aim of this thesis is to introduce the concept of Corporate Social Responsibility (CSR) and describe the relevance of corporate social responsibility activities. Due to the fact that, one of the basic characteristics of Corporate Social Responsibility, is long-term in character, which requires a dedication to CSR activities, even in times of financial difficulties when company does not have the optimal results, this thesis shows how selected companies fulfill the concept of CSR. The theoretical part is divided into two parts - the first is the concept of Corporate Social Responsibility, which focuses on the development, pillars, benefits and concepts associated with this concept. I deal with crisis and describe financial crisis, economic crisis, its development, causes and consequences in the next part. The empirical part of this thesis is based on the methodological triangulation - expert interviews, documented examination and comparison. This thesis sampled five selected companies and shows how these companies perceive CSR, what activities they have implemented, how to cope with the crisis that affected them and how it affected the implementation of CSR activities. Another benefit of this thesis is to expand knowledge about values of companies around the city of Kolín. The results of this...
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Analysis of indicators of the social environment in the municipalities on the basis of various criteria for use at the local level
Galgonek, Dina ; Janoušková, Svatava (advisor) ; Novák, Josef (referee)
This diploma thesis deals with analysis of indicators of the social environment in small municipalities with up to 2 000 inhabitants based on various criteria for local use. The aim of the thesis is to propose an indicator set, which will best meet the requirements of such set in terms of applied criteria. The research question is: What indicators should be included in an indicator set for assessing the social environment of municipalities with up to 2 000 inhabitants to suit the differences and needs of these communities? The research problem addressed in the thesis concerns the possibility of measuring social sustainability at the local level, focusing on small municipalities with up to 2 000 inhabitants in the Czech Republic. The thesis also focuses on the possibility of projecting SDGs into this set. It is a qualitative research that involves the use of methods of document analysis, comparison, description, non-participant observation and semi-structured interviews. Based on the first four methods and theoretical knowledge, a preliminary design of the indicator set was created. Within the framework of semi-structured interviews, this design was discussed with representatives of selected small municipalities and other stakeholders. The edited set represents the final indicator set of the social...
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Generation of conditional animal mutants to study gene function in vivo
Herrmannová, Pavlína ; Sedláček, Radislav (advisor) ; Novák, Josef (referee)
Conditional gene targeting allows spatial and temporal control of genetic modifications and is used to study gene functions in specific tissues or cell types. Gene targeting may lead to inactivation of the gene by insertions or deletions. Conditional gene targeting uses various methods for generation of transgenic mutant animals, such as technology of targeted disruption of gene using embryonic stem cells, methodology based on bacterial artificial chromosomes, or a new revolutionary technology of targeted disruption of genes using programmable nucleases, which is rapidly evolving and seems to be more efficient and cheaper method for conditional gene targeting. The aim of this work is to overview methods and technologies for generation conditional animal models, and overview conditional recombination systems with emphasis on inducible systems, and also provides a summary of the main international resources for rodent mutagenesis. Key words: transgenic animal model, gene, targeting, conditional allele
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Development of agriculture in Czechia after 1990: on example of theTábor region
Novák, Josef ; Jančák, Vít (advisor) ; Zavadil, Tomáš (referee)
This bachelor thesis deals with the transformations of Czech agriculture during the last century and especially after 1990. The whole period is accompanied by major social changes that have affected the whole landscape and people. It is the end of the Great War, the communist putsch, the collectivization and the restitution processes that shaped our country and built agriculture here. Due to political and natural forces, Czech agriculture has changed the landscape, and this work aims to assess selected influences. As part of the analysis, the Tábor area, which represents a traditional agricultural region, will be evaluated. The model area will be introduced in more detail and placed in the context of these changes and agriculture in our country. Key words: agriculture, Táborsko, land use, transformation, agroturism, land
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Noncanonical functions of IL-1α
Novák, Josef
1α (IL 1α) is a multifunctional cytokine 1α is 1α independent on the receptor sig 1α is responsible for 1α to the plasma membrane. 1α activates express κB, binds to 1α 1α 1α to the plasma membrane 1α to signal 1α is required for membrane 1α exter 1α anchoring 1α 1α 1α with tumor suppressor p53 following genotoxic stress is further described in human cell 1α coloca
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Noncanonical functions of IL-1α
Novák, Josef ; Pospíšek, Martin (advisor) ; Černý, Jan (referee) ; Brdička, Tomáš (referee)
1α (IL 1α) is a multifunctional cytokine 1α is 1α independent on the receptor sig 1α is responsible for 1α to the plasma membrane. 1α activates express κB, binds to 1α 1α 1α to the plasma membrane 1α to signal 1α is required for membrane 1α exter 1α anchoring 1α 1α 1α with tumor suppressor p53 following genotoxic stress is further described in human cell 1α coloca
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Integration of Business Intelligence Tools into IS
Novák, Josef ; Bartík, Vladimír (referee) ; Stryka, Lukáš (advisor)
This Master's Thesis deals with the integration of Business Intelligence tools into an information system. There are concepts of BI, data warehouses, the OLAP analysis introduced as well as the knowledge discovery from databases, especially the association rule mining. In the chapters focused on practical part of the thesis, the design and implementation of resultant application are depicted. There are also the applied technologies like i.e. Microsoft SQL Server 2005 described.
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Measurement of Production Overall Equipment Effectiveness
Žanda, Miroslav ; Novák, Josef (referee) ; Píška, Miroslav (advisor)
The diploma work deals with measurement of Production Overall Equipment Effectiveness in manufacturing company Alfa. Firstly, is processed the theoretical part concerning general production, TPM, lean manufacturing and OEE. After than is described the company, monitored device and technology used by the device. In the final section is evaluated the OEE for January, February and March; analyze downtimes, mistakes and losses and their solutions are proposed. The last chapter is devoted to the economic evaluation of the proposal.
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Analysis of indicators of the social environment in the municipalities on the basis of various criteria for use at the local level
Galgonek, Dina ; Janoušková, Svatava (advisor) ; Novák, Josef (referee)
This diploma thesis deals with analysis of indicators of the social environment in small municipalities with up to 2 000 inhabitants based on various criteria for local use. The aim of the thesis is to propose an indicator set, which will best meet the requirements of such set in terms of applied criteria. The research question is: What indicators should be included in an indicator set for assessing the social environment of municipalities with up to 2 000 inhabitants to suit the differences and needs of these communities? The research problem addressed in the thesis concerns the possibility of measuring social sustainability at the local level, focusing on small municipalities with up to 2 000 inhabitants in the Czech Republic. The thesis also focuses on the possibility of projecting SDGs into this set. It is a qualitative research that involves the use of methods of document analysis, comparison, description, non-participant observation and semi-structured interviews. Based on the first four methods and theoretical knowledge, a preliminary design of the indicator set was created. Within the framework of semi-structured interviews, this design was discussed with representatives of selected small municipalities and other stakeholders. The edited set represents the final indicator set of the social...
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