National Repository of Grey Literature 85 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
The Pied Piper of Marina Tsvetaeva: Genesis, Reception and Translations of Poems with Commentary
Zakiyanov, Oskar ; Hlaváček, Antonín (advisor)
Russian poet Marina Ivanovna Tsvetaeva (1892-1941) is one of the world's most prominent authors of the early 20th century. The Pied Piper (Ratcatcher) is the magnum opus of her work. The aim of my thesis was to literally translate the poem and provide a detailed commentary on the poem. Translation is designed for the future Slavonic Studies, but also for the wider public. The translation is a kind of proposal and could be used as the default text for a possible poetic translation of the poem into Czech. The intention of the commentary is to point out the relationships of the Tsvetaeva's Pied Piper and possible sources of inspiration of the texts of other authors or her own original works. The commentary also explains the concepts specific to the creation of Tsvetaeva and her characteristic individual poetic methods. The commentary, however, provide, nearly no interpretation. It is used only as a supplementary comment on individual parts in order to enable better understanding of the original text and the translation. The first chapters deal with the history of the poem, its genesis, and reception by critics and writers. Followed by technical notes to the translated text, where a reader would find an explanation of the peculiarities of the original text, including syntactic difficulties, which are the clues...
The influence of Thomas Moore's work on Russian poetry of the 19th century
Ataniyazova, Diana ; Vasilyeva, Elena (advisor) ; Hlaváček, Antonín (referee)
This bachelor thesis studies the influence of the work of the Irish poet Thomas More on the development of Russian poetry in the 19th century. The aim of the thesis is to establish the prerequisites for influence, as well as the consequences of this influence in Russian literature. The work consists of three parts. In the first part, we got acquainted with the facts from the biography of Thomas More, which subsequently left a mark in the works of the author. In addition, we examined the poet's work from the point of view of Russian literary criticism. It is worth noting here that close attention to More's work flared up during the period of his greatest fame. The cycle of poems "Irish Melodies" served as inspiration not only for Russian society, but also interested many other consciousness in European countries. In the second part, we examined how the reforms of Peter I contributed to the emergence of Westernism and Slavophilism and how these tendencies subsequently influenced the perception of the Irish poet by the literary society in Russia, as well as how the traditions of Mohr's work are reflected in the poetry of M. Lermontov and A. Pushkin. The third part is devoted to the history of translations of Thomas Moore's works into Russian by such masters as V. Zhukovsky and I. Kozlov. The final...
Photon-upconversion scanner for multiplexed imaging
Hlaváček, Antonín ; Křivánková, Jana ; Foret, František
In analytical chemistry, multiplexed assays facilitate parallel detection of analytes. The multiplexing reduces the consumption of sample, reagents, and other resources and can be applied for medical diagnostics, the studies of biomolecule interactions, single-cell and singlemolecule assays, environmental monitoring, pathogen detection, multiparameter chemical and biological assays and screening of chemical libraries.
Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces
Hlaváček, Antonín ; Křivánková, Jana ; Foret, František
Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of UCNPs include near-infrared excitation, multiple and narrow emission bands, negligible autofluorescence and high stability, which make UCNPs ideal luminescence label for use in biological and chemical assays. These assays - e.g. upconversion-linked immunosorbent assay, western blot, lateral flow assay, gel electrophoresis, thin layer chromatography - commonly require the scanning of a planar surface with a high spatial resolution and an excellent sensitivity. The availability of commercial equipment is recently limited because of the novelty of the photon-upconversion phenomenon. Therefore, we report on the construction of photon-upconversion laser scanner. The scanner consists of a laser scanning head, which is attached to a xy-moving stage. The scanning head itself is constructed as an epiluminescence detector with excitation wavelength of 976 nm. A CCD array spectroscope is connected to the laser head and serves as a sensitive detector of photon-upconversion luminescence. The scanner possesses a spatial resolution of 200 μm, the scanning rate is up to 57 points per second and the sensitivity reaches down to single photon-upconversion nanoparticle.
Photon-upconversion nanoparticles for single-mocule immunosensing of cancer biomarkers and bacteria
Farka, Z. ; Mickert, M. J. ; Hlaváček, Antonín ; Poláchová, V. ; Kostiv, Uliana ; Pastucha, M. ; Horák, Daniel ; Gorris, H. H. ; Skládal, P.
