National Repository of Grey Literature 69 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Determination of manganese in food supplements by atomic absorption spectrometry
Melicharová, Zuzana ; Hraníček, Jakub (advisor) ; Šíma, Jan (referee)
EN The main content of this thesis was the determination of manganese in the form of manganese ions in food supplements by the atomic absorption spectrometry. It was necessary to find the optimum conditions for this method, to conduct interference studies, to find a suitable way of transferring samples into solution and to determin manganese to achieve this goal. Following parameters were examined in optimalization study: beam height above burner edge, burner horizontal position, acetylene flow rate, torch angle, and spectral interval width. Copper, iron, sodium and calcium ions were investigated as interferents as they were present at the highest concentration in selected food supplements. The conversion of solid samples into solution was carried out in water and nitric acid at a room temperature as well as elevated temperature. These food supplements were selected for analysis: Calibrum 50 Plus, Caltrate Plus, Center A to Iron, DAS Gesunde Plus A-Z Multispectrum, GS Extra Strong Multivitamin, Spectrum 50+ and Supradyn Energy Complex. The manganese assay was performed on a GBC 933 AA spectrophotometer. Standard solutions were measured to determine the detection limit and the limit of determination of the instrument. The detection limit was 0.001 mg/l and the limit of determination was 0.004 mg/l...
Development of electrophoretic method for determination of sulphate in blood plasma
Hnízdilová, Lucie ; Křížek, Tomáš (advisor) ; Hraníček, Jakub (referee)
Cílem této bakalářské práce bylo vyvinout metodu pro stanovení síranů v krevní plazmě pomocí kapilární zónové elektroforézy. Byla použita křemenná kapilára o vnitřním průměru 50 μm a efektivní délce 41,5 cm. Plazmatické proteiny byly sráženy acetonitrilem. Jako optimální základní elektrolyt byla určena 0,4M kyselina mravenčí s 15mM chromanem draselným. Separační napětí bylo 25 kV. Vzorek byl dávkován napětím -5 kV po dobu 40 s. K vyhodnocení byla využita nepřímá UV detekce při 260 nm. Vzhledem ke složité matrici je vhodné provést kvantifikaci metodou standardního přídavku. Po vztažení ploch píků na vnitřní standard (500μM chlorečnany) byla relativní směrodatná odchylka 5,97 %. Výhodou této metody oproti dříve používaným srážecím metodám je krátká doba analýzy a jednodušší úprava vzorku. Klíčová slova kapilární elektroforéza, anorganické ionty, sírany, krevní plazma
Sequential injection analysis capability in automation of analytical processes
Novosvětská, Lucie ; Sklenářová, Hana (advisor) ; Polášek, Miroslav (referee) ; Hraníček, Jakub (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate (married name/maiden name): Mgr. Lucie Novosvětská / Mgr. Lucie Zelená Supervisor: assoc. prof. PharmDr. Hana Sklenářová, Ph.D. Consultant: PharmDr. Petr Chocholouš, Ph.D. Title of dissertation thesis: Sequential injection analysis capability in automation of analytical processes This dissertation, composed as a commented collection of four scientific articles and one undergoing project, introduces four novel applications in the field of automated flow methods and one application in the field of chromatographic methods used in analytical part of a complex pharmacological study. It the field of pharmaceutical analysis, three works were successfully optimized, validated, and published in scientific journals. Those works include: a) Automation of permeation studies within a sequential injection analysis system connected to a liberation unit and carrying out experiments with living cells. b) Pharmacological study of an antiretroviral drug efavirenz including its determination by fast chromatographic method in three types of sample matrices - in a medium from permeation studies with cellular models, in a Krebs solution from rat placenta perfusions, and in placenta tissue lysates. c) Determination of...
Determination of rubidium in selected plant extracts by atomic absorption spectrometry
Šatrová, Lucie ; Hraníček, Jakub (advisor) ; Jelínek, Ivan (referee)
In this diploma thesis, rubidium in plant material samples was determined by atomic absoption spectrometry. Determination of plant material rubidium was performed on two different atomic absorption spectrometers (GBC 933 AA and ContrAA 700) for comparison. The selection of a proper method of atomization was essential, therefore optimizations for the flame atomizer and electrothermal atomizer were performed. On the GBC 933 AA, flame atomization was tested. The flow rate of the acetylene-air, vertical and horizontal flame profile, spectral interval width was optimized for the instrument. On the ContrAA 700, the conditions for flame atomization were optimized as well as for electrothermal atomization. The optimized parameters included the acetylene-air flow rate and the vertical flame profile again. For the electrothermal atomization, the temperature dependence of pyrolysis and the temperature dependence of atomization were optimized. Under experimentally determined optimal conditions, the determination of rubidium in fruit and vegetable juice samples was performed by the method of calibration curve. Rubidium usually accompanies toher alkali metals. In the absence of essential biogenic elements iportant for plant growth, rubidium is able to help out and take on the role of potassium.
