Original title: Epitachophoretic preconcentration of proteins
Authors: Hrušková, Helena ; Voráčová, Ivona ; Foret, František
Document type: Papers
Conference/Event: International Students Conference 'Modern Analytical Chemistry' /17./, Praha (CZ), 20210916
Year: 2021
Language: eng
Abstract: In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide low analysis time, narrow shape of the protein zone, and eliminate overheating at the center of the device. In the next step, the developed method was tested on the preconcentration of proteins: cytochrome c, myoglobin, and hemoglobin. After several adjustments myoglobin and hemoglobin were also uccessfully preconcentrated. After evaluation of pilot results, this technique provides up to fiftyfold preconcentration and recovery of over 70 % for each protein. The next plan is to preconcentrate proteins from complex biological matrices such as urine or blood plasma.
Keywords: epitachophoresis; isotachophoresis; preparative concentration
Host item entry: Proceedings of the 17th International Students Conference “Modern Analytical Chemistry”, ISBN 978-80-7444-089-2

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0322598

Permalink: http://www.nusl.cz/ntk/nusl-508167


The record appears in these collections:
Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers