Název: Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Autoři: Farka, Z. ; Hlaváček, Antonín ; Mickert, M. J. ; Skládal, P. ; Gorris, H. H.
Typ dokumentu: Příspěvky z konference
Konference/Akce: NANOCON 2017. International Conference on Nanomaterials - Research and Application /9./, Brno (CZ), 20171018
Rok: 2018
Jazyk: eng
Abstrakt: Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg·mL−1.
Klíčová slova: antibody; bioconjugation; diclofenac; immunoassay; photon-upconversion nanoparticle
Číslo projektu: GBP206/12/G014 (CEP)
Poskytovatel projektu: GA ČR
Zdrojový dokument: NANOCON 2017: Conference Proceedings, ISBN 978-80-87294-81-9

Instituce: Ústav analytické chemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v repozitáři Akademie věd.
Původní záznam: http://hdl.handle.net/11104/0287392

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-387484


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav analytické chemie
Konferenční materiály > Příspěvky z konference