Development of an ultra-high performance liquid chromatography for rifampicin determination
Alušicová, Sára ; Kozlík, Petr (advisor) ; Hraníček, Jakub (referee)
In this bachelor thesis, an ultra-high-performance liquid chromatography method (UHPLC) was developed to determine rifampicin in phosphate buffer (also called PBS). Rifampicin is a bactericidal antibiotic from the rifamycin family, which plays an important role in the treatment of tuberculosis. Optimized conditions for rifampicin determination in PBS were as follows: column kinetex UHPLC C18 100 (50x2.1 mm; 1.3 µm), mobile phase consisted of 10 mM aqueous ammonium formate buffer (pH 3.51) to which trifluoroacetic acid was added as an ion pairing agent (to a final concentration of 0.1%) (component A) and of ACN (component B). The measurement was carried out in the gradient elution mode of the mobile phase (time: 0-1-6-7-8-11, component B: 30,30,80,80,30,30). The flow rate was 0.300 ml / min at a column temperature of 30 ř C. The injection volume was 5µl. Under these optimized UHPLC conditions, a calibration dependence was measured in concenatraction range from 28,310-3 mg ml-1 to 4,4210-4 mg ml-1 . The limit of detection was 1,05∗10-4 mg ml -1 and the limit of quantification was 3,5∗10-4 mg ml -1 . Keywords: antibiotic, rifampicin, UHPLC
Novel fluorosensors based on naphthalimide derivatives
Garbárová, Veronika ; Dian, Juraj (advisor) ; Hraníček, Jakub (referee)
The aim of the diploma thesis was to prepare and characterize fluorescence sensor systems based on fluorophores and supramolecular components - cyclodextrins - attached to solid supports. In this work, functionalized 75 µm glass beads and two types of Nafion membranes were used as negatively charged solid surfaces. Derivatives of naphthalimide with a positively charged part allowing the attachment to surfaces were synthesized. The positively charged naphthalimide derivative was then attached on negatively charged surfaces via electrostatic interactions and the binding to the solid supports was examined using UV-VIS spectroscopy. Alternatively, the positively charged β-cyclodextrin derivative was also attached to the surface in an equimolar ratio with PNI-HEMPDA. The focus of the work were measurements of fluorescence sensor responses in the gas and liquid phase to linear alcohols - methanol to n-hexanol - for all sensor systems. For the studied sensor systems, the selected sensor parameters were determined - sensitivity, the limit of detection, linear dynamic range and a sensor response time constant. A practical application of the novel sensor system for the detection of ethanol in gasoline was examined. Fluorescence sensor response measurements of the prepared systems based on naphthalimide and...
Applied experiments with the use of measuring instruments for the analytical chemistry teaching at high schools
Břížďala, Jan ; Hraníček, Jakub (referee)
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Analysis of selected elements in dendrological sample of spruce trunk
Pastrňáková, Aneta ; Hraníček, Jakub (advisor) ; Šíma, Jan (referee)
The aim of this bachelor thesis was to determine selected elements, specifically iron, manganese, sodium, potassium, calcium, magnesium, barium, copper, zinc, strontium and aluminium in the sample of a spruce wood, taken from a dry tree from an area of South Bohemia. As a suitable and sufficiently sensitive method an atomic absorption spectrometry with flame atomization was used in this bachelor thesis. Before the analysis optimal conditions for the determination of these elements were experimentally found. The beam height above the burner edge and the acetylene flow rate were optimized depending on the type of oxidant used. Under the optimal conditions, the basic characteristics of the determination of individual elements were determined. These characteristics include mainly the limit of detection and quantification, sensitivity and repeatability. Optimized methods for determination of the selected elements were further applied for the analysis of real spruce wood samples. These samples were prepared by decomposition of the wood in a microwave device in the presence of nitric acid. In order to suppress interference, the suitable reagents were added to the analyzed samples to determine the selected elements (sodium, potassium, magnesium, calcium, barium). In total, 22 samples of wood of different...

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