Original title: Iron oxide nanoparticle modified monolithic pipette tips for selective enrichment of phosphopeptides
Authors: Křenková, Jana ; Foret, František
Document type: Papers
Conference/Event: CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./, Brno (CZ), 2012-11-01 / 2012-11-02
Year: 2012
Language: eng
Abstract: We have developed iron oxide nanoparticle modified monolithic pipette tips for selective and efficient enrichment of phosphopeptides. Iron oxide nanoparticles were synthesized using a co-precipitation method and stabilized by citrate ions. A stable coating of nanoparticles was obtained via multivalent interactions of citrate ions on the nanoparticle surface with a quaternary amine functionalized surface of the methacrylate based monolithic tips. The performance of the developed and commercially available tips was demonstrated with the enrichment of phosphorylated peptides from tryptic digests of caseins followed by MALDI/MS detection.
Keywords: iron oxide nanoparticles; monolith; phosphopeptide enrichment
Project no.: GAP301/11/2055 (CEP), GBP206/12/G014 (CEP), 2SGA2721
Funding provider: GA ČR, GA ČR, Jihomoravský kraj
Host item entry: CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-1-3
Note: Související webová stránka: http://hdl.handle.net/11104/0215394

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0215394

Permalink: http://www.nusl.cz/ntk/nusl-135549


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers