Original title:
Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis
Authors:
Vaněčková, T. ; Zítka, J. ; Hlaváček, Antonín ; Adam, V. ; Vaculovičová, M. Document type: Papers Conference/Event: MendelNet 2017: International PhD Students Conference /24./, Brno (CZ), 20171108
Year:
2017
Language:
eng Abstract:
Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.
Keywords:
capillary electrophoresis; fluorescence spectrometry; upconversion nanoparticles Project no.: GBP206/12/G014 (CEP) Funding provider: GA ČR Host item entry: MendelNet 2017: Proceedings of International PhD Students Conference, ISBN 978-80-7509-529-9