Original title: Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis
Authors: Vaněčková, T. ; Zítka, J. ; Hlaváček, Antonín ; Adam, V. ; Vaculovičová, M.
Document type: Papers
Conference/Event: MendelNet 2017: International PhD Students Conference /24./, Brno (CZ), 20171108
Year: 2017
Language: eng
Abstract: Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.
Keywords: capillary electrophoresis; fluorescence spectrometry; upconversion nanoparticles
Project no.: GBP206/12/G014 (CEP)
Funding provider: GA ČR
Host item entry: MendelNet 2017: Proceedings of International PhD Students Conference, ISBN 978-80-7509-529-9

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf
Original record: http://hdl.handle.net/11104/0285736

Permalink: http://www.nusl.cz/ntk/nusl-385084


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers