Original title: Measurement of Intensity Profile in the Focal Region of Microscope Objective
Authors: Šerý, Mojmír ; Ježek, Jan ; Jákl, Petr ; Jonáš, Alexandr ; Zemánek, Pavel ; Liška, M.
Document type: Papers
Conference/Event: Nové trendy ve fyzice, Brno (CZ), 2001-11-15 / 2001-11-16
Year: 2001
Language: eng
Abstract: This work describes an experimental method for the measurement of the profile of the laser beam focused by an immersion microscope objective. The method makes use of the two-photon excited fluorescence emitted by a 200 nm dyed polystyrene bead fixed to the cover slip. A piezo driven stage is used for scanning of the bead through the focal volume of an infrared laser beam and the excited two-photon fluorescence is detected by a photomultiplier.
Keywords: focused beam; intensity measurement; two-photon fluorescence
Project no.: CEZ:AV0Z2065902 (CEP)
Host item entry: Sborník příspěvků konference Nové trendy ve fyzice, ISBN 80-214-1992-X
Note: Související webová stránka: mailto:sery@isibrno.cz

Institution: Institute of Scientific Instruments AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0101037

Permalink: http://www.nusl.cz/ntk/nusl-29517


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Research > Institutes ASCR > Institute of Scientific Instruments
Conference materials > Papers