Original title: Analysis of plant membrane lipids by RP-HPLC/HR-ESI-MS
Authors: Zábranská, Marie ; Vrkoslav, Vladimír ; Cvačka, Josef
Document type: Papers
Conference/Event: International Students Conference "Modern Analytical Chemistry" /7./, Prague (CZ), 2011-09-29 / 2011-09-30
Year: 2011
Language: eng
Abstract: Monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) are glycolipids forming the major part of biological membranes in photosynthesizing organisms. The individual species differ from fatty acids linked to sn-1 and sn-2 possition on glycerol backbone. Complexity of their mixtures is increased by enzymatic or chemical oxidation of unsaturated species. The aim of this work was to develop and optimize RP-HPLC/HR-ESI-MS2 methods for analysis of oxidized and non-oxidized MGDGs and DGDGs in plant samples. MGDGs and DGDGs were isolated from Arabidopsis thaliana and Melissa officinalis.
Keywords: digalactosylglycerols; high-resolution mass spectrometry; monogalactosyldiacylglycerols; RP-HPLC
Project no.: CEZ:AV0Z40550506 (CEP), SVV261204, GA203/09/0139 (CEP)
Funding provider: GA KU, GA ČR
Host item entry: Proceedings of the 7th International Students Conference "Modern Analytical Chemistry", ISBN 978-80-7444-010-6

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0201265

Permalink: http://www.nusl.cz/ntk/nusl-71493


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers