Original title: Physical and biocatalytic properties of polyvinyl alcohol lens-shaped particles versus spherical polyelectrolyte complex microcapsules
Authors: Schenkmayerová, A. ; Bučko, M. ; Gemeiner, P. ; Treľová, D. ; Lacík, I. ; Chorvát Jr., D. ; Ačai, P. ; Polakovič, M. ; Lipták, L. ; Rebroš, M. ; Rosenberg, M. ; Štefuca, V. ; Neděla, Vilém ; Tihlaříková, Eva
Document type: Papers
Conference/Event: Workshop of Interesting Topics of SEM and ESEM, Mikulov (CZ), 2014-08-26 / 2014-08-31
Year: 2014
Language: eng
Abstract: Direct comparison of key physical and chemical-engineering properties of two representative matrices for multipurpose immobilisations was performed for the first time. Polyvinyl alcohol lens-shaped particles LentiKats and polyelectrolyte complex microcapsules were characterised by advanced techniques with respect to the size distribution of the particles, their inner morphology as revealed by fluorescent probe staining, mechanical resistance, size-exclusion properties, determination of effective diffusion coefficient and environmental scanning electron microscope imaging. Recombinant cells E. coli overexpressing enzyme cyclopentanone monooxygenase were immobilised in polyelectrolyte complex microcapsules and LentiKats for comparison of their operational stability using model Baeyer-Villiger oxidation of (±)-cis-bicyclo[3.2.0]hept-2-en-6-one to regioisomeric lactones as important chiral synthons for potential pharmaceuticals.
Keywords: ESEM; SEM
Project no.: EE.2.3.20.0103
Funding provider: GA MŠk
Host item entry: Workshop of Interesting Topics of SEM and ESEM, ISBN 978-80-87441-12-1

Institution: Institute of Scientific Instruments AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0238653

Permalink: http://www.nusl.cz/ntk/nusl-177566


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