National Repository of Grey Literature 63 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Application of Environmental Scanning Electron Microscopy (ESEM) in the field of miniatures´ analysis: methodology for specific use in miniature painting research
Hradil, David ; Hradilová, J. ; Neděla, Vilém ; Tihlaříková, Eva
The methodology deals with a completely new, unused procedure of non-invasive analysis of painted portrait miniatures, namely an environmental scanning electron microscope with energy-dispersive X-rays. spectrometer(EREM-EDS). EREM, unlike the conventional scanning electron microscope (REM), allows the analysis of ivory painting, which is not possible in a vacuum or low gas pressure environment, where there is a risk of deformation and damagedue to drying of this biological material. The methodology is divided into two parts - the first describes the study of morphological details at high magnification (eg. the possibility of distinguishing different types of biological substrates), the second is the implementation of elemental analysis in high spatial resolution.
SMV-2021-06: EDS analysis of industrial samples
Neděla, Vilém ; Tihlaříková, Eva
The user workshop aimed at handling of the software Esprit 2.2. functioning as a component part of X-Ray EDS analyzer (firm Bruker). The workshop includes the practical demonstrations of elements analyses of samples specially chosen by customer. Further, the workshop is aimed at the basic routines of the morphological analysis of these samples by means of scanning electron microscope.
SMV-2021-05: CLSM of miniature painting
Neděla, Vilém ; Olbert, Martin
The samples were analyzed in the confocal laser scanning microscope equipped with the special sample holder for gripping of miniatures compatible with electron microscope. The surface microstructure as well as the individual coloured zones of the samples were analyzed.
Aplikace environmentální rastrovací elektronové mikroskopie (EREM) v oboru analýzy miniatur: metodika pro specifické použití ve výzkumu miniaturního malířství
Hradil, David ; Hradilova, Janka ; Neděla, Vilém ; Tihlaříková, Eva
The methodology deals with a completely new, unused procedure of non-invasive analysis of painted portrait miniatures, using environmental scanning electron microscope with an energy-dispersive X-ray spectrometer (ESEM-EDS). ESEM, unlike the conventional scanning electron microscope (SEM), allows the analysis of the painting on the ivory support, which is not possible in the gas pressure approaching the vacuum, where there is a risk of deformation and damage due to the drying of this biological material. Instead of an inert gas (nitrogen, argon), the aim is to use a mixture of nitrogen and water vapor at a defined pressure corresponding to the required relative humidity in the measuring chamber. This enables a completely non-invasive analysis of painted miniature objects on organic types of supports. This methodology is divided into two parts: morphological analysis and elemental composition analysis. The first case involves the study of morphological details at high magnification (e. g. the possibility of distinguishing different types of supports or characterizing their microstructure). The second part describes the implementation of elemental analysis in high spatial resolution (distinction of individual pigment grains).
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SMV-2021-04: Advanced EDX analysis of miniature painting in ESEM
Neděla, Vilém ; Tihlaříková, Eva
Samples were analysed in experimentally reached and optimised high pressure conditions of environmental scanning electron microscope and using EDS X-Ray micro-analyser.
SMV-2020-22: Morphological analysis of miniature painting using light microscopy and ESEM
Neděla, Vilém
Miniature painting were analysed in experimentally reached and optimised high pressure conditions of environmental scanning electron microscope and using light microscopy. Optimal gas pressure and humidity were set to minimise sample damage. Special detectors were used for low dose imaging of surface morphology.
SMV-2020-21: Elemental X-Ray analysis of miniature painting using ESEM
Neděla, Vilém
Miniature painting were analysed in experimentally reached and optimised high\npressure conditions of environmental scanning electron microscope and using EDS X-Ray micro-analyser.
SMV-2019-12: Morphological analysis of miniature painting
Neděla, Vilém
Miniature painting were analysed in experimentally reached and optimised high pressure conditions of environmental scanning electron microscope and using light microscopy. Optimal gas pressure and humidity were set to minimise sample damage. Special detectors were used for low dose imaging of surface morphology.
SMV-2019-11: Elemental X-Ray analysis of miniature painting
Neděla, Vilém
Miniature painting were analysed in experimentally reached and optimised high pressure conditions of environmental scanning electron microscope and using EDS X-Ray micro-analyser.
In vivo study of diatom assemblages using low temperature method for ESEM
Tihlaříková, Eva ; Neděla, Vilém ; Fránková, Markéta
Diatoms are the most species-rich algal group represented by 12 000 described species and are recognized as powerful bio indicators and used for water quality monitoring. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating.This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of whole intact diatom cells, not only empty diatom frustules, extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes) and whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating and observation of these organisms in their native wet state adhered directly on the host plant is not yet common. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method (LTM) for the ESEM.

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