Název: Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces
Autoři: Hlaváček, Antonín ; Křivánková, Jana ; Foret, František
Typ dokumentu: Příspěvky z konference
Konference/Akce: NANOCON 2019. International Conference on Nanomaterials - Research & Application /11./, Brno (CZ), 20191016
Rok: 2020
Jazyk: eng
Abstrakt: Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of UCNPs include near-infrared excitation, multiple and narrow emission bands, negligible autofluorescence and high stability, which make UCNPs ideal luminescence label for use in biological and chemical assays. These assays - e.g. upconversion-linked immunosorbent assay, western blot, lateral flow assay, gel electrophoresis, thin layer chromatography - commonly require the scanning of a planar surface with a high spatial resolution and an excellent sensitivity. The availability of commercial equipment is recently limited because of the novelty of the photon-upconversion phenomenon. Therefore, we report on the construction of photon-upconversion laser scanner. The scanner consists of a laser scanning head, which is attached to a xy-moving stage. The scanning head itself is constructed as an epiluminescence detector with excitation wavelength of 976 nm. A CCD array spectroscope is connected to the laser head and serves as a sensitive detector of photon-upconversion luminescence. The scanner possesses a spatial resolution of 200 μm, the scanning rate is up to 57 points per second and the sensitivity reaches down to single photon-upconversion nanoparticle.
Klíčová slova: hyperspectral imaging; laser scanner; nanoparticle; photon-upconversion
Číslo projektu: GJ18-03367Y (CEP)
Poskytovatel projektu: GA ČR
Zdrojový dokument: NANOCON Conference Proceedings - International Conference on Nanomaterials, ISBN 978-808729495-6, ISSN 2694-930X
Poznámka: Související webová stránka: https://www.confer.cz/nanocon/2019

Instituce: Ústav analytické chemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v repozitáři Akademie věd.
Původní záznam: http://hdl.handle.net/11104/0311152

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-432156


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav analytické chemie
Konferenční materiály > Příspěvky z konference