Original title:
Analysis of plant membrane lipids by RP-HPLC/HR-ESI-MS
Authors:
Zábranská, Marie ; Vrkoslav, Vladimír ; Cvačka, Josef Document type: Papers Conference/Event: International Students Conference "Modern Analytical Chemistry" /7./, Prague (CZ), 2011-09-29 / 2011-09-30
Year:
2011
Language:
eng Abstract:
Monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) are glycolipids forming the major part of biological membranes in photosynthesizing organisms. The individual species differ from fatty acids linked to sn-1 and sn-2 possition on glycerol backbone. Complexity of their mixtures is increased by enzymatic or chemical oxidation of unsaturated species. The aim of this work was to develop and optimize RP-HPLC/HR-ESI-MS2 methods for analysis of oxidized and non-oxidized MGDGs and DGDGs in plant samples. MGDGs and DGDGs were isolated from Arabidopsis thaliana and Melissa officinalis.
Keywords:
digalactosylglycerols; high-resolution mass spectrometry; monogalactosyldiacylglycerols; RP-HPLC Project no.: CEZ:AV0Z40550506 (CEP), SVV261204, GA203/09/0139 (CEP) Funding provider: GA KU, GA ČR Host item entry: Proceedings of the 7th International Students Conference "Modern Analytical Chemistry", ISBN 978-80-7444-010-6
Institution: Institute of Organic Chemistry and Biochemistry AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0201265