Název:
Epitachophoretic separation and concentration of large volume samples
Autoři:
Voráčová, Ivona ; Foret, František ; Datinská, Vladimíra ; Novotný, Jakub ; Gheibi, P. ; Berka, J. ; Astier, Y. Typ dokumentu: Příspěvky z konference Konference/Akce: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Rok:
2018
Jazyk:
eng
Abstrakt: There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also concentration of DNA from large volume samples is required. We have developed a new device suitable for separation, concentration and collection of ions from several milliliter sample volumes into microliter fractions. We have used a discontinuous electrolyte system comprising of the leading and terminating electrolytes utilized for concentration of samples containing DNA fragments. The method is simple, fast with high concentration factor and without extensive surface interactions.
Klíčová slova:
DNA; epitachophoretic separation; large sample volumes Číslo projektu: GBP206/12/G014 (CEP) Poskytovatel projektu: GA ČR Zdrojový dokument: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1