Název: Nanofiber scaffolds for local delivery of stem cells and immunosuppressive drugs for therapeutic purposes
Autoři: Zajícová, Alena ; Kössl, Jan ; Heřmánková, Barbora ; Boháčová, Pavla ; Holáň, Vladimír
Typ dokumentu: Příspěvky z konference
Konference/Akce: International Conference on Nanomaterials - Research and Application (NANOCON) /9./, Brno (CZ), 20171018
Rok: 2018
Jazyk: eng
Abstrakt: Cell-based therapy of local tissue injuries or damages requires application of stem cells and inhibition of harmful inflammatory reaction which could impede the healing process. To increase the effectiveness of this therapy, a local administration of drugs can avoid their side effects associated with a systemic treatment. A local therapy requires suitable carriers, which can transfer the cells and drugs to the site of injury. As a promising carriers turned out nanofiber scaffolds prepared by electrospinning technology from various types of polymers. The main advantage of this technology is a possibility to define properties of nanofiber scaffolds, optimal for the growth and transfer of stem cells, and which could incorporate various types of immunosuppressive drugs. Here we describe the formation and use of nanofiber scaffolds prepared by needleless electrospinning technology from poly (L-lactic acid) (PLA) which are loaded with immunosuppressive drug Cyclosporine A (CsA). We show that CsA-loaded nanofibers effectively and selectively inhibit proliferation of activated T cells and suppress the production of T cell cytokines in vitro. Simultaneously, these nanofiber scaffolds enable growth of mesenchymal stem cells (MSCs) and thus can serve as stem cell carriers. Moreover, using an experimental mouse model of skin transplantation, we showed that covering skin allografts with MSC-seeded and CsA-loaded nanofibers significantly inhibited the local production of pro-inflammatory cytokines IL-2, IL-17 and IFN-gamma, and supported healing. Thus, nanofiber scaffolds seeded with stem cells and loaded with CsA can serve as carriers of cells and drugs for a local cell therapy and for simultaneous effective immunosuppression.
Klíčová slova: cytokines; immunosuppressive drug CsA; nanofiber scaffold; stem cells
Číslo projektu: LO1508 (CEP)
Poskytovatel projektu: GA MŠk
Zdrojový dokument: Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application, ISBN 9788087294819

Instituce: Ústav experimentální medicíny AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0302803

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-408928


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav experimentální medicíny
Konferenční materiály > Příspěvky z konference