Název:
Iron oxide nanoparticle modified monolithic pipette tips for selective enrichment of phosphopeptides
Autoři:
Křenková, Jana ; Foret, František Typ dokumentu: Příspěvky z konference Konference/Akce: CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./, Brno (CZ), 2012-11-01 / 2012-11-02
Rok:
2012
Jazyk:
eng
Abstrakt: We have developed iron oxide nanoparticle modified monolithic pipette tips for selective and efficient enrichment of phosphopeptides. Iron oxide nanoparticles were synthesized using a co-precipitation method and stabilized by citrate ions. A stable coating of nanoparticles was obtained via multivalent interactions of citrate ions on the nanoparticle surface with a quaternary amine functionalized surface of the methacrylate based monolithic tips. The performance of the developed and commercially available tips was demonstrated with the enrichment of phosphorylated peptides from tryptic digests of caseins followed by MALDI/MS detection.
Klíčová slova:
iron oxide nanoparticles; monolith; phosphopeptide enrichment Číslo projektu: GAP301/11/2055 (CEP), GBP206/12/G014 (CEP), 2SGA2721 Poskytovatel projektu: GA ČR, GA ČR, Jihomoravský kraj Zdrojový dokument: CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-1-3 Poznámka: Související webová stránka: http://hdl.handle.net/11104/0215394
Instituce: Ústav analytické chemie AV ČR
(web)
Informace o dostupnosti dokumentu:
Dokument je dostupný v repozitáři Akademie věd. Původní záznam: http://hdl.handle.net/11104/0215394