The recent progress in the field of immunoassays has been driven by introduction of various kinds of nanomaterials. In particular, photon-upconversion nanoparticles (UCNPs) proved to be excellent immunoassay labels due to their ability to emit light of shorter wavelengths under near-infrared excitation (anti-Stokes emission), which prevents autofluorescence, minimizes light scattering, and thus reduces the optical background interference. These unique photoluminescent properties allow counting of individual biomolecules labeled with UCNPs by conventional wide-field epiluminescence microscopy and enable the development of single-molecule (digital) immunoassays. We have introduced a novel label based on UCNPs conjugated with streptavidin via poly(ethylene glycol) and applied it in a digital upconversion-linked immunosorbent assay (ULISA) for the detection of a cancer biomarker prostate specific antigen (PSA). The digital readout based on counting of individual immunocomplexes improved the sensitivity 16× compared to conventional analog readout and allowed to reach a limit of detection (LOD) of 23 fg·mL−1 (800 aM). Human serum samples were successfully analyzed achieving an excellent correlation with electrochemiluminescence reference method. The conjugates of UCNPs with streptavidin are also suitable for the detection of pathogenic bacterium Melissococcus plutonius, the causative agent of honeybee disease European foulbrood. The ULISA assay provided an LOD of 340 CFU·mL−1 and successfully analyzed real samples of bees, larvae and bottom hive debris. Due to the high reliability and relatively simple detection scheme, the digital ULISA can pave the way for a new generation of digital immunoassays with a strong potential for commercialization.
LIBS assessment of spatial photon-upconversion nanoparticle distribution in model plants (R. sativus and L. minor)
Modlitbová, P. ; Novotný, K. ; Hlaváček, Antonín ; Pořízka, P. ; Kaiser, J.
In the present study, radish (Raphanus sativus L.) and common duckweed (Lemna minor L.) were treated with an aqueous dispersion of carboxylated silica-coated photon-upconversion nanoparticles containing rare-earth elements (Y, Yb, and Er). The total content of rare earths and their bioaccumulation factors were determined in the root, hypocotyl, and leaves of R. sativus after 72 hours, and in L. minor fronds after 168 hours. In R. sativus, translocation factors were determined as the ratio of rare earths content in hypocotyl versus roots and in leaves versus hypocotyl. The lengths of the root and hypocotyl in R. sativus, as well as the frond area in L. minor were monitored as toxicity end points. To distinguish rare-earth bioaccumulation patterns, two-dimensional maps of elemental distribution in the whole R. sativus plant and in L. minor fronds were obtained by using laser-induced breakdown spectroscopy with a lateral resolution of 100 μm. Obtained results revealed that the tested nanoparticles became adsorbed on L. minor fronds and R. sativus roots, and got transferred from roots through the hypocotyl into leaves in R. sativus. Our results show that bioaccumulation patterns and spatial distribution of rare earths in nanoparticle-treated plants differ from those of positive control (the mixture of YCl3, YbCl3, and ErCl3).
Christian motives in poetics of a novel Resurrection from Leo Tolstoy
Bojková, Larysa ; Vasilyeva, Elena (advisor) ; Hlaváček, Antonín (referee)
Resumé Tématem bakalářské práce jsou křesťanské motivy v poetice románu Lva Nikolajeviče Tolstého Vzkříšení. Cílem práce bylo sledovat přístup autora ke křesťanskému učení v románu. První část práce se věnuje historii pravoslavné církve v Rusku, jejím dogmatům a tradicím. Dále se zabývá pojetím křesťanského učení Tolstého a z něj vyplývající idey "neprotivení se zlu násilím". Další část práce se věnuje románu Vzkříšení. Zkoumá, jak jsou hlavní ideová východiska v románu vyjádřena. Analýza textu odráží autorův negativní pohled na soudní systém. Lev Nikolajevič Tolstoj odsuzuje jeho nemorálnost a neefektivnost. Mluví o tom, že má soudní systém rozsáhlé negativní účinky na soudce, advokáty i všechny ostatní účastníky soudních řízení a osob součinných při výkonu trestů. Zákon není podle mínění Tolstého schopen spravedlivě potrestat zločince. Život odsouzených v nápravných zařízeních zcela znemožňuje nápravu. Rozřešení tohoto morálního a sociálního problému je spojeno s myšlenkami obsaženými v Matoušově evangeliu, což je také obsaženo v závěru románu. Dalším cílem práce byla analýza duchovního a morálního vývoje hlavních postav. U Maslovové i Něchljudova byl zjištěn dobrý duchovní stav do společně strávené noci. Poté lze u Maslovové pozorovat prudký obrat k nemorálnímu způsobu života. V Něchljudovově případě...
Typology of the Characters and Their Interpersonal Relationships in A. P. Chekhov's "Cherry Orchardˮ
Hovorková, Karolína ; Hlaváček, Antonín (advisor) ; Vasilyeva, Elena (referee)
The bachelor thesis is focused on the typology of characters and their interpersonal relationships in the last Anton Chekhov's drama "Cherry Orchard". The types of characters and their relationships are compared to characters and relationships in other Chekhov's dramas. Furthermore, the work takes into account Chekhov's contribution to the drama in general and it shows how the author perceived space and time which were also represented by the characters themselves. The analysis, compilation and comparison will be used in the processing, the knowledge will be demonstrated on practical examples, which become a part of the theoretical part of the thesis. The work will be based on foreign and Czech publications on typology of characters and their relationships in Chekhov's dramas. KEYWORDS: typology of the characters, interpersonal relationships, A. Chekhov, Cherry Orchard

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5 Hlaváček, Adam
3 Hlaváček, Aleš